LOCUS       sequence-224749-        9603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to Rab27b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Rab27b
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1427..1447)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1455..1471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1487..1621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1511..1530)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1565..1584
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2060..2247,2248..2259)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2293..2309)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2293..2309)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2346..2366)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2346..2364)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2385..2401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2398..2420)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2409..2425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2433..2439,2440..2457,2458..2463))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2478..2499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2616..2633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3546..4337,4338..4406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4169..4188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4407..4511)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4674..4695)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5044..5055,5056..5218)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5591..5607
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5627..5647
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgttcagc tccagcagca ctatgaccga
      781 tggagattac gattatctga tcaaactcct ggctttgggg gactccgggg tcggcaagac
      841 cactttcctc taccgataca cagataacaa gttcaacccc aaattcatca ccaccgtggg
      901 catcgacttc agggaaaaga gagtggttta tacgactaac agccccaatg ggacgacggg
      961 gaaaaccttt aaggttcacc tgcagctgtg ggacactgcg ggacaggaga ggttcaggag
     1021 tttgaccact gcattcttca gggatgccat gggttttctg ctgatgtttg atctgaccag
     1081 ccagcagagc tttctcaacg tcaggaactg gatgagtcag ctccaggcta atgcatattg
     1141 tgagaatcca gatatcgtgc tggtgggaaa taaagcagat ctagccgacc agagagaagt
     1201 tcaggagaaa caagcgaaag aactcgcaga caaatacggc atcccgtact tcgagacgag
     1261 tgcagcgacg ggctctgagg tggacaaggc tgtggtgacg ctgctggatc tggtgatgaa
     1321 gcggatggag cagtgtgtgg agaagcccag cgcagacgca aacaccagcg acggcagcag
     1381 caaactgagc gccgcacctg cccaacagaa gaagtgtgca tgctgacaac tttattatac
     1441 atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat
     1501 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1561 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1621 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     1681 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     1741 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     1801 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     1861 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
     1921 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag
     1981 gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat
     2041 agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa
     2101 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     2161 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     2221 agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca
     2281 gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     2341 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     2401 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2461 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2521 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2581 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2641 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3421 gaaaactcac gttaaggcat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4441 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4501 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4561 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     4621 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     4681 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     4741 atcagggcga tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac
     4801 gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt
     4861 ataggaatgg agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt
     4921 tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat
     4981 ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct
     5041 tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg
     5101 aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa
     5161 aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag
     5221 aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca
     5281 aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc
     5341 taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg
     5401 attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt
     5461 gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag
