LOCUS       sequence-226475-       15458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-144
FEATURES             Location/Qualifiers
     source          1..15458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             744..1454
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1471..1565
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1609..2268
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1675..1694)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2333..2419
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2848..3726)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3753..4037
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3960..3979
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(10728..10745)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10898..10984
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11076..11103
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        11122..11213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11214..11282,11283..12074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11432..11451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(12203..12222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            12245..12354
                     /note="incP origin of transfer"
     primer_bind     complement(12392..12411)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      13094..13316
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             13364..14314
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  14747..14912
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        join(15090..15095,15096..15112,15113..15118)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(15307..15458,1..382)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
       61 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
      121 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
      181 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
      241 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
      301 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
      361 cgacccaagt accgccacct aaagctcgca cgctgccgca agcactcagg gcgcaagggc
      421 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
      481 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
      541 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
      601 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
      661 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
      721 ggatactagt ggaggaagaa aaaatggtga gcaagggcga ggagaataac atggccatca
      781 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
      841 agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct aagctgaagg
      901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat ttcacctacg
      961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag ctgtccttcc
     1021 ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg gtgaccgtga
     1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1141 acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctcctccg
     1201 agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg ctgaagctga
     1261 aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag aagcccgtgc
     1321 agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac aacgaggact
     1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1441 agctgtacaa gtaataatta gctgagcttg gactcctgtt gatagatcca gtaatgacct
     1501 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     1561 agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1621 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1681 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1741 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1801 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
