LOCUS       sequence-22600-e         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Ishii T, Mombaerts P
  TITLE     Expression of nonclassical class I major histocompatibility genes 
            defines a tripartite organization of the mouse vomeronasal system.
  JOURNAL   J Neurosci. 2008 Mar 5. 28(10):2332-41.
  PUBMED    18322080
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Mar 5. 28(10):2332-41."
COMMENT     Sequence Label: pISH-Venus
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..264)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKSNFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     404..423
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gattcgattt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
       61 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
      121 gctcaggtag tggttgtcgg gcaacagcac ggggccgtct ccgatggggg tgttctgctg
      181 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttgga
      241 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta
      301 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tctatgccct tcacctcgat
      361 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      421 gtccttgaag aagatggtgc gctcctggac gtaaccttcg ggcatggcgg acttgaagaa
      481 gtcgtgctgc ttcatgtggt cagggtagcg ggcgaatcac tgcaggccgt agcccaaagt
      541 ggtcacgatg gtgggccaag gcacgggcag ctt
//
