LOCUS       sequence-224888-        9566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  broad-host range plasmid to constitutively express fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE165
FEATURES             Location/Qualifiers
     source          1..9566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3147..3169
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3161..3178
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3188..3207
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3249..3335
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3432..3518
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3615..3701
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3796..3882
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3984..5183)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6316..7011
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6449..6467)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6717..6736
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      7028..7122
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7166..7825
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7232..7251)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      7890..7976
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8315..9109)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8426..8445)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9036..9055
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctctcagcga tcggctcgtt gccctgcgcc gctccaaagc
     4021 ccgcgacgca gcgccggcag gcagagcaag tagagggcag cgcctgcaat ccatgcccac
     4081 ccgttccacg ttgttataga agccgcatag atcgccgtga agaggagggg tccgacgatc
     4141 gaggtcaggc tggtgagcgc cgccagtgag ccttgcagct gcccctgacg ttcctcatcc
     4201 acctgcctgg acaacattgc ttgcagcgcc ggcattccga tgccacccga agcaagcagg
     4261 accatgatcg ggaacgccat ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg
     4321 ccgtcggcaa tcattccgag catgagtgcc cgcctttcgc cgagccgggc ggctacaggg
     4381 ccggtgatca ttgcctgggc gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg
     4441 atcgtggtcg cgtcccagtg aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc
     4501 acctgtccga caagttgcat gatgaagaag accgccatca gggcggcgac gacggtcatg
     4561 ccccgggccc accggaacga agcgagcggg ttgagagcct cccggcgtaa cggccggcgt
     4621 tcgcctttgt gcgactccgg caaaaggaaa cagcccgtca ggaaattgag gccgttcaag
     4681 gctgccgcgg cgaagaacgg agcgtggggg gagaaaccgc ccatcagccc accgagcaca
     4741 ggtcccgcga ccatcccgaa cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc
     4801 tcatcgccat cagtgatatc ggcaatataa gcgccggcta ccgccccagt cgccccggtg
     4861 atgccggcca cgatccgccc gatatagaga acccaaagga aaggcgctgt cgccatgatg
     4921 gcgtagtcga cagtggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga
     4981 tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc
     5041 agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc
     5101 agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg
     5161 atcaggggta tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga
     5221 acgcgcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag
     5281 tgtcaatcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga
     5341 acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc
     5401 agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
     5461 ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
     5521 ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
     5581 gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
     5641 gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
     5701 gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
     5761 gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
     5821 tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
     5881 tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gctaagaaac
     5941 cattattatc atgaagctcg cacgctgccg caagcactca gggcgcaagg gctgctaaag
     6001 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     6061 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     6121 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     6181 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     6241 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatacta
     6301 gtggaggaag aaaaaatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     6361 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     6421 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     6481 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcatcaag
     6541 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     6601 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     6661 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     6721 gtaatgcaga agaagacaat gggctgggaa gcatccaccg agcggttgta ccccgaggac
     6781 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg
     6841 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     6901 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     6961 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaattagct
     7021 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     7081 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag
     7141 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     7201 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     7261 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     7321 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     7381 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     7441 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     7501 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     7561 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     7621 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     7681 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     7741 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     7801 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     7861 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
     7921 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     7981 cgctaggagc ttgcggccgc cagtgtgatg gatatctgca taaaaactgt tgtaattcat
     8041 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     8101 gcatcagcac cttgtcgcct tgcgtataat atttgcccat gggggtgggc gaagaactcc
     8161 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     8221 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     8281 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     8341 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     8401 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     8461 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     8521 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     8581 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     8641 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     8701 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     8761 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     8821 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     8881 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     8941 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     9001 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     9061 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     9121 ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     9181 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     9241 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
     9301 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     9361 tgacattcat cccaggtggc acttttcggg gaaatgtgcg cgcccgcgtt cctgctggcg
     9421 ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc agcagctttt cgcccacggc
     9481 cttgatgatc gcggcggcct tggcctgcat atcccgattc aacggcccca gggcgtccag
     9541 aacgggcttc aggcgctccc gaaggt
//