     5521 gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggccagatg
     5581 ggccctcgag caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga
     5641 aaagttggca cgcgcagatt ccgtgtgggc ctggtaatat cttaccattt taagctattt
     5701 taagattagg gaaaaaaatt aaataaaact actttaaaat tactttcctt aaaaagcaac
     5761 atatttattt agtttttgtt taactggctc aagattgtaa tattttactt ttacatgttt
     5821 ttttaagtac ctaaatgtgt tcaaaagtaa agtatgttat tgtttttttt ttttaatgtt
     5881 agctaagctt taaattgtaa tatattactt ttaaaagtga ctttttaaaa atgtaaataa
     5941 aaaagtaact ataaatgtgt aatgcatgtt atttctgtgg gtttttaatt ttcatctcaa
     6001 ctgggcttac aattttaaca taataaaaat caaatcaaat caaatcaggc ccttatttgt
     6061 cacatacact cttgtatact gaaataaact aaatgttgag aaaaatttaa atacaccaaa
     6121 ataacgcata ccttttttgt aattacctaa gtaaaacatt tagttccttt tttaagtgac
     6181 tcaagattgt aatctattaa ttgtaaaaga taaaactagt gatcaatctg acgtgcttct
     6241 atgcagcctg tgagattaat tgtgatgttt aattattaaa caatttaaat ggatcaaaca
     6301 tttcacatgt gcttattgtg tccaacaaaa ccagcagaag cacttaaaaa tattgcatga
     6361 caacatgagt ttttaatgta aattatatat acttcctaaa cgaagttaat catatttcta
     6421 aaataataat gtcattgaaa tatttaaata aatgataagc tttagttgat tcaacataaa
     6481 attgaaagca ataaggactt ttttttttta cagcggtgaa gcaaaagcat gtaataatca
     6541 tataatataa taaataaata aataaataat ggctcttggt gcaaatatga acactttaaa
     6601 taaatataga ataaatatga tcaataagtc ataacgatgt gttttatgtt gattataatt
     6661 taaacatgtt tctaacgcaa gttttaaaaa ctgttttaaa gtattatttt aagttataag
     6721 atcaatttga ctcaacattt tgtagtgaaa atatgttgtg taaaattaat ataatataca
     6781 ctataaaaat gcagggttcc acacaataca ttaatgttgt cccaacacaa atcgattaag
     6841 ttaacaaata tatgtggatt gaacataaaa aattaagttg tcccaatgaa atctcaagaa
     6901 ttgtgttgtt tcagctcatt ttaaataagt agttttaaca agtaaaaaat aacatatttt
     6961 tgagtgtgta tatatatata ttacaaaata taataatata aaatgtaaaa gatataaacc
     7021 atgctacata tatttaagag ctattttgca cattactttt ttgcaagcca ttttagtgtt
     7081 ttttttacaa atatatatta gctaaatata caatatctta aatatacaat taatcagaca
     7141 tgcaattaat gcatttaacc tacataaaaa tacatgatca acatatgttt tatgctgatt
     7201 acaactaaat caaacgtaat gaaattaatt tataaaagcg atttaaatat gtcaatattt
     7261 taagttaaaa tcacttgaaa cctactataa atgaacttaa attaaaaaaa tctcctttaa
     7321 ttccaatgca acagtgttgt tttttcagct gaggaccccc ggtgcagatg gttggccccc
     7381 ggcccgctat tctaaggaag cctttcttaa ttttagggcg gcgctgcatg cgctcagatc
     7441 agattcacgg gtggagaggg ttattttctc ttttttattt tttcttgcct gctcatattt
     7501 gggcctgtgt gaaccccagg ctccgcctcg gcccccagga gccgctctta tttaaagggg
     7561 atcctcgcct tcagtcttcg tcccagtgga agcatcagtc ttgtgataac aacttggtga
     7621 gtcctacaga ccagcgctcg actctaggct ttaaatgcat tgatttctct ggattagcta
     7681 ccgggagagt tttaactttg cttattagtc ttgtgctcga actgctaggt ttaaagagtg
     7741 ttttctctaa tgcatgcatg aagcgacagc tgaaaaccag aaatagagcg tttgtcctcc
     7801 tgttaaactc tagcctttct ttggcatttg tcagtccgag tcttcagctg ccggcaggag
     7861 attgggtttc agtaacactt gctgagcaca aactacacgt gctttcttca gatttaattc
     7921 attattaatc attctagatt taaatgatgt tcttactcat tgctgattat tttagccttg
     7981 cggagcagat taaatgagca cgtgtttcat taagattgat ttaaatataa ttaatatttc
     8041 accactgtag ttttaaaatt gccttctaag tcatggttga tttttaatag gtatcggctc
     8101 taattagatt actattttgg tttttaaatg ttatttctat ttcatttgta ggctcgtttt
     8161 taatgtgatt ttattagatt ctagttttaa aatcatctct tagccttaat gagcagactg
     8221 aatgaacatg tttcattaag attaacttca ttataatgaa ttatttaatc attctagtgg
     8281 gttataaatc agctctattt aaattaattg tcgtgtttat ttctatttaa tttttgatta
     8341 ttttttcagg gtcgttttta tgtgattttt tcccgtttct aattttaaac taattgtttg
     8401 atttttatat gattcgactc tttttaaatg aattgttttg ctttttttat tgagcatata
     8461 tatattatac aatttcaatc aacaattgtt gtgcttttta aatgttgttt ctatttcatt
     8521 tctagggtcg attttattcg attctagttt taaaatcgtg atttttaact tctaacctta
     8581 ctgagcagac tgaatgaaca tgtttcatca agattaactt cattatagtt aattattgaa
     8641 tcgtgctagt ggtttataaa tcagctctat ttaaattaat catcatcatt atttctattt
     8701 aatttttatt atttctttag gattattttt atgtgatttt tttcacgttc tagttttaaa
     8761 ctcaatgctc attactgctc acccttgctg agcagtcttt cgttaagatt aaattaatta
     8821 ataaccattc tagttttaaa acagtgtctt aaggcatagc tgatatttta caagtttact
     8881 atttaatata tctcctttct ttttttaggg tcgatttcat ctgatttgat taatttttat
     8941 tatcttcact aagctttttg ttctaggttt tattctcagt attattactg ttatatttga
     9001 actgaatttc tcctcatagt ttattcattt tatttattta ttttttgttt attcgctatt
     9061 ttgtatttta gtttttaatt agaaatttta ttagaatcgt tttgaactag tttttatgca
     9121 ctctaacatt aatattaaat cataactatt atttttatta taatttattt gtgtttatct
     9181 ttttgtgtag ctttcatgtg tttttatttt agttttatta tgtttgcatt gaattttttg
     9241 gactagttta tataacgata tacagaaaag ccatgacaac aaatgttttt ataataccta
     9301 ttaagtataa gctaatcaga tttccactat tctgttcaaa gttaacatta ttagccattc
     9361 tgatggatca tttgacattt gtcatatgtc tttatgaaca ctgctctttc agatgccact
     9421 tttaaagctg ttttttacag taaatgaacg tttattatta tgacattatt tcctaaggcc
     9481 tctagagtac agcaggatgt ggcgtgtgca ttaccgtgta attatgctga ctgtaaatgt
     9541 gcatttatag actgtcagca tgtctgactg tgcgctgttg tgattggtcc tgcacagacc
     9601 aag
//
