LOCUS       sequence-224255-       12103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express Raptor-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, 
            Moldavski O, Hurley JH, Zoncu R
  TITLE     A nutrient-induced affinity switch controls mTORC1 activation by its
            Rag GTPase-Ragulator lysosomal scaffold.
  JOURNAL   Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 
            10.1038/s41556-018-0148-6. Epub 2018 Jul 30.
  PUBMED    30061680
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0148-6"; journalName: "Nat Cell Biol"; date: 
            "2018-09"; volume: "20"; issue: "9"; pages: "1052-1063"
COMMENT     Sequence Label: pLJM1 Raptor GFP
FEATURES             Location/Qualifiers
     source          1..12103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2890..2910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3199..3402
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3353..3373
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3431..3440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7487..7496
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7493..7495,7496..7498,7499..8212)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7493..7495,7496..7498,7499..8209)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7538..7559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7799..7818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8146..8167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8254..8371
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8420..8930
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8849..8867
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8952..9551
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8952..8971)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9448..9468
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9679..9912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9984..10105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10021..10040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10075..10094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10145..10280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10300..10319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10301..10319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10329..10346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10329..10345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10338..10360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10487..10942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10574..10593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10784..10805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10968..11072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11073..11141,11142..11933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11291..11310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc
     2881 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2941 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     3001 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     3061 gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3181 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3241 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3301 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3361 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     3421 atccgctagc gccaccatgg agtccgaaat gctgcaatcg cctcttctgg gcctggggga
     3481 ggaagatgag gctgatctta cagactggaa cctacctttg gcttttatga aaaagaggca
     3541 ctgtgagaaa attgaaggct ccaaatcctt agctcagagc tggaggatga aggatcggat
     3601 gaagacagtc agtgttgcct tagttttgtg cctgaatgtt ggtgtggacc ctcccgatgt
     3661 ggtgaagacc acgccctgtg cacgcttgga atgctggatc gatcctctgt cgatgggtcc
     3721 tcagaaagct ctggaaacca tcggtgcaaa tttacagaag cagtacgaga actggcagcc
     3781 aagggcccgg tacaagcaga gccttgaccc aactgtggat gaagtcaaga agctctgcac
     3841 gtccttacgt cgcaacgcca aggaggagcg agtcctcttt cactacaatg gccacggggt
     3901 gccccggccc acagtcaacg gggaggtctg ggtcttcaac aagaactaca cgcagtacat
     3961 ccctctgtcc atatatgacc tgcagacgtg gatgggcagc ccgtcgatct tcgtctacga
     4021 ctgctccaat gctggcttga tcgtcaagtc cttcaagcag ttcgcactac agcgggagca
     4081 ggagctggag gtagctgcaa tcaacccaaa tcaccctctt gctcagatgc ctttgcctcc
     4141 gtcgatgaaa aactgcatcc agctggcagc ctgcgaggcc accgagctgc tgcccatgat
     4201 ccccgacctc ccggctgacc tattcacctc ctgcctcacc acccccatca agatcgccct
     4261 gcgctggttt tgcatgcaga aatgtgtcag tctggtgcct ggcgtcacac tggatttgat
     4321 agaaaagatc cctggccgcc tgaacgacag gaggacgccc ctgggtgaac tgaactggat
     4381 cttcacagcc atcacagaca ccatcgcgtg gaacgtgctc ccccgggatc tcttccaaaa
     4441 gctcttcaga caggacttgc tggtggctag tctgtttcga aattttttat tggcggaaag
     4501 gattatgagg tcgtataact gcactcccgt cagcagcccg cgtctgccgc ccacgtacat
     4561 gcacgccatg tggcaagcct gggacctggc tgttgacatc tgtctgtctc agctgccgac
     4621 gatcatcgag gaaggcactg cgtttcggca cagcccgttc ttcgccgagc agctgaccgc
     4681 attccaggtg tggctcacca tgggcgtgga gaaccgaaac ccacccgaac agctgcccat
     4741 cgtcctgcag gtgctgttaa gccaagtgca ccggctgaga gcattggact tgcttggaag
     4801 atttttggac ctgggtccct gggcagtgag cctggccttg tctgtcggca tcttccccta
     4861 cgtgctgaag ctgctccaga gctcggcccg agagctgcgg ccacttctcg ttttcatctg
     4921 ggccaagatc ctcgcagtgg acagctcgtg ccaggcggac ctcgtgaagg acaacggcca
     4981 caagtacttc ctgtcggtcc tggcggaccc ctacatgcca gctgaacacc ggaccatgac
     5041 ggctttcatt ctcgccgtga tcgtcaacag ctatcacacg gggcaggaag cctgccttca
     5101 gggaaacctc attgccatct gcctggagca gctcaacgac ccgcacccct tgctgcgcca
     5161 gtgggtggcc atctgcctcg gcaggatctg gcagaacttc gactcggcga ggtggtgcgg
     5221 cgtgagggac agcgctcatg agaagctcta cagcctcctc tccgacccca ttcccgaggt
     5281 ccgctgcgca gcggtcttcg cccttggcac gttcgtgggc aactctgcag agaggacgga
