LOCUS       sequence-224643-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fluorescent protein expression with an N-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, 
            Birtwistle MR
  TITLE     Fluorescence Multiplexing with Spectral Imaging and Combinatorics.
  JOURNAL   ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 
            10.1021/acscombsci.8b00101. Epub 2018 Oct 30.
  PUBMED    30339749
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscombsci.8b00101"; journalName: "ACS Comb Sci"; date: 
            "2018-11-12- 12"; volume: "20"; issue: "11"; pages: "653-659"
COMMENT     Sequence Label: pQLinkHD_EBFP2-mTFP1
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..759)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGTTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ctcngctatt nagcttacca ctttgtacan gaaagctggg tcaagctttt acttgtacag
       61 ctcgtccatg ccgtcggtgg agttgcgggc cacggcgctc tcgtaaacgg tcaccttgtt
      121 gtagtccttg tcgtggttca ggatctcgat gcggtggtcc acaaagtgat agtcgggcag
      181 cttcaccgcc ttcttggccc tgtagatggt cttgaagtca acgcggtggt ggccgccgcc
      241 ctccagcagc agcttgtgct tgacgtcgcc cttcagcacg ccgtcgcgca cgtacatcct
      301 ctcggtggag gcgtcccagc cggtggtctt cttctgcatc acggggccgt tgggggggaa
      361 gttctcgccc ttgaggtgta tctcgtagat gaaggagtcc tcctccatgg agatgtcgga
      421 cttcaccttc acgatgccct tgtcctcgaa ggtcatggtg cgctcccaag agtagccctc
      481 ggggaaggac tgcttgaagt agttggggat gtcgtcgggg tacttggtga aggccctgtt
      541 gccgtaggcg aacgcggtgg tcagaatgtc gtaggagaag ggcagggggg ctccctcctt
      601 cacctccagg ttgatggtgt tggtgccgtc gtagggcttg ccctcgccct cgccctcgat
      661 cacgaaggcg tggccattca cgttgccctc catcttcagc ttgatcttca tgtcgggctt
      721 gattacgccc attgtggtcc cctcgccctt gctcaccatt cctaggcccc cacctccggc
      781 accggtcttg tacagctcgt ccatgccgag agtgatcccg gcggcggtgc ggaactccag
      841 caggaccatg tgatcgcgct tctcgttggg gtctttgctc ancacggact gggtgctcag
      901 gtagtggctg tcgggcancn ncacggggcc gtccccnaat gggggtgttc tgctggtatt
      961 ggtcggnnan ntncncgctg cccncc
//
