LOCUS       sequence-225508-       11632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a combination of proteases and their reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Gao XJ, Chong LS, Kim MS, Elowitz MB
  TITLE     Programmable protein circuits in living cells.
  JOURNAL   Science. 2018 Sep 21;361(6408):1252-1258. doi: 
            10.1126/science.aat5062.
  PUBMED    30237357
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-09-21- 21"; volume: "361"; 
            issue: "6408"; pages: "1252-1258"
COMMENT     Sequence Label: PB-CMV-TO-Casp-rastvTEVca-teTVMVd1
FEATURES             Location/Qualifiers
     source          1..11632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1409..1788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1789..1992
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1943..1963
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1994..2012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2015..2033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2043..2067
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2159..2168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2693..2713
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3032..3052
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     misc_feature    3142..3709
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3159..3711
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3987..4007
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             4377..4430
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4800..4820
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     misc_feature    5414..5981
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5431..5983
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6370..6390
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             6511..6531
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(7012..7029)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7018..7225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7258..7278)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7263..7592
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7443..7578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7505..7524
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7640..7687
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7706..8080
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(8140..8160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8202..8323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8239..8258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8293..8312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9086..9120
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9611..9627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9611..9627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9624..9646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9635..9651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9659..9665,9666..9683,9684..9689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9704..9725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9842..9859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10013..10601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10093..10112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10772..11563,11564..11632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11395..11414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtaggcgttt tgcgctgctt
     1381 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     1441 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1501 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1561 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1621 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1681 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1741 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1801 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1861 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1921 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1981 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
     2041 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2101 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagcttcgc
     2161 caccatggga tccgaaaaca ctgagaacag cgtcgatagc aagtccatta agaacctcga
     2221 acctaaaatc atacatggca gtgagagcat ggatagcggc ataagccttg acaactccta
     2281 taaaatggac tacccggaga tgggtctgtg cataataatc aacaacaaga acttccacaa
     2341 atccacgggg atgacaagta ggtctggaac cgatgtcgat gcggcaaact tgcgcgagac
     2401 ctttagaaac ctcaagtacg aggtacgcaa taaaaacgat ctcactcgcg aggaaatagt
     2461 agagctgatg cgagatgtca gtaaggaaga tcattccaag agatcctcct tcgtctgcgt
     2521 cttgttgagt cacggagagg aagggatcat attcggtacg aacggcccag tggatttgaa
     2581 aaagataacc aatttcttta gaggtgaccg gtgccgctct ctcacaggaa agccaaaact
     2641 ctttattatc caagcatgta ggggtacgga actggattgt ggaatcgaaa ctgagaactt
     2701 gtacttccag tccggggtag atgacgacat ggcctgccat aaaatccctg tcgaggccga
     2761 ttttctctac gcctactcta ctgcgcctgg gtattacagc tggagaaaca gcaaggatgg
     2821 ttcctggttt atacaaagcc tgtgtgctat gctcaaacag tatgcggata agctggagtt
     2881 catgcatatc cttaccagag taaatcgcaa agtggccaca gagtttgagt cattttcttt
     2941 tgacgcaaca tttcacgcca agaagcagat tccttgtata gtatctatgc tgacgaaaga
     3001 gctgtacttc tatcacagca ccggcggatc tgaaaccgtg cggtttcaat ccagcggcgg
     3061 tggaggacgg agcaaactga atccacccga tgagtctgga cccggttgta tgtcatgtaa
     3121 atgtgtgttg agctgaccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3181 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3241 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3301 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3361 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3421 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3481 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3541 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3601 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3661 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     3721 ccatggatcc gagtaagacg tcaaacacaa tcgccgtttt tctccccaat aaacagcgga
     3781 ccgtggtaaa cgtccgaaac gggatgagtc tccacgactg tctgatgaaa gccttgaagg
     3841 tgagggggct tcagcctgaa tgttgcgctg tctttcgcct tctccacgaa cacaagggcg
     3901 caaaggcgag gcttgactgg aatacagacg ctgcaagttt gattggggaa gaacttcagg
