LOCUS       sequence-226294-        5492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major 
            allele (A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, 
            Cojocaru V, Prabhakar S, Jauch R
  TITLE     DNA-mediated dimerization on a compact sequence signature controls 
            enhancer engagement and regulation by FOXA1.
  JOURNAL   Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 
            10.1093/nar/gky259.
  PUBMED    29669022
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-06-20- 20"; volume: "46"; issue: 
            "11"; pages: "5470-5486"
COMMENT     Sequence Label: rs438-major
FEATURES             Location/Qualifiers
     source          1..5492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    148..269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(159..178)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     213..232
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(517..534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(688..1276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(768..787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1476..2267,2268..2336))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2441..2489
                     /note="synthetic polyadenylation signal"
     misc_feature    2503..2594
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2543..2562
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3161..3912
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3942..3951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
       61 agattctcat taaggccaag aagggcggca agatcgccgt gtaataattc tagagtcggg
      121 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
      181 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      241 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      301 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      361 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
      421 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
      481 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1441 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
     1501 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
     1561 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
     1621 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
     1681 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
     1741 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
     1801 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
     1861 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
     1921 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
     1981 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
     2041 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
     2101 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
     2161 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
     2221 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
     2281 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
     2341 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa
     2401 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc
     2461 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
     2521 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
     2581 cagaacattt ctctggccta actggccggt accccgtgtt gcatctgatc ctctggcccc
     2641 tcaagacaaa gtcaatctag gtttagaaca gttagagctc ttatagctgt atccatgtgt
     2701 gccgatatta aatgtcgacc aaagacagag cacgttgtgc taattgtaat acatgcacct
     2761 ataagcatgc aaattggctt tggtcagaac tgaaattccc agcagccccc aggcctcctg
     2821 taaggaaaca tgtattatca gattttcttc aaaggtaaag aagagtcagt tgttaggtgc
     2881 ctgagttaat tacataaaaa taccacctga ggcttaatca aatattttct gattagcagt
     2941 cagtacaaca gctctaatct aataaactga caatcagcgg taatgggagc tgctagacag
     3001 gagggaagac ccttctttgg tgttagggcc actctgtgac cagtcattgc tcaggctttc
     3061 tttgtctgga tgacctggtt tccatagtgc tgggagaaag ctcagcataa gtagaaatgc
     3121 ctttgaaaaa gatatgaatt gttggggtgg gtgatatcta aatgagtctt cggacctcgc
     3181 gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa
     3241 acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca cgcagaagag
     3301 cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt ggtcggcggg
     3361 tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg
     3421 tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct gggtcctagg
     3481 ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg cggtgatatt
     3541 accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg tccgttgggc
     3601 gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg cgagggactg
     3661 caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct cgcgtttgct
     3721 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     3781 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     3841 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     3901 cgacccgctt aaaagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
     3961 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
     4021 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
     4081 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
     4141 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
     4201 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
     4261 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
     4321 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
     4381 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
     4441 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     4501 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     4561 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     4621 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     4681 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     4741 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     4801 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     4861 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     4921 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     4981 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     5041 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     5101 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     5161 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     5221 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     5281 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     5341 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     5401 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     5461 aggttacaac cgccaagaag ctgcgcggtg gt
//
