LOCUS       sequence-224116-       16816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal BeYDV-derived vector for cloning of up to four gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16816)
  AUTHORS   Prieto H, Miccono M, Aguirre C, Sanchez E, Castro A
  TITLE     Grape Biotechnology: Past, Present, and Future
  JOURNAL   In: Cantu D., Walker M. (eds) The Grape Genome. Compendium of Plant 
            Genomes. Springer, Cham.
REFERENCE   2  (bases 1 to 16816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "In: Cantu 
            D., Walker M. (eds) The Grape Genome. Compendium of Plant Genomes. 
            Springer, Cham."
COMMENT     Sequence Label: pGMV-U
FEATURES             Location/Qualifiers
     source          1..16816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    107..131
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1072..1417
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1360..1380
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1451..5554
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1451..5554
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5567..5587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5598..5846
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             6577..7368
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        7520..7595
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9445..9469
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10770..11399
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11828..12901
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12967..13161
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13397..13419)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13505..13645
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13560..13579
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13660..13677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13831..14419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13911..13930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15705..16499)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 cgccttacaa cggctctccc ctgacgccgt cccggactga tgggctgcct gtatcgagtg
       61 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
      121 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
      181 agagctcaaa gtttaacgcg ttagcagaag gcatgttgtt gtgactccga ggggttgcct
      241 caaactctat cttataaccg gcgtggaggc atggaggcag gggtattttg gtcattttaa
      301 tagatagtgg aaaatgacgt ggaatttact taaagacgaa gtctttgcga caaggggggg
      361 cccacgccga atttaatatt accggcgtgg ccccccctta tcgcgagtgc tttagcacga
      421 gcggtccaga tttaaagtag aaaatttccc gcccactagg gttaaaggtg ttcacactat
      481 aaaagcatat acgatgtgat ggtatttgat ggagcgtata ttgtatcagg tatttccgtt
      541 ggatacgaat tattcgtacg accctcggta ccgatcggcg cgccagattt gccttttcaa
      601 tttcagaaag aatgctaacc cacagatggt tagagaggct tacgcagcag gtatcatcaa
      661 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga
      721 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
      781 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt
      841 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa
      901 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
      961 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     1021 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     1081 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     1141 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     1201 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     1261 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     1321 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     1381 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcctg caggtagatc
     1441 gctcgtcgac atggataaga agtactctat cggactcgat atcggaacta actctgtggg
     1501 atgggctgtg atcaccgatg agtacaaggt gccatctaag aagttcaagg ttctcggaaa
     1561 caccgatagg cactctatca agaaaaacct tatcggtgct ctcctcttcg attctggtga
     1621 aactgctgag gctaccagac tcaagagaac cgctagaaga aggtacacca gaagaaagaa
     1681 caggatctgc tacctccaag agatcttctc taacgagatg gctaaagtgg atgattcatt
     1741 cttccacagg ctcgaagagt cattcctcgt ggaagaagat aagaagcacg agaggcaccc
     1801 tatcttcgga aacatcgttg atgaggtggc ataccacgag aagtacccta ctatctacca
     1861 cctcagaaag aagctcgttg attctactga taaggctgat ctcaggctca tctacctcgc
     1921 tctcgctcac atgatcaagt tcagaggaca cttcctcatc gagggtgatc tcaaccctga
     1981 taactctgat gtggataagt tgttcatcca gctcgtgcag acctacaacc agcttttcga
     2041 agagaaccct atcaacgctt caggtgtgga tgctaaggct atcctctctg ctaggctctc
     2101 taagtcaaga aggcttgaga acctcattgc tcagctccct ggtgagaaga agaacggact
     2161 tttcggaaac ttgatcgctc tctctctcgg actcacccct aacttcaagt ctaacttcga
     2221 tctcgctgag gatgcaaagc tccagctctc aaaggatacc tacgatgatg atctcgataa
     2281 cctcctcgct cagatcggag atcagtacgc tgatttgttc ctcgctgcta agaacctctc
     2341 tgatgctatc ctcctcagtg atatcctcag agtgaacacc gagatcacca aggctccact
     2401 ctcagcttct atgatcaaga gatacgatga gcaccaccag gatctcacac ttctcaaggc
     2461 tcttgttaga cagcagctcc cagagaagta caaagagatt ttcttcgatc agtctaagaa
     2521 cggatacgct ggttacatcg atggtggtgc atctcaagaa gagttctaca agttcatcaa
     2581 gcctatcctc gagaagatgg atggaaccga ggaactcctc gtgaagctca atagagagga
     2641 tcttctcaga aagcagagga ccttcgataa cggatctatc cctcatcaga tccacctcgg
