LOCUS       sequence-226506-       15458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-140
FEATURES             Location/Qualifiers
     source          1..15458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(351..356,357..373,374..379)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             568..1101
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             1463..2173
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2190..2284
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2328..2987
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2394..2413)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3052..3138
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  3249..3414
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(3847..4797)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4845..5067
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     5750..5769
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            5807..5916
                     /note="incP origin of transfer"
     primer_bind     5939..5958
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(6087..6878,6879..6947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6710..6729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6948..7039)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(7058..7085)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7177..7263)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     7416..7433
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(14124..14408)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(14182..14201)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             14435..15313
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggg gtcgacgcgg ccgtggcgcg cctcctaggt gctcgagtgg
      241 cggccgctat tsasccataa actgccagga attggggatc ggattcgagc tttagtacct
      301 agatttagat gtctaaaaag cggggtaccg aggacgcgtc aattctcgaa ttgacataag
      361 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      421 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      481 gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      541 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
      601 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg
      661 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
      721 ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc
      781 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
      841 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc
      901 gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
      961 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
     1021 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
     1081 gacccaagta ccgccaccta aagctcgcac gctgccgcaa gcactcaggg cgcaagggct
     1141 gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa acggtgctga ccccggatga
     1201 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag
     1261 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac
     1321 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
     1381 tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag
     1441 gatactagtg gaggaagaaa aaatggtgtc taagggcgaa gagctgatta aggagaacat
     1501 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     1561 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     1621 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     1681 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     1741 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     1801 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     1861 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
     1921 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     1981 tctgatcgca aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     2041 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     2101 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     2161 gcacaagctt aattaattag ctgagcttgg actcctgttg atagatccag taatgacctc
     2221 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     2281 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     2341 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     2401 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     2461 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     2521 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     2581 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     2641 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     2701 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     2761 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     2821 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     2881 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     2941 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     3001 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     3061 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3121 ctcctgagta ggacaaatcc gccgctagga gcttgcggcc gccaggggat ctgattaatt
     3181 aaaacgtggc ttactaggat ccgatatcat ttaaatctag ggataacagg gtaatactag
     3241 tgtcgaccaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     3301 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     3361 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacaggctga
     3421 aagcgctatt tcttccagaa ttgccatgat tttttcccca cgggaggcgt cactggctcc
     3481 cgtgttgtcg gcagctttga ttcgataagc agcatcgcct gtttcaggct gtctatgtgt
     3541 gactgttgag ctgtaacaag ttgtctcagg tgttcaattt catgttctag ttgctttgtt
     3601 ttactggttt cacctgttct attaggtgtt acatgctgtt catctgttac attgtcgatc
     3661 tgttcatggt gaacagcttt aaatgcacca aaaactcgta aaagctctga tgtatctatc
     3721 ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat gtaacggtga
     3781 acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata
     3841 agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     3901 cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt
     3961 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     4021 tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     4081 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     4141 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     4201 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     4261 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     4321 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt
