LOCUS       sequence-225597-        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of split luciferase fused to antiparallel 
            coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV 
            cleavage site in antiparallel harpin linker/for SPOC logic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: nLuc_AP4_TEVs_P3mS
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1660..1680
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atggagcaga agctgatcag cgaggaggac ctgggttctg gagaagatgc caaaaacatt
       61 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      121 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      181 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      241 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      301 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      361 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      421 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      481 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      541 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      601 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      661 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      721 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      781 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      841 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      901 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
      961 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1021 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1081 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1141 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1201 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1261 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1321 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1381 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1441 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1501 gaacacggta aaggcggctc tggcggcggc tccggaggct cttcacccga ggacgagctt
     1561 gctgctaatg aagaggagtt gcagcaaaat gaacaaaagt tggctcaaat taagcaaaaa
     1621 cttcaagcta tcaaatacgg tggcggctct ggcggcggcg aaaatttgta tttccagagt
     1681 ggcggctccg gaggctcttc ccccgaggac gagatccagc agctcgagga ggagatatcc
     1741 cagcttgagc agaagaactc ccagcttaag gagaagaacc agcagctgaa gtacggctaa
//