     1861 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1921 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1981 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2041 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2101 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2161 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     2221 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     2281 agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa
     2341 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2401 tctcctgagt aggacaaatc cgccgctagg agcttgcggc cgccaggggt caatagcggc
     2461 cgccactcga gcacctagga ggcgcgccac ggccgcgtcg accccacgcc cctctttaat
     2521 acgacgggca atttgcactt cagaaaatga agagtttgct ttagccataa caaaagtcca
     2581 gtatgctttt tcacagcata actggactga tttcagttta caactattct gtctagttta
     2641 agactttatt gtcatagttt agatctattt tgttcagttt aagactttat tgtccgccca
     2701 caggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tcatcgatgc
     2761 ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa
     2821 gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt
     2881 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
     2941 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
     3001 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
     3061 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
     3121 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
     3181 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
     3241 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
     3301 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
     3361 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
     3421 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     3481 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     3541 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
     3601 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     3661 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
     3721 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     3781 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     3841 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     3901 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     3961 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
     4021 ctctactgtt tctccatacc cgtttttttg ggctagcgaa ttcgagctcg gtacccaagt
     4081 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
     4141 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
     4201 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattccatgg ctaacagtac
     4261 aagaagttcc ttcttcaggt cgttcccacc gtatttattc tcataagacg ggacgagtcc
     4321 atcatttgct atctgactta gagcttgctg tttttctcag tcttgagtgg gagagcagcg
     4381 tgctagatat acgcgagcag ttccccttat tacctagtga taccaggcag attgcaatag
     4441 atagtggtat taagcatcct gttattcgtg gtgtagatca ggttatgtct actgattttt
     4501 tagtggactg caaagatggt ccttttgagc agtttgctat tcaagtcaaa cctgcagcag
     4561 ccttacaaga cgagcgtacc ttagaaaaac tagaactaga gcgtcgctat tggcagcaaa
     4621 agcaaattcc ttggttcatt tttactgata aagaaataaa tcccgtagta aaagaaaata
     4681 ttgaatggct ttattcagtg aaaacagaag aagtttctgc ggagctttta gcacaactat
     4741 ccccattggc ccatatcctg caagaaaaag gagatgaaaa cattatcaat gtctgtaagc
     4801 aggttgatat tgcttatgat ttggagttag gcaaaacatt gagtgagata cgagccttaa
     4861 ccgcaaatgg ttttattaag ttcaatattt ataagtcttt cagggcaaat aagtgtgcag
     4921 atctctgtat tagccaagta gtgaatatgg aggagttgcg ctatgtggca aattaatgag
     4981 gttgtgctat ttgataatga tccgtatcgc attttggcta tagaggatgg ccaagttgtc
     5041 tggatgcaaa taagcgctga taaaggagtt ccacaagcta gggctgagtt gttgctaatg
     5101 cagtatttag atgaaggccg cttagttaga actgatgacc cttatgtaca tcttgattta
     5161 gaagagccgt ctgtagattc tgtcagcttc cagaagcgcg aggaggatta tcgaaaaatt
     5221 cttcctatta ttaatagtaa ggatcgtttc gaccctaaag tcagaagcga actcgttgag
     5281 catgtggtcc aagaacataa ggttactaag gctacagttt ataagttgtt acgccgttac
     5341 tggcagcgtg gtcaaacgcc taatgcatta attcctgact acaaaaacag cggtgcacca
     5401 ggggaaagac gttcagcgac aggaacagca aagattggcc gagccagaga atatggtaag
     5461 ggtgaaggaa ccaaggtaac gcccgagatt gaacgccttt ttaggttgac catagaaaag
     5521 cacctgttaa atcaaaaagg tacaaagacc accgttgcct atagacgatt tgtggacttg
