LOCUS       sequence-226459-       10024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of mutant human Tumor Protein p53 (TP53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX313-TP53-P278A
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1539..1749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1561..1757
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1608..1743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1779..2798
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    2869..2986
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3124..4302
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3355..4293
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4250..4270
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4322..4346
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4346..5524
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACAGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(5526..5550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5558..5599
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5650..6238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5703..5723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6121..6132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6310..6543
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6621..6742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6658..6677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6712..6731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6782..6917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6937..6956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6938..6956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6966..6983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6966..6982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6975..6997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7124..7579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7211..7230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7421..7442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7605..7709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7710..7778,7779..8570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7928..7947)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8741..9329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9230..9249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9483..9500
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9617..9638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9653..9658,9659..9676,9677..9683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9691..9707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9696..9718
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9715..9731
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9715..9731
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9750..9770
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9752..9770
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9798..10024
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccat gaaaaagcct gaactcaccg
     1801 ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     1861 tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc
     1921 tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg
     1981 catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga
     2041 cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac
     2101 tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta
     2161 gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc
     2221 gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     2281 acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact
     2341 gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca
     2401 atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg
     2461 aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct
     2521 acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc
     2581 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     2641 gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac
     2701 aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata
     2761 gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa tgaggcgcgc
     2821 cgttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2881 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2941 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     3001 ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag
     3061 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     3121 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     3181 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     3241 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     3301 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     3361 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     3421 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     3481 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     3541 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     3601 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     3661 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     3721 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     3781 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     3841 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     3901 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     3961 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     4021 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     4081 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     4141 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     4201 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     4261 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggctagca tcgattgatc
     4321 aacaagtttg tacaaaaaag ttggcatgga ggagccgcag tcagatccta gcgtcgagcc
     4381 ccctctgagt caggaaacat tttcagacct atggaaacta cttcctgaaa acaacgttct
     4441 gtcccccttg ccgtcccaag caatggatga tttgatgctg tccccggacg atattgaaca
     4501 atggttcact gaagacccag gtccagatga agctcccaga atgccagagg ctgctccccg
     4561 cgtggcccct gcaccagcag ctcctacacc ggcggcccct gcaccagccc cctcctggcc
     4621 cctgtcatct tctgtccctt cccagaaaac ctaccagggc agctacggtt tccgtctggg
     4681 cttcttgcat tctgggacag ccaagtctgt gacttgcacg tactcccctg ccctcaacaa
     4741 gatgttttgc caactggcca agacctgccc tgtgcagctg tgggttgatt ccacaccccc
     4801 gcccggcacc cgcgtccgcg ccatggccat ctacaagcag tcacagcaca tgacggaggt
     4861 tgtgaggcgc tgcccccacc atgagcgctg ctcagatagc gatggtctgg cccctcctca
     4921 gcatcttatc cgagtggaag gaaatttgcg tgtggagtat ttggatgaca gaaacacttt
     4981 tcgacatagt gtggtggtgc cctatgagcc gcctgaggtt ggctctgact gtaccaccat
     5041 ccactacaac tacatgtgta acagttcctg catgggcggc atgaaccgga ggcccatcct
     5101 caccatcatc acactggaag actccagtgg taatctactg ggacggaaca gctttgaggt
     5161 gcgtgtttgt gcctgtgctg ggagagaccg gcgcacagag gaagagaatc tccgcaagaa
     5221 aggggagcct caccacgagc tgcccccagg gagcactaag cgagcactgc ccaacaacac
     5281 cagctcctct ccccagccaa agaagaaacc actggatgga gaatatttca cccttcagat
     5341 ccgtgggcgt gagcgcttcg agatgttccg agagctgaat gaggccttgg aactcaagga
     5401 tgcccaggct gggaaggagc caggggggag cagggctcac tccagccacc tgaagtccaa
     5461 aaagggtcag tctacctccc gccataaaaa actcatgttc aagacagaag ggcctgactc
     5521 agactgccca actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct
     5581 cctcggtctc gattctacgt agtaatgaac tagtaccggt taagtcgaca atcaacgcgt
     5641 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5701 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5761 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5821 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5881 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5941 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6001 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6061 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6121 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6181 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6241 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6301 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6541 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6601 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6721 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6781 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6841 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6901 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6961 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7021 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7081 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7141 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7201 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7261 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7321 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7381 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7441 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7501 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7561 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7621 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7681 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7741 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7801 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7861 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7921 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7981 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8041 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8101 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8161 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8221 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8281 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8341 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8401 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8461 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8521 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8581 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8641 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8701 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8761 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8821 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8881 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8941 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9001 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9061 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9121 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9181 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9241 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9301 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9361 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9421 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9481 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9541 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9601 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9661 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9721 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9781 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9841 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9901 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9961 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
    10021 aaac
//
