LOCUS       sequence-225534-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression in E.coli and affinity purification of N-terminally 
            His tagged NQO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Tsvetkov P, Asher G, Reiss V, Shaul Y, Sachs L, Lotem J
  TITLE     Inhibition of NAD(P)H:quinone oxidoreductase 1 activity and 
            induction of p53 degradation by the natural phenolic compound 
            curcumin.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5535-40. doi: 
            10.1073/pnas.0501828102. Epub 2005 Apr 4.
  PUBMED    15809436
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0501828102"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2005-04-12- 12"; volume: "102"; issue: "15"; pages: "5535-40"
COMMENT     Sequence Label: pET28-His-TEV-NQO1
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttt tattttctag ctttgatctg
       61 gttgtcagtt gggatggact tgcccaagtg atggcccaca gaaaggccaa atttcttgtt
      121 tttctcctca tcctgtacct cttttttcat taagaatcct gcctggaagt ttaggtcaaa
      181 gaggctgctt ggagcaaaat acagtggtgt ctcatcccaa atattctcca ggcgtttctt
      241 ccatccttcc aggatttgaa ttcgggcgtc tgctggagtg tgcccaatgc tatatgtcag
      301 ttgaggttct aagacttgga agccacagaa atgcagaatg ccactctgaa ttggccagag
      361 aatgacattc atgtccccgt ggatcccttg cagagagtac atggagccac tgccaccagt
      421 ggtgatggaa agcactgcct tcttactccg gaagggtcct ttgtcataca tggcagcgta
      481 agtgtaagca aactctccta tgaacactcg ctcaaaccag cctttcagaa tggcagggac
      541 tccaaaccac tgcaggggga actggaatat cacaaggtct gcggcttcca gcttcttttg
      601 ttcagccaca atatctgggc tcagatggcc tt
//