     5581 tttgctcagt attttcctcg cattccccaa gaggattacc caacactacg tcagtttcgt
     5641 tatttttatg atcgagaata ccctaaagct cagcgcttaa agtctagagt taaagcaggg
     5701 gtatataaaa aagacgtacg acccttaagt agtacagcca cttctcaggc gttaggccct
     5761 gggagtcgtt atgagattga tgccacgatt gctgatattt atttagtgga tcatcatgat
     5821 cgccaaaaaa tcataggaag accaacgctt tacattgtga ttgatgtgtt tagtcggatg
     5881 atcacgggct tttatatcgg ctttgaaaat ccgtcttatg tggtggcgat gcaggctttt
     5941 gtaaatgctt gctctgacaa aacggccatt tgtgcccagc atgatattga gattagtagc
     6001 tcagactggc cgtgtgtagg tttgccagat gtgttgctag cggaccgtgg cgaattaatg
     6061 agtcatcagg tcgaagcctt agtttctagt tttaatgtgc gagtggaaag tgctccacct
     6121 agacgtggcg atgctaaagg catagtggaa agcactttta gaacactaca agccgagttt
     6181 aagtcctttg cacctggcat tgtagagggc agtcggatca aaagccatgg tgaaacagac
     6241 tataggttag atgcatctct gtcggtattt gagttcacac aaattatttt gcgtacgatc
     6301 ttattcagaa ataaccatct ggtgatggat aaatacgatc gagatgctga ttttcctaca
     6361 gatttaccgt ctattcctgt ccagctatgg caatggggta tgcagcatcg tacaggtagt
     6421 ttaagggctg tggagcaaga gcagttgcga gtagcgttac tgcctcgccg aaaggtctct
     6481 atttcttcat ttggcgttaa tttgtggggt ttgtattact cggggtcaga gattctgcgt
     6541 gagggttggt tgcagcggag cactgatata gctagacctc aacatttaga agcggcttat
     6601 gacccagtgc tggttgatac gatttatttg tttccgcaag ttggcagccg tgtattttgg
     6661 cgctgtaatc tgacggaacg tagtcggcag tttaaaggtc tctcattttg ggaggtttgg
     6721 gatatacaag cacaagaaaa acacaataaa gccaatgcga agcaggatga gttaactaaa
     6781 cgcagggagc ttgaggcgtt tattcagcaa accattcaga aagcgaataa gttaacgccc
     6841 agtactactg agcccaaatc aacacgcatt aagcagatta aaactaataa aaaagaagcc
     6901 gtgacctcgg agcgtaaaaa acgtgcggag catttgaagc caagctcttc aggtgatgag
     6961 gctaaagtta ttcctttcaa cgcagtggaa gcggatgatc aagaagatta cagcctaccc
     7021 acatacgtgc ctgaattatt tcaggatcca ccagaaaagg atgagtcatg agtgctaccc
     7081 ggattcaagc agtttatcgt gatacggggg tagaggctta tcgtgataat ccttttatcg
     7141 aggccttacc accattacaa gagtcagtga atagtgctgc atcactgaaa tcctctttac
     7201 agcttacttc ctctgacttg caaaagtccc gtgttatcag agctcatacc atttgtcgta
     7261 ttccagatga ctattttcag ccattaggta cgcatttgct actaagtgag cgtatttcgg
     7321 tcatgattcg aggtggctac gtaggcagaa atcctaaaac aggagattta caaaagcatt
     7381 tacaaaatgg ttatgagcgt gttcaaacgg gagagttgga gacatttcgc tttgaggagg
     7441 cacgatctac ggcacaaagc ttattgttaa ttggttgttc tggtagtggg aagacgacct
     7501 ctcttcatcg tattctagcc acgtatcctc aggtgattta ccatcgtgaa ctcaatgtag
     7561 agcaggtggt gtatttgaaa atagactgct cgcataatgg ttcgctaaaa gaaatctgct
     7621 tgaatttttt cagagcgttg gatcgagcct tgggctcgaa ctatgagcgt cgttatggct
     7681 taaaacgtca tggtatagaa accatgttgg ctttgatgtc gcaaatagcc aatgcacatg
     7741 ctttagggtt gttggttatt gatgaaattc agcatttaag ccgctctcgt tcgggtggat
     7801 ctcaagagat gctgaacttt tttgtgacga tggtgaatat tattggcgta ccagtgatgt
     7861 tgattggtac ccctaaagca cgagagattt ttgaggctga tttgcggtct gcacgtagag
     7921 gggcagggtt tggagctata ttctgggatc ctatacaaca aacgcaacgt ggaaagccca
     7981 atcaagagtg gatcgctttt acggataatc tttggcaatt acagctttta caacgcaaag
     8041 atgcgctgtt atcggatgag gtccgtgatg tgtggtatga gctaagccaa ggagtgatgg
     8101 acattgtagt aaaacttttt gtactcgctc agctccgtgc gctagcttta ggcaatgagc
     8161 gtattaccgc tggtttattg cggcaagtgt atcaagatga gttaaagcct gtgcacccca
     8221 tgctagaggc attacgctcg ggtatcccag aacgcattgc tcgttattct gatctagtcg
     8281 ttcccgagat tgataaacgg ttaatccaac ttcagctaga tatcgcagcg atacaagaac
     8341 aaacaccaga agaaaaagcc cttcaagagt tagataccga agatcagcgt catttatatc
     8401 tgatgctgaa agaggattac gattcaagcc tgttaattcc cactattaaa aaagcgttta
     8461 gccagaatcc aacgatgaca agacaaaagt tactgcctct tgttttgcag tggttgatgg
     8521 aaggcgaaac ggtagtgtca gaactagaaa agccctccaa gagtaaaaag gtttcggcta
     8581 taaaggtagt caagcccagc gactgggata gcttgcctga tacggattta cgttatatct
     8641 attcacaacg ccaacctgaa aaaaccatgc atgaacggtt aaaagggaaa ggggtaatag
     8701 tggatatggc gagcttattt aaacaagcag gttagccatg agaaactttc ctgttccgta
