LOCUS       sequence-224347-        5565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse full-length SynCAM 1, CAG promoter suited for 
            neuronal expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
  TITLE     SynCAMs organize synapses through heterophilic adhesion.
  JOURNAL   J Neurosci. 2007 Nov 14;27(46):12516-30. doi: 
            10.1523/JNEUROSCI.2739-07.2007.
  PUBMED    18003830
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2739-07"; journalName: "J Neurosci"; date: 
            "2007-11-14- 14"; volume: "27"; issue: "46"; pages: "12516-30"
COMMENT     Sequence Label: pCAGGS mSynCAM1
FEATURES             Location/Qualifiers
     source          1..5565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..45
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     107..126
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1235..1261
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHYHHHHH"
     primer_bind     complement(2085..2104)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2150..2205
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2204..2223)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2566..2582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2566..2582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2579..2601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2590..2606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2614..2620,2621..2638,2639..2644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2659..2680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2739..2934
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2785..2920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2940..3074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2977..2996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3031..3050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3142..3159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3393..3412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4072..4863,4864..4932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4695..4714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4933..5037)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5068..5447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
       61 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
      121 attgtgctgt ctcatcattt tggcaaagaa ttcctcgagc actgttggcc tactggagtc
      181 tgaggcaggt gcccgacatg gcgagtgctg tgctgccgag cggatcccag tgtgcggcgg
      241 cagcggctgt ggcggcggcg gcggcgcctc cagggctccg gctccggctc ctgctgttgc
      301 tcctttcggc cgcggcactg atccccacag gtgatggaca gaatctgttt actaaagacg
      361 tgacagtgat tgaaggagaa gtggcaacca tcagctgcca ggtcaataag agtgacgact
      421 cagtgatcca gctcctgaac cccaacaggc agaccattta cttcagggac ttcaggcctt
      481 tgaaggacag caggtttcag ctgctgaatt tttctagcag tgaactcaaa gtgtcactga
      541 cgaatgtctc aatctcggat gaagggagat acttctgcca gctctacacg gaccccccac
      601 aggagagtta caccaccatc acagtcctgg ttcctccacg taacttgatg atcgatatcc
      661 agaaagacac ggcagttgaa ggggaggaga ttgaagtcaa ctgtactgcc atggccagca
      721 agccagcgac gaccatcagg tggttcaaag ggaacaagga actcaaaggc aaatcagagg
      781 tggaggagtg gtcggacatg tacactgtga ccagtcagct gatgctgaag gtgcacaagg
      841 aggacgacgg ggtcccggtg atctgccagg tggagcaccc tgcggtcact ggaaacctgc
      901 agacccagcg ctatctagaa gtgcagtata aaccgcaagt gcatatccag atgacttacc
      961 ctctgcaagg cctaacccgg gaaggggatg catttgagtt aacgtgtgaa gccatcggga
     1021 agccccagcc tgtgatggta acttgggtga gagtcgatga tgaaatgcct caacatgccg
     1081 tactgtctgg gccaaacctg ttcatcaata acctaaacaa aacagataac ggtacttacc
     1141 gctgtgaggc ttccaacata gtgggaaagg ctcattcgga ctatatgctg tatgtatacg
     1201 atccccccac aactatccct cctcccacaa caaccaccac cactaccacc accaccacca
     1261 cctccatcct taccatcatc acagattctc gagcaggtga agaggggacc attggggcag
     1321 tggaccacgc agtgattggt ggcgtcgtag ccgtggtggt gtttgccatg ctatgcttgc
     1381 tcatcattct gggccgctat tttgccagac ataaaggtac atacttcact catgaagcca
     1441 aaggagccga tgacgcagca gacgcagaca cagctataat caatgcagaa ggaggacaga
     1501 acaactccga agaaaagaaa gagtacttca tctagatcag cctttttgtt ccaatgaggt
     1561 gtccaactgg cctgtttaga tgataaagag acagtgatac tggaactttc gagaagctcg
     1621 tgtggttttt tgttttgttt tgttttttta tgagtgggtg gagagatgcg agactgggaa
     1681 ggcttgggat ttgcaatgta caaacaaaaa caaagaatgt tctttgaaag tacactctgc
     1741 tgtttgacac ctctttttaa tctggtttta atttgctttg ggttttgggt ttttttggtt
     1801 ttttgttttt ttcatttata tttcttccta ccaagtcaaa cttgggtact tggatttggt
     1861 ttcggtagat tgcagaaaat tctgtgcctt gtttttcatt cgtttgttgt gtttcttccc
     1921 tcttgcccat ttatttttcc caaaatcaaa tttgtttttt tccccctccc aaacctccca
     1981 ttttttggaa ttgacctgct ggaattcact cctcaggtgc aggctgccta tcagaaggtg
     2041 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     2101 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2161 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2221 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2281 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     2341 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2401 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2461 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2521 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     2581 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2641 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2701 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     2761 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     2881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     2941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3061 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4021 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5041 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
     5101 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5161 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5221 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5281 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5341 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5401 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     5461 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     5521 ttatttttta attattttgt gcagcgatgg gggcgggggg ggggg
//
