LOCUS       sequence-225116-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  single guide RNA targeting UBE2C; guide 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pXPR_BRD003 UBE2C guide 1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..444
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     196..216
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     367..386
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        466..541
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    597..714
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        853..2031
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1084..2022
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1979..1999
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2044..2643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2044..2063)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2540..2560
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2659..3247
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2712..2732)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3130..3141)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3319..3552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3630..3751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3667..3686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3721..3740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3791..3926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3946..3965)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3947..3965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3975..3992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3975..3991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3984..4006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4133..4588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4220..4239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4430..4451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4614..4718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4719..4787,4788..5579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4937..4956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5750..6338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6239..6258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6492..6509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6626..6647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6662..6667,6668..6685,6686..6692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6700..6716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6705..6727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6724..6740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6724..6740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6759..6779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6761..6779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6807..7033
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7034..7214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7261..7386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7879..8112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(8297..8323,1..18)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
       61 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
      121 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
      181 ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac
      241 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      301 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      361 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      421 atcttgtgga aaggacgaaa caccgcggct cagctccttt acggggtttt agagctagaa
      481 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      541 cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt
      601 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      661 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      721 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga
      781 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
      841 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      901 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
      961 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     1021 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     1081 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     1141 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     1201 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     1261 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     1321 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     1381 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     1441 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1501 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1561 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1621 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1681 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1741 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1801 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1861 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1921 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1981 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg acgtacggcc
     2041 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     2101 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     2161 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     2221 gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg
     2281 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     2341 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     2401 gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg
     2461 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     2521 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     2581 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     2641 tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2701 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2761 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2821 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2881 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2941 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3001 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3061 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3121 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3181 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3241 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3301 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3361 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3541 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3601 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3661 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3721 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3901 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3961 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4021 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4081 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4141 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4201 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4261 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4321 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4381 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4441 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4501 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4561 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4741 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4801 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4861 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4921 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4981 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5041 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5101 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5161 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5221 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5281 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5341 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5401 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5461 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5521 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5581 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5881 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6361 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6481 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6541 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6601 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6661 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6721 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6781 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6841 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6901 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6961 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7021 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7201 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7261 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7321 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7381 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7441 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7501 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7561 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7621 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7681 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7741 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7801 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7861 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7921 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7981 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8041 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8101 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8161 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8221 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8281 gcaaaaccag caagaaaaga atgaacaaga attattggaa tta
//