     8761 ctcgaatgag ctgatttata gcactattgc acgggcaggc gtttatcaag ggattgttag
     8821 tcctaagcag ctgttggatg aggtgtatgg caaccgcaag gtggtcgcta ccttaggtct
     8881 gccctcgcat ttaggtgtga tagcaagaca tctacatcaa acaggacgtt acgctgttca
     8941 gcagcttatt tatgagcata ccttattccc tttatatgct ccgtttgtag gcaaggagcg
     9001 ccgagacgaa gctattcggt taatggagta ccaagcgcaa ggtgcggtgc atttaatgct
     9061 aggagtcgct gcttctagag ttaagagcga taaccgcttt agatactgcc ctgattgcgt
     9121 tgctcttcag ctaaataggt atggggaagc cttttggcaa cgagattggt atttgcccgc
     9181 tttgccatat tgtccaaaac acggtgcttt agtcttcttt gatagagctg tagatgatca
     9241 ccgacatcaa ttttgggctt tgggtcatac tgagctgctt tcagactacc ccaaagactc
     9301 cctatctcaa ttaacagcac tagctgctta tatagcccct ctgttagatg ctccacgagc
     9361 gcaagagctt tccccaagcc ttgagcagtg gacgctgttt tatcagcgct tagcgcagga
     9421 tctagggcta accaaaagca agcacattcg tcatgacttg gtggcggaga gagtgaggca
     9481 gacttttagt gatgaggcac tagagaaact ggatttaaag ttggcagaga acaaggacac
     9541 gtgttggctg aaaagtatat tccgtaagca tagaaaagcc tttagttatt tacagcatag
     9601 tattgtgtgg caagccttat tgccaaaact aacggttata gaagcgctac agcaggcaag
     9661 tgctcttact gagcactcta taacgacaag acctgttagc cagtctgtgc aacctaactc
     9721 tgaagattta tctgttaagc ataaagactg gcagcaacta gtgcataaat accaaggaat
     9781 taaggcggca agacagtctt tagagggtgg ggtgctatac gcttggcttt accgacatga
     9841 cagggattgg ctagttcact ggaatcaaca gcatcaacaa gagcgtctgg cacccgcccc
     9901 tagagttgat tggaaccaaa gagatcgaat tgctgtacga caactattaa gaatcataaa
     9961 gcgtctagat agtagccttg atcacccaag agcgacatcg agctggctgt taaagcaaac
    10021 tcctaacgga acctctcttg caaaaaatct acagaaactg cctttggtag cgctttgctt
    10081 aaagcgttac tcagagagtg tggaagatta tcaaattaga cggattagcc aagcttttat
    10141 taagcttaaa caggaagatg ttgagcttag gcgctggcga ttattaagaa gtgcaacgtt
    10201 atctaaagag cggataactg aggaagcaca aagattcttg gaaatggttt atggggaaga
    10261 gtgagtggtt aggctagcta catttaatga caatgtgcag gttgtacata ttggtcattt
    10321 attccgtaac tcgggtcata aggagtggcg tatttttgtt tggtttaatc caatgcaaga
    10381 acggaaatgg actcgattta ctcatttgcc tttattaagt cgagctaagg tggttaacag
    10441 tacaacaaag caaataaata aggcggatcg tgtgattgag tttgaagcat cggatcttca
    10501 acgagccaaa ataatcgatt ttcctaatct ctcgtccttt gcttccgtac gcaacaagga
    10561 tggagcgcag agttcattta tttacgaagc tgaaacacca tatagcaaga ctcgttatca
    10621 catcccacag ttagagctag ctcggtcatt atttttaggg gatcctctag agtcgacctg
    10681 caggcatgca agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt
    10741 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
    10801 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
    10861 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
    10921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
    10981 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
    11041 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
    11101 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
    11161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    11221 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11281 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    11341 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    11401 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
    11461 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11521 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11581 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11641 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11701 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
    11761 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11821 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11881 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11941 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    12001 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12061 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacgcg
