LOCUS       sequence-225645-        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Kanamycin/neomycin resistance module for
            construction of Golden Gate cloning vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG008
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(496..515)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             507..1322
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1485..1502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1595..2183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1675..1694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3368..3472)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccacgtg tcttgtccag agctccactc tgtgcgtctc agcaattgtg tctcaaaatc
      421 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      481 acataaacag taatacaagg ggtgttatga gccatattca gcgtgaaacg agctgtagcc
      541 gtccgcgtct gaacagcaac atggatgcgg atctgtatgg ctataaatgg gcgcgtgata
      601 acgtgggtca gagcggcgcg accatttatc gtctgtatgg caaaccggat gcgccggaac
      661 tgtttctgaa acatggcaaa ggcagcgtgg cgaacgatgt gaccgatgaa atggtgcgtc
      721 tgaactggct gaccgaattt atgccgctgc cgaccattaa acattttatt cgcaccccgg
      781 atgatgcgtg gctgctgacc accgcgattc cgggcaaaac cgcgtttcag gtgctggaag
      841 aatatccgga tagcggcgaa aacattgtgg atgcgctggc cgtgtttctg cgtcgtctgc
      901 atagcattcc ggtgtgcaac tgcccgttta acagcgatcg tgtgtttcgt ctggcccagg
      961 cgcagagccg tatgaacaac ggcctggtgg atgcgagcga ttttgatgat gaacgtaacg
     1021 gctggccggt ggaacaggtg tggaaagaaa tgcataaact gctgccgttt agcccggata
     1081 gcgtggtgac ccacggcgat tttagcctgg ataacctgat tttcgatgaa ggcaaactga
     1141 ttggctgcat tgatgtgggc cgtgtgggca ttgcggatcg ttatcaggat ctggccattc
     1201 tgtggaactg cctgggcgaa tttagcccga gcctgcaaaa acgtctgttt cagaaatatg
     1261 gcattgataa tccggatatg aacaaactgc aatttcatct gatgctggat gaatttttct
     1321 aataattaat tgaactatga gacgcacgaa gtgggtacct ggagcacaag actggcctca
     1381 tgggccttca cttcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1441 catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg
     1501 ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg
     1561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2341 cttggtctga cagttattat ttacccacca ctttggtaat ttcgcctttc acgtaatgca
     2401 caaattcttc cagctgatcc gcacggctgg ccagacgatc ttcttcctgg cccagatacg
     2461 cctgacgcgc ttccagaatc accggctgat actgcgccgg cagacgttcc atggcccaat
     2521 ccgccgccac atctttcggc gcaattttgc cggtcaccgc gctataccaa atacggctca
     2581 gggtcagcac cacgttgcgt tcatcacccg cccaatccgg cgggctgttc cacagggtca
     2641 gggtttcgtt cagcgcttca aacaggtcct gttccggcac cggatcaaac agttcttccg
     2701 ctgccggacc aaccagcgcc acgctatgtt cacgcgcttt ggtcagcaga atggccagat
     2761 cgatatcaat ggtcgccggt tcaaaaatgc cggccagaat atcgttacgc tgccattcgc
     2821 caaactgcag ctcacgtttc gccggataac gccacggaat aatgtcatca tgcaccacga
     2881 tggtcacttc caccgcacgc agaatttcgc tttcacccgg gctcgcgctg gtttccagca
     2941 gatcgttaat cagcgcacga cgggtggttt catccagacg cacggtcacg gtcaccagca
     3001 gatcaatatc gctatgcggt ttcaggccgc catccaccgc gctgccatac agatgcacgg
     3061 ccagcagggt cggttccaga tgacgttcaa tcacgcccac cacttcagac agctgggtgc
     3121 tcacttccgc aatcaccgct tcacgcataa tgttcagttt ggttttcgga cggctgccct
     3181 gctgggtcac atcgttgctg ctccacagca tcaggcaacg gcccaccgca taacgcgcct
     3241 gctgtttgct cgcacgcggc atgctctgca cgccaaaaaa gcaatcatag cacgccataa
     3301 acaccgccac cgcaccatta ccgccgctac gttcggtcag ggtacggctc caattacggc
     3361 tacgcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3481 cccgaaaagt gccac
//