     3961 tcgatttcct caccggcggt ggatccgaaa ccgtgcggtt tcaatccggt ggctcaggga
     4021 gcaagagtat gagttctatg gtgtccgaca ctagctgcac gttcccctca agtgatggga
     4081 tattctggaa acactggata cagactaaag acggacagtg tggaagccca ttggtttcca
     4141 cccgagatgg ttttatcgtg ggtatccata gcgcctctaa tttcacaaac acgaacaact
     4201 atttcacttc agtgcccaaa aactttatgg agctgctcac aaaccaagaa gcgcagcagt
     4261 gggtaagcgg ttggagactt aacgctgact cagttctctg gggggggcac aaagtattca
     4321 tggtaaagcc agaggagcca ttccaaccag tcaaagaagc cacacaactt atgaacgaag
     4381 ggcggggaag cctgttgaca tgcggggatg tagaagagaa tccaggcccc ggggagagtt
     4441 tgtttaaggg gccaagggac tataacccaa tatcatccac tatttgccac ctcactaacg
     4501 agagcgatgg acatacaacc tctctctacg ggataggctt cggtcctttc atcattacca
     4561 ataagcatct gtttcgccga aacaacggta ctcttctggt tcaatctctt catggtgtct
     4621 tcaaggtgaa aaacaccact acgcttcagc aacacctgat tgatggtagg gatatgataa
     4681 ttatcagaat gccgaaagat ttcccacctt ttccacagaa gctgaaattc agggaaccgc
     4741 agagagagga gaggatttgt ttggtaacga ccaacttcca gacgggctct ggcagcagcg
     4801 agacggttag attccagagt ggaggcggtg gagctatgac ggagtataaa ttggtggtgg
     4861 taggagccgt gggcgtagga aaaagcgccc ttactatcca gctcatacag aatcattttg
     4921 tcgacgagta cgaccctaca atagaagatt catacaggaa gcaggtcgtc atcgacggag
     4981 agacctgtct cctcgacatc ctcgatactg caggccaaga ggaatatagc gcgatgcgcg
     5041 atcaatatat gaggacagga gaaggttttc tgtgtgtttt tgctattaac aatactaaga
     5101 gttttgagga catccatcag tatagggagc agattaaaag ggttaaagac tctgatgacg
     5161 ttcctatggt tctcgttggg aacaagtgcg acctggctgc ccgcactgtt gagagtcgac
     5221 aagcacaaga cttggcaaga tcctatggta tcccgtacat agagacttcc gctaaaactc
     5281 gacaaggtgt ggaagacgcc ttttatacgc tggtacgcga gatcagacaa cacaagttgc
     5341 gcaagttgaa cccccctgac gaaagtggcc caggctgcat gagttgtaaa tgcgtcctca
     5401 gctgatatcc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5461 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5521 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5581 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5641 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5701 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5761 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5821 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5881 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5941 accacgggga cgtggttttc ctttgaaaaa cacggtgata atatggccac aaccgtgtca
     6001 aaagcactcc tgaagggagt ccgcgacttt aatcctatat cagcgtgtgt atgtttgctt
     6061 gaaaactcct ctgatgggca ttcagaaagg ctttttggta ttgggtttgg tccctacata
     6121 atagcaaacc aacacctgtt tcgacgcaat aacggcgagc ttacgattaa gacgatgcac
     6181 ggggaattta aagtgaaaaa cagcacacaa ttgcaaatga aacccgtcga agggagagac
     6241 atcattgtaa taaagatggc aaaggatttc ccaccctttc cgcagaaatt gaaattccgg
     6301 caacctacta taaaggatcg agtttgtatg gtttctacga actttcagca aggctctggc
     6361 agcagcgctg agaacttgta cttccagagt ggcggaggtg gcagcgagca actcgaaaag
     6421 aagctgcaag cgttggagaa gaagctcgca caacttgaat ggaaaaatca ggcactggaa
     6481 aagaaactgg ctcaaggtgg cggaggatct gagaatctct actttcagtc cggagggtca
     6541 gggagcaaat cagtctcctc cctcgtgtcc gaatcctctc acatcgttca caaggaagat
     6601 accagtttct ggcagcattg gattaccaca aaagacgggc aggccggctc accgctggtg
     6661 tctatcattg acgggaacat tctgggaatc cactccctga cacacaccac taacggctca
     6721 aactatttcg ttgagttccc agagaagttc gtagctacat atctggacgc tgcggacggc
     6781 tggtgcaaga actggaaatt caacgcggac aagatttcat ggggtagttt cacgcttgtc
     6841 gaagacgcgc ccgaggacga ctttatggcg aaaaaaaccg tggctgctat tatggatagc
     6901 ggcggtggag gacggagcaa actgaatcca cccgatgagt ctggacccgg ttgtatgtca
     6961 tgtaaatgtg tgttgagcta gctcgaggag gcgaattcga atttaaatcg gcctcgactg
     7021 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7081 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7141 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7201 aagacaatag caggcatgct ggggacgcgg ccgcaattta acgcgaatta attctgtgga
     7261 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7321 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7381 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7441 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7501 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7561 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7621 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7681 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7741 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7801 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7861 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7921 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7981 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     8041 cggcaactgc gtgcacttcg tggccgagga gcaggactga gtcgacaatc aacctctgga
     8101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8161 tggatacgct gctttaatgc ctttgtatca tgcgttaact aaacttgttt attgcagctt
     8221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac
     8341 tcaaatgatg tcaattagtc tatcagaagc tcatctggtc tcccttccgg gggacaagac
     8401 atccctgttt aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct
     8461 ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg
     8521 gcagccaggt ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct
     8581 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     8641 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     8701 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     8761 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat
     8821 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
     8881 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
     8941 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
     9001 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
     9061 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
     9121 ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca
     9181 agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa
     9241 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc
     9301 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc
     9361 gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc
     9421 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt
     9481 gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct
     9541 ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg
     9601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
//