    12121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    12181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    12241 gccgccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
    12301 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggcggc
    12361 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
    12421 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
    12481 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
    12541 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
    12601 gagcgtcgat ccccgacagt aagacgggta agcctgttga tgataccgct gccttactgg
    12661 gtgcattagc cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa
    12721 tcagagggca ggaactgctg aacagcaaaa agtcagatag caccacatag cagacccgcc
    12781 ataaaacgcc ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag tttccttgca
    12841 tgaatccata aaaggcgcct gtagtgccat ttacccccat tcactgccag agccgtgagc
    12901 gcagcgaact gaatgtcacg aaaaagacag cgactcaggt gcctgatggt cggagacaaa
    12961 aggaatattc agcgatttgc ccgagcttgc gagggtgcta cttaagcctt tagggtttta
    13021 aggtctgttt tgtagaggag caaacagcgt ttgcgacatc cttttgtaat actgcggaac
    13081 tgactaaagt agtgagttat acacagggct gggatctatt ctttttatct ttttttattc
    13141 tttctttatt ctataaatta taaccacttg aatataaaca aaaaaaacac acaaaggtct
    13201 agcggaattt acagagggtc tagcagaatt tacaagtttt ccagcaaagg tctagcagaa
    13261 tttacagata cccacaactc aaaggaaaag gactagtaat tatcattgac tagcccatct
    13321 caattggtat agtgattaaa atcacctaga ccaattgaga tgtatgtctg aattagttgt
    13381 tttcaaagca aatgaactag cgattagtcg ctatgactta acggagcatg aaaccaagct
    13441 aattttatgc tgtgtggcac tactcaaccc cacgattgaa aaccctacaa ggaaagaacg
    13501 gacggtatcg ttcacttata accaatacgt tcagatgatg aacatcagta gggaaaatgc
    13561 ttatggtgta ttagctaaag caaccagaga gctgatgacg agaactgtgg aaatcaggaa
    13621 tcctttggtt aaaggctttg agattttcca gtggacaaac tatgccaagt tctcaagcga
    13681 aaaattagaa ttagttttta gtgaagagat attgccttat cttttccagt taaaaaaatt
    13741 cataaaatat aatctggaac atgttaagtc ttttgaaaac aaatactcta tgaggattta
    13801 tgagtggtta ttaaaagaac taacacaaaa gaaaactcac aaggcaaata tagagattag
    13861 ccttgatgaa tttaagttca tgttaatgct tgaaaataac taccatgagt ttaaaaggct
    13921 taaccaatgg gttttgaaac caataagtaa agatttaaac acttacagca atatgaaatt
    13981 ggtggttgat aagcgaggcc gcccgactga tacgttgatt ttccaagttg aactagatag
    14041 acaaatggat ctcgtaaccg aacttgagaa caaccagata aaaatgaatg gtgacaaaat
    14101 accaacaacc attacatcag attcctacct acataacgga ctaagaaaaa cactacacga
    14161 tgctttaact gcaaaaattc agctcaccag ttttgaggca aaatttttga gtgacatgca
    14221 aagtaagtat gatctcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact
    14281 agagaacata ctggctaaat acggaaggat ctgaggttct tatggctctt gtatctatca
    14341 gtgaagcatc aagactaaca aacaaaagta gaacaactgt tcaccgttac atatcaaagg
    14401 gaaaactgtc catatgcaca gatgaaaacg gtgtaaaaaa gatagataca tcagagcttt
    14461 tacgagtttt tggtgcattt aaagctgttc accatgaaca gatcgacaat gtaacagatg
    14521 aacagcatgt aacacctaat agaacaggtg aaaccagtaa aacaaagcaa ctagaacatg
    14581 aaattgaaca cctgagacaa cttgttacag ctcaacagtc acacatagac agcctgaaac
    14641 aggcgatgct gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg acgcctcccg
    14701 tggggaaaaa atcatggcaa ttctggaaga aatagcgctt tcagcctgtg ggcggacaaa
    14761 atagttggga actgggaggg gtggaaatgg agtttttaag gattatttag ggaagagtga
    14821 caaaatagat gggaactggg tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa
    14881 atagtttgga actagatttc acttatctgg ttggtcgaca ctagtattac cctgttatcc
    14941 ctagatttaa atgatatcgg atcctagtaa gccacgtttt aattaatcag atcccataaa
    15001 ctgccaggaa ttggggatcg gattcgagct ttagtaccta gatttagatg tctaaaaagc
    15061 ggggtaccga ggacgcgtca attctcgaat tgacataagc ctgttcggtt cgtaaactgt
    15121 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    15181 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
    15241 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
    15301 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
    15361 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
    15421 tccatgcggg ctgctcttga tcttttcggt cgtgagtt
//
