LOCUS       sequence-22464-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi Cre
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(92..110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(117..134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(117..133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(126..148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    367..388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(403..408,409..426,427..433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    441..457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     446..468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     465..481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     465..481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    514..635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(525..544)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtttatatc ttggtggaag gacgaaacac cgatacaaag gatatcaggt gcgaacacct
       61 gatatccttt gtataaaaag gcccaattcg ccctatagtg agtcgtatta caattcactg
      121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      241 tcccaacagt tgcgcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      301 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      361 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      421 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      481 ccatgattac gccaagcttg ggcccagaca tgataagata cattgatgag tttggacaaa
      541 ccacaactag aatgcagtga aaaaaatgct ttatttgtga catttgtgat gctattgctt
      601 tatttgtaac cattataagc tgccataaac aagttaacaa caacaattgc attcatttta
      661 tgt
//