     2701 agagttgcac gctatcctta gaaggcaaga ggatttctac ccattcctca aggataacag
     2761 ggaaaagatt gagaagattc tcaccttcag aatcccttac tacgtgggac ctctcgctag
     2821 aggaaactca agattcgctt ggatgaccag aaagtctgag gaaaccatca ccccttggaa
     2881 cttcgaagag gtggtggata agggtgctag tgctcagtct ttcatcgaga ggatgaccaa
     2941 cttcgataag aaccttccaa acgagaaggt gctccctaag cactctttgc tctacgagta
     3001 cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg accgagggaa tgaggaagcc
     3061 tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat ctcttgttca agaccaacag
     3121 aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag aaaatcgagt gcttcgattc
     3181 agttgagatt tctggtgttg aggataggtt caacgcatct ctcggaacct accacgatct
     3241 cctcaagatc attaaggata aggatttctt ggataacgag gaaaacgagg atatcttgga
     3301 ggatatcgtt cttaccctca ccctctttga agatagagag atgattgaag aaaggctcaa
     3361 gacctacgct catctcttcg atgataaggt gatgaagcag ttgaagagaa gaagatacac
     3421 tggttgggga aggctctcaa gaaagctcat taacggaatc agggataagc agtctggaaa
     3481 gacaatcctt gatttcctca agtctgatgg attcgctaac agaaacttca tgcagctcat
     3541 ccacgatgat tctctcacct ttaaagagga tatccagaag gctcaggttt caggacaggg
     3601 tgatagtctc catgagcata tcgctaacct cgctggatct cctgcaatca agaagggaat
     3661 cctccagact gtgaaggttg tggatgagtt ggtgaaggtg atgggaaggc ataagcctga
     3721 gaacatcgtg atcgaaatgg ctagagagaa ccagaccact cagaagggac agaagaactc
     3781 tagggaaagg atgaagagga tcgaggaagg tatcaaagag cttggatctc agatcctcaa
     3841 agagcaccct gttgagaaca ctcagctcca gaatgagaag ctctacctct actacctcca
     3901 gaacggaagg gatatgtatg tggatcaaga gttggatatc aacaggctct ctgattacga
     3961 tgttgatcat atcgtgccac agtcattctt gaaggatgat tctatcgata acaaggtgct
     4021 caccaggtct gataagaaca ggggtaagag tgataacgtg ccaagtgaag aggttgtgaa
     4081 gaaaatgaag aactattgga ggcagctcct caacgctaag ctcatcactc agagaaagtt
     4141 cgataacttg actaaggctg agaggggagg actctctgaa ttggataagg caggattcat
     4201 caagaggcag cttgtggaaa ccaggcagat cactaagcac gttgcacaga tcctcgattc
     4261 taggatgaac accaagtacg atgagaacga taagttgatc agggaagtga aggttatcac
     4321 cctcaagtca aagctcgtgt ctgatttcag aaaggatttc caattctaca aggtgaggga
     4381 aatcaacaac taccaccacg ctcacgatgc ttaccttaac gctgttgttg gaaccgctct
     4441 catcaagaag tatcctaagc tcgagtcaga gttcgtgtac ggtgattaca aggtgtacga
     4501 tgtgaggaag atgatcgcta agtctgagca agagatcgga aaggctaccg ctaagtattt
     4561 cttctactct aacatcatga atttcttcaa gaccgagatt accctcgcta acggtgagat
     4621 cagaaagagg ccactcatcg agacaaacgg tgaaacaggt gagatcgtgt gggataaggg
     4681 aagggatttc gctaccgtta gaaaggtgct ctctatgcca caggtgaaca tcgttaagaa
     4741 aaccgaggtg cagaccggtg gattctctaa agagtctatc ctccctaaga ggaactctga
     4801 taagctcatt gctaggaaga aggattggga ccctaagaaa tacggtggtt tcgattctcc
     4861 taccgtggct tactctgttc tcgttgtggc taaggttgag aagggaaaga gtaagaagct
     4921 caagtctgtt aaggaacttc tcggaatcac tatcatggaa aggtcatctt tcgagaagaa
     4981 cccaatcgat ttcctcgagg ctaagggata caaagaggtt aagaaggatc tcatcatcaa
     5041 gctcccaaag tactcactct tcgaactcga gaacggtaga aagaggatgc tcgcttctgc
     5101 tggtgagctt caaaagggaa acgagcttgc tctcccatct aagtacgtta actttcttta
     5161 cctcgcttct cactacgaga agttgaaggg atctccagaa gataacgagc agaagcaact
     5221 tttcgttgag cagcacaagc actacttgga tgagatcatc gagcagatct ctgagttctc
     5281 taaaagggtg atcctcgctg atgcaaacct cgataaggtg ttgtctgctt acaacaagca
     5341 cagagataag cctatcaggg aacaggcaga gaacatcatc catctcttca cccttaccaa
     5401 cctcggtgct cctgctgctt tcaagtactt cgatacaacc atcgatagga agagatacac
     5461 ctctaccaaa gaagtgctcg atgctaccct catccatcag tctatcactg gactctacga
     5521 gactaggatc gatctctcac agctcggtgg tgattcaagg gctgatccta agaagaagag
     5581 gaaggtttga ctcgagatat gaagatgaag atgaaatatt tggtgtgtca aataaaaagc
     5641 ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt
     5701 ctaatattaa atgaatgtaa gatcacatta taatgaataa acaaatgttt ctataatcca
     5761 ttgtgaatgt tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg
     5821 gactatggaa tatgattaaa gataaggagc tccggtgacg gacccatggg cttcgacttg
     5881 ccttccgcac aatacatcat ttcttcttag ctttttttct tcttcttcgt tcatacagtt
     5941 tttttttgtt tatcagctta cattttcttg aaccgtagct ttcgttttct tctttttaac
     6001 tttccattcg gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg
     6061 catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa
     6121 tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga
     6181 acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat
     6241 cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattggg
     6301 agaccaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6361 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6421 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6481 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6541 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
     6601 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     6661 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     6721 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     6781 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     6841 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     6901 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     6961 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     7021 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     7081 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     7141 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     7201 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     7261 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     7321 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     7381 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     7441 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     7501 gcataataag ccggtctcgg ttttagagct agaaatagca agttaaaata aggctagtcc