     5341 ccactccacc accatcgacc acaacgtggc catgatgctg gcccagctgg tcagcgacgg
     5401 gagccccatg gtccggaagg agctggtggt ggctctgagt catcttgtgg ttcagtatga
     5461 aagcaatttc tgcaccgtgg ccctgcagtt catagaagag gaaaagaact acgccttgcc
     5521 ttctccagca accacagagg gagggagttt gaccccagtg cgagacagcc cgtgcacccc
     5581 cagacttcgt tctgtgagct cctatggaaa catccgtgct gtcgccacag ccaggagcct
     5641 caacaaatct ttgcagaacc tgagtttgac agaggaatct ggtggcgcgg tggcgttctc
     5701 ccccggaaac ctcagcacca gcagcagcgc cagcagcacc ctgggcagcc ccgagaatga
     5761 ggagcatatc ctgtccttcg agaccatcga caagatgcgc cgcgccagct cctactcctc
     5821 cctcaactcc ctcatcggag tttcctttaa cagtgtttac actcagattt ggagagtcct
     5881 gctgcacctg gctgctgacc cctatccaga ggtctcggac gtggccatga aagtactcaa
     5941 cagcatcgcc tacaaggcca ccgtgaacgc ccggccgcag cgcgtcctgg acacctcctc
     6001 cctcacgcag tcggcccccg ccagccccac caacaagggc gtgcacatcc accaggcggg
     6061 gggctcccct ccggcgtcca gcaccagcag ctccagcctg accaacgatg tggccaagca
     6121 gccggtcagc cgagacttgc cttctggccg gccgggcacc acaggccccg ctggggcgca
     6181 gtacacccct cactcccacc agttcccccg gacacggaag atgttcgaca agggcccaga
     6241 gcagactgcg gacgacgcgg acgatgctgc tggacacaaa agtttcatct ccgccacggt
     6301 gcagacgggg ttctgcgact ggagcgcccg ctattttgcc cagcccgtca tgaagatccc
     6361 agaagagcac gacctggaga gtcagatccg caaggagcgg gagtggcggt tcctgcgaaa
     6421 cagccgtgtc aggaggcagg cccagcaagt cattcagaag ggcattacga gattggacga
     6481 ccaaatattt ctgaacagga accccggcgt cccctctgtg gtgaaattcc accccttcac
     6541 gccgtgcatc gctgtagccg acaaggacag catctgcttt tgggactggg agaaagggga
     6601 gaagctggat tatttccaca atgggaaccc tcggtacacg agggtcactg ccatggagta
     6661 tctgaatggc caggactgct cgcttctgct gacggccaca gacgatggtg ccatcagggt
     6721 ctggaagaat tttgctgatt tggaaaagaa cccagagatg gtgaccgcgt ggcaggggct
     6781 ctcggacatg ctgccaacga cgcgaggagc tgggatggtg gtggactggg agcaggagac
     6841 cggcctcctc atgagctcag gagacgtgcg gatcgtccgg atctgggaca cagaccgtga
     6901 gatgaaggtg caggacatcc ctacgggcgc agacagctgt gtgacgagtc tgtcctgtga
     6961 ttcccaccgc tcactcatcg tggctggcct cggtgacggc tccatccgcg tctacgacag
     7021 aaggatggca ctcagcgaat gccgcgtcat gacgtaccgg gagcacacag cctgggtggt
     7081 gaaggcctcc ctgcagaagc gtcccgacgg ccacatcgtg agtgtgagcg tcaatggaga
     7141 tgtgcgcatc tttgatcccc ggatgcctga gtcggtaaat gtgcttcaga tcgtgaaggg
     7201 gctgacggcc ctggacatcc acccccaggc ggacctgatc gcatgtggct ccgtcaatca
     7261 gttcaccgcc atctacaaca gcagcggaga gctcatcaac aacatcaagt actacgacgg
     7321 cttcatgggc cagcgggtcg gcgccatcag ctgcctggcc ttccacccgc actggcctca
     7381 cctggccgtg ggaagcaacg actactacat ctccgtgtac tcggtggaga agcgtgtcag
     7441 agggatactg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     7501 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     7561 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     7621 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     7681 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7741 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7801 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7861 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7921 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7981 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     8041 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     8101 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     8161 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattctc
     8221 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8281 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8341 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8401 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     8461 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     8521 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     8581 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     8641 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     8701 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     8761 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     8821 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     8881 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
     8941 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     9001 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9061 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     9121 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     9181 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     9241 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     9301 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     9361 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     9421 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     9481 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     9541 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     9601 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9901 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9961 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    10021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10081 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    10141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10201 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10261 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    10321 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    10381 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    10441 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    10501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    10561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10921 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    12001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
