LOCUS       sequence-226478-        4330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses araC constitutively and mScarletI (RFP) under a pBAD 
            promoter; alternate name pSB1A2-AD011.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Dupin A, Simmel FC
  TITLE     Signalling and differentiation in emulsion-based 
            multi-compartmentalized in vitro gene circuits.
  JOURNAL   Nat Chem. 2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9.
  PUBMED    30478365
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9."
COMMENT     Sequence Label: pSB1A3-AD011
FEATURES             Location/Qualifiers
     source          1..4330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     413..432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(651..755)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     823..841
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(845..888)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    891..912
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(998..1876)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1903..2187
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2110..2129
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             2363..2374
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    3164..3184
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(3190..3207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3361..3949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3441..3460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4120..4330,1..581,582..650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
       61 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      121 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      181 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      241 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      301 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      361 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      421 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      481 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      541 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      601 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      661 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      721 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      781 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      841 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
      901 ccgcttctag aggagagtta ggcataaggc tgcatgctac cctttggtcg aaaaaaaaag
      961 cccgcactgt caggtgcggg cttttttctg tgtttcctta tgacaacttg acggctacat
     1021 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     1081 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     1141 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     1201 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     1261 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     1321 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     1381 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     1441 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     1501 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     1561 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     1621 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     1681 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     1741 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     1801 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     1861 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     1921 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     1981 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     2041 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     2101 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     2161 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcgaa ttcgagctcg
     2221 gtacccgggg atcctctaga aggagatata accagcgctc aacgggtgtg cttcccgttc
     2281 tgatgagtcc gtgaggacga aagcgcctct acaaataatt ttgtttaagg agctatggac
     2341 tatgtttgaa aggctgctcg agaaagagga gaaaatggtg tcaaagggag aggcggttat
     2401 caaggaattt atgcgcttta aagtccacat ggagggcagc atgaacgggc acgagtttga
     2461 aattgagggg gagggggagg gccgtcctta tgaaggtact cagactgcta aactgaaggt
     2521 gacaaaaggt ggccccttgc ctttctcgtg ggacatcctg tcgccacaat tcatgtacgg
     2581 gagccgcgcc tttatcaaac atcccgcaga tattcctgat tactataaac aatctttccc
     2641 ggaaggtttc aaatgggaac gcgtcatgaa ttttgaggac gggggcgctg tcacagttac
     2701 tcaggacacc tccttggaag acggcacatt gatttacaag gttaagttgc gcggcacaaa
     2761 cttcccccct gacgggccag taatgcaaaa gaaaactatg ggttgggagg cgtctacaga
     2821 acgtttatac cccgaagacg gggtgctgaa aggtgacatt aagatggccc tgcgcctgaa
     2881 ggacggcggt cgctatcttg ccgactttaa aactacttat aaggctaaaa aaccagtcca
     2941 gatgccaggc gcctataatg ttgaccgcaa gttagacatc acctcacata atgaagacta
     3001 taccgttgta gaacaatacg agcgcagcga gggtcgtcac agtaccgggg ggatggatga
     3061 attatacaaa taaaagcttt agaacgcatg agaaagcccc cggaagatca ccttccgggg
     3121 gcttttttat tgcgccttgt cacacctaca ctgctcgaag tactactagt agcggccgct
     3181 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3361 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4021 cattagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4321 gcgcagaagt
//