     7561 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttgcaaaat tttccagatc
     7621 gatttcttct tcctctgttc ttcggcgttc aatttctggg gttttctctt cgttttctgt
     7681 aactgaaacc taaaatttga cctaaaaaaa atctcaaata atatgattca gtggttttgt
     7741 acttttcagt tagttgagtt ttgcagttcc gatgagataa accaataagc ttgaccgagt
     7801 gtacttcaag tcagtgggaa atcaataaaa tgattatttt atgaatatat ttcattgtgc
     7861 aagtagatag aaattacata tgttacataa cacacgaaat aaacaaaaaa agacaatcca
     7921 aaaacaaaca ccccaaaaaa aataatcact ttagataaac tcgtatgagg agaggcacgt
     7981 tcagtgactc gacgattccc gagcaaaaaa agtctccccg tcacacatgt agtgggtgac
     8041 gcaattatct ttaaagtaat ccttctgttg acttgtcatt gataacatcc agtcttcgtc
     8101 aggattgcaa agaattatag aagggatccc accttttatt ttcttctttt ttccatattt
     8161 agggttgaca gtgaaatcag actggcaacc tattaattgc ttccacaatg ggacgaactt
     8221 gaaggggatg tcgtcgatga tattataggt ggcgtgttca tcgtagttgg tgaaatcgat
     8281 ggtaccgttc caatagttgt gtcgtccgag acttctagcc caggtggtct ttccggtacg
     8341 agttggtccg cagatgtaga ggctggggtg tcggattcca ttccttccat tgtccttgtt
     8401 aaatcggcca tccattcaag gtcagattga gcttgttggt atgagacagg atgtatgtaa
     8461 gtataagcgt ctatgcttac atggtataga tgggtttccc tccaggagtg tagatcttcg
     8521 tggcagcgaa gatctgattc tgtgaagggc gacacatacg gttcaggttg tggagggaat
     8581 aatttgttgg ctgaatattc cagccattga agctttgttg cccattcatg agggaattct
     8641 tccttgatca tgtcaagata ttcctcctta gacgttgcag tctggataat agttctccat
     8701 cgtgcgtcag atttgcgagg agaaacctta tgatctcgga aatctcctct ggttttaata
     8761 tctccgtcct ttgatatgta atcaaggact tgtttagagt ttctagctgg ctggatatta
     8821 gggtgatttc cttcaaaatc gaaaaaagaa ggatccctaa tacaaggttt tttatcaagc
     8881 tggagaagag catgatagtg ggtagtgcca tcttgatgaa gctcagaagc aacaccaagg
     8941 aagaaaataa gaaaaggtgt gagtttctcc cagagaaact ggaataaatc atctctttga
     9001 gatgagcact tgggataggt aaggaaaaca tatttagatt ggagtctgaa gttcttacta
     9061 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
     9121 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
     9181 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
     9241 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
     9301 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
     9361 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
     9421 ctcatagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     9481 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     9541 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     9601 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     9661 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     9721 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     9781 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     9841 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     9901 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     9961 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
    10021 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
    10081 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
    10141 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
    10201 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
    10261 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
    10321 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
    10381 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
    10441 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
    10501 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
    10561 cccgcgcacg gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
    10621 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
    10681 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
    10741 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
    10801 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
    10861 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
    10921 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
    10981 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
    11041 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
    11101 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
    11161 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
    11221 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
    11281 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
    11341 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
    11401 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
    11461 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
    11521 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
    11581 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
    11641 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
    11701 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
    11761 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
    11821 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
