LOCUS       sequence-224512-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1410 GFP-TDP43 F-S
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3149..3158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5117..5141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5177..5765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5230..5250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5648..5659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5779..6278
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5814..5837)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6211..6230
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6299..6898
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6299..6318)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6795..6815
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7036..7269
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7341..7462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7378..7397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7432..7451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7502..7637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7657..7676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7658..7676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7686..7703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7686..7702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7695..7717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7844..8299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7931..7950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8141..8162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8325..8429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8430..8498,8499..9290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8648..8667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9461..9927,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatta ccaccggtgc caccatggga agtaaaggag aggaactatt
     3181 tacaggtact gtgcctatca aagtcgagct ggacggcgac gtaaacggcc acaagttcag
     3241 cgtgcgtggc gagggcgagg gcgatgccac cgagggcaag ctgaccctga agttcatctg
     3301 caccaccggc aagctgccgg tgccgtggcc gaccctcgtg accaccctga cctacggcgt
     3361 gcagtgcttc agccgctacc cggaccacat gaagcgtcac gacttcttca agtccgccat
     3421 gccggaaggc tacgtccagg agcgcaccat cgagttcaag gacgacggca cctacaagac
     3481 ccgcgcggag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcaa
     3541 cgacttcaag gaggacggca acatcctggg tcacaagctg gagtacaacc acaacagcca
     3601 caacgtccgt atcgaagccg acaagcagaa gaacggcatc aaggctaact tcaagatccg
     3661 ccacaacgtt gaggacggca gccagcagga agccgaccac aaacagcaga acaccccgat
     3721 cggcgacggc ccggtgcgcc tgccggacaa ccactatctg agcacccaga ccacgctgag
     3781 caaagacccg aacgagaagc gcgatcacat ggtcctgaag gagttcgtga cggcagccgg
     3841 tatcactaaa ggtgaagatg aaagtggatc catgtctgaa tatattcggg taaccgaaga
     3901 tgagaacgat gagcccattg aaataccatc ggaagacgat gggacggtgc tgctctccac
     3961 ggttacagcc cagtttccag gggcgtgtgg gcttcgctac aggaatccag tgtctcagtg
     4021 tatgagaggt gtccggctgg tagaaggaat tctgcatgcc ccagatgctg gctggggaaa
     4081 tctggtgtat gttgtcaact atccaaaaga taacaaaaga aaaatggatg agacagatgc
     4141 ttcatcagca gtgaaagtga aaagagcagt ccagaaaaca tccgatttaa tagtgttggg
     4201 tctcccatgg aaaacaaccg aacaggacct gaaagagtat tttagtacct ttggagaagt
     4261 tcttatggtg caggtcaaga aagatcttaa gactggtcat tcaaaggggt ttggctttgt
     4321 tcgttttacg gaatatgaaa cacaagtgaa agtaatgtca cagcgacata tgatagatgg
     4381 acgatggtgt gactgcaaac ttcctaattc taagcaaagc caagatgagc ctttgagaag
     4441 cagaaaagtg tttgtggggc gctgtacaga ggacatgact gaggatgagc tgcgggagtt
     4501 cttctctcag tacggggatg tgatggatgt cttcatcccc aagccattca gggcctttgc
     4561 ctttgttaca tttgcagatg atcagattgc gcagtctctt tgtggagagg acttgatcat
     4621 taaaggaatc agcgttcata tatccaatgc cgaacctaaa cataattcaa accggcagtt
     4681 ggaaagatcg gggcgcagtg gtggcaaccc aggcggttct ggtaatcagg gcggctcggg
     4741 aaattccaga ggcgggggtg cagggcttgg caacaatcag gggtccaata tgggcggtgg
     4801 tatgaactca ggtgccagct cgattaatcc ggccatgatg gcggcagcgc aggcggcgct
     4861 gcagtcttcc tggggtatga tgggtatgtt agcatcccaa cagaaccaga gtggcccgtc
     4921 ggggaataac caaaatcagg ggaatatgca gcgcgaaccg aatcaagctt caggtagtgg
     4981 taacaacagt tatagtggat cgaacagtgg cgccgcaatt ggatggggtt ccgcatccaa
     5041 tgcaggctca ggcagcggct cgaatggcgg ctcaggttct agtatggatt cgaagagctc
     5101 tggttgggga atgtagaccc agctttcttg tacaaagtgg ttgatcctct atccagcaca
     5161 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5221 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5281 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5341 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5401 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5461 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5521 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     5581 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5641 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5701 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5761 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5821 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5881 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     5941 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6001 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6061 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6121 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6181 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6241 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     6901 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     6961 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7021 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     7081 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7141 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7201 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7261 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7321 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7441 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7561 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7621 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7681 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8281 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9901 cggtaagcgg cagggtcgga acaggag
//