    11881 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
    11941 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
    12001 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
    12061 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
    12121 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
    12181 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
    12241 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
    12301 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
    12361 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
    12421 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
    12481 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
    12541 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
    12601 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
    12661 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
    12721 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
    12781 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
    12841 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
    12901 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct
    12961 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
    13021 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
    13081 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
    13141 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
    13201 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
    13261 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
    13321 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
    13381 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga aacggtcaca gcttgtctgt
    13441 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    13501 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    13561 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    13621 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    13681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    13741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    13801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    13861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    14041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    14101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    14161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    14221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    14281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    14341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    14401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    14461 gaacgaaaac tcacgttaag ggattttggt catgcattct agggaaggtg cgaacaagtc
    14521 cctgatatga gatcatgttt gtcatctgga gccatagaac agggttcatc atgagtcatc
    14581 aacttacctt cgccgacagt gaattcagca gtaagcgccg tcagaccaga aaagagattt
    14641 tcttgtcccg catggagcag attctgccat ggcaaaacat ggtggaagtc atcgagccgt
    14701 tttaccccaa ggctggtaat ggccggcgac cttatccgct ggaaaccatg ctacgcattc
    14761 actgcatgca gcattggtac aacctgagcg atggcgcgat ggaagatgct ctgtacgaaa
    14821 tcgcctccat gcgtctgttt gcccggttat ccctggatag cgccttgccg gaccgcacca
    14881 ccatcatgaa tttccgccac ctgctggagc agcatcaact ggcccgccaa ttgttcaaga
    14941 ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac tcaaggcacc ttggtcgatg
    15001 ccaccatcat tgaggcaccc agctcgacca agaacaaaga gcagcaacgc gatccggaga
    15061 tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac attggtgtcg
    15121 atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag catgacctca
    15181 atcagctggg taatctgctg catggagagg agcaatttgt ctcagccgat gccggctacc
    15241 aaggggcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg atcgccgagc
    15301 gccccggcaa ggtaagaacc ttgaaacagc atccacgcaa gaacaaaacg gccatcaaca
    15361 tcgaatacat gaaagccagc atccgggcca gggtggagca cccatttcgc atcatcaagc
    15421 gacagttcgg cttcgtgaaa gccagataca aggggttgct gaaaaacgat aaccaactgg
    15481 cgatgttatt cacgctggcc aacctgtttc gggcggacca aatgatacgt cagtgggaga
    15541 gatctcacta aaaactgggg ataacgcctt aaatggcgaa gaaacggtct aaataggctg
    15601 attcaaggca tttacgggag aaaaaatcgg ctcaaacatg aagaaatgaa atgactgagt
    15661 cagccgagaa gaatttcccc gcttattcgc accttcccta ggtactaaaa caattcatcc
    15721 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
    15781 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
    15841 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
    15901 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
    15961 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
    16021 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
    16081 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
    16141 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
    16201 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
    16261 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
    16321 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
    16381 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
    16441 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
    16501 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    16561 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    16621 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    16681 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
    16741 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
    16801 catgagcaaa gtctgc
//