     4381 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     4441 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     4501 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     4561 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     4621 catcatctga acgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     4681 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     4741 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     4801 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     4861 attactagtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     4921 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     4981 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     5041 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     5101 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     5161 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     5221 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     5281 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
     5341 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
     5401 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     5461 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     5521 tcaacaggct tacccgtctt actgtcgggg atcgacgctc tcccttatgc gactcctgca
     5581 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     5641 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     5701 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     5761 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccgccgggc aggataggtg
     5821 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     5881 tgctcaacgg gaatcctgct ctgcgaggct ggccggcggc gaacgtggcg agaaaggaag
     5941 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6001 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaatc aatctaaagt
     6061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     6421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     6721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7021 atttagaaaa ataaacaaaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     7081 cttctgctta atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     7141 ccgttgcttc gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     7201 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     7261 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
     7321 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
     7381 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
     7441 tcttctctca tccgccaaaa cagccaagct tgcatgcctg caggtcgact ctagaggatc
     7501 ccctaaaaat aatgaccgag ctagctctaa ctgtgggatg tgataacgag tcttgctata
     7561 tggtgtttca gcttcgtaaa taaatgaact ctgcgctcca tccttgttgc gtacggaagc
     7621 aaaggacgag agattaggaa aatcgattat tttggctcgt tgaagatccg atgcttcaaa
     7681 ctcaatcaca cgatccgcct tatttatttg ctttgttgta ctgttaacca ccttagctcg
     7741 acttaataaa ggcaaatgag taaatcgagt ccatttccgt tcttgcattg gattaaacca
     7801 aacaaaaata cgccactcct tatgacccga gttacggaat aaatgaccaa tatgtacaac
     7861 ctgcacattg tcattaaatg tagctagcct aaccactcac tcttccccat aaaccatttc
     7921 caagaatctt tgtgcttcct cagttatccg ctctttagat aacgttgcac ttcttaataa
     7981 tcgccagcgc ctaagctcaa catcttcctg tttaagctta ataaaagctt ggctaatccg
     8041 tctaatttga taatcttcca cactctctga gtaacgcttt aagcaaagcg ctaccaaagg
     8101 cagtttctgt agattttttg caagagaggt tccgttagga gtttgcttta acagccagct
     8161 cgatgtcgct cttgggtgat caaggctact atctagacgc tttatgattc ttaatagttg
     8221 tcgtacagca attcgatctc tttggttcca atcaactcta ggggcgggtg ccagacgctc
     8281 ttgttgatgc tgttgattcc agtgaactag ccaatccctg tcatgtcggt aaagccaagc
     8341 gtatagcacc ccaccctcta aagactgtct tgccgcctta attccttggt atttatgcac
     8401 tagttgctgc cagtctttat gcttaacaga taaatcttca gagttaggtt gcacagactg
     8461 gctaacaggt cttgtcgtta tagagtgctc agtaagagca cttgcctgct gtagcgcttc
     8521 tataaccgtt agttttggca ataaggcttg ccacacaata ctatgctgta aataactaaa
     8581 ggcttttcta tgcttacgga atatactttt cagccaacac gtgtccttgt tctctgccaa
     8641 ctttaaatcc agtttctcta gtgcctcatc actaaaagtc tgcctcactc tctccgccac
     8701 caagtcatga cgaatgtgct tgcttttggt tagccctaga tcctgcgcta agcgctgata
     8761 aaacagcgtc cactgctcaa ggcttgggga aagctcttgc gctcgtggag catctaacag
     8821 aggggctata taagcagcta gtgctgttaa ttgagatagg gagtctttgg ggtagtctga
     8881 aagcagctca gtatgaccca aagcccaaaa ttgatgtcgg tgatcatcta cagctctatc
     8941 aaagaagact aaagcaccgt gttttggaca atatggcaaa gcgggcaaat accaatctcg
     9001 ttgccaaaag gcttccccat acctatttag ctgaagagca acgcaatcag ggcagtatct
     9061 aaagcggtta tcgctcttaa ctctagaagc agcgactcct agcattaaat gcaccgcacc
     9121 ttgcgcttgg tactccatta accgaatagc ttcgtctcgg cgctccttgc ctacaaacgg
     9181 agcatataaa gggaataagg tatgctcata aataagctgc tgaacagcgt aacgtcctgt
     9241 ttgatgtaga tgtcttgcta tcacacctaa atgcgagggc agacctaagg tagcgaccac
     9301 cttgcggttg ccatacacct catccaacag ctgcttagga ctaacaatcc cttgataaac
     9361 gcctgcccgt gcaatagtgc tataaatcag ctcattcgag tacggaacag gaaagtttct
     9421 catggctaac ctgcttgttt aaataagctc gccatatcca ctattacccc tttccctttt
     9481 aaccgttcat gcatggtttt ttcaggttgg cgttgtgaat agatataacg taaatccgta
     9541 tcaggcaagc tatcccagtc gctgggcttg actaccttta tagccgaaac ctttttactc
     9601 ttggagggct tttctagttc tgacactacc gtttcgcctt ccatcaacca ctgcaaaaca
     9661 agaggcagta acttttgtct tgtcatcgtt ggattctggc taaacgcttt tttaatagtg
     9721 ggaattaaca ggcttgaatc gtaatcctct ttcagcatca gatataaatg acgctgatct
     9781 tcggtatcta actcttgaag ggctttttct tctggtgttt gttcttgtat cgctgcgata
     9841 tctagctgaa gttggattaa ccgtttatca atctcgggaa cgactagatc agaataacga
     9901 gcaatgcgtt ctgggatacc cgagcgtaat gcctctagca tggggtgcac aggctttaac
     9961 tcatcttgat acacttgccg caataaacca gcggtaatac gctcattgcc taaagctagc
    10021 gcacggagct gagcgagtac aaaaagtttt actacaatgt ccatcactcc ttggcttagc
    10081 tcataccaca catcacggac ctcatccgat aacagcgcat ctttgcgttg taaaagctgt
    10141 aattgccaaa gattatccgt aaaagcgatc cactcttgat tgggctttcc acgttgcgtt
    10201 tgttgtatag gatcccagaa tatagctcca aaccctgccc ctctacgtgc agaccgcaaa
    10261 tcagcctcaa aaatctctcg tgctttaggg gtaccaatca acatcactgg tacgccaata
    10321 atattcacca tcgtcacaaa aaagttcagc atctcttgag atccacccga acgagagcgg
    10381 cttaaatgct gaatttcatc aataaccaac aaccctaaag catgtgcatt ggctatttgc
    10441 gacatcaaag ccaacatggt ttctatacca tgacgtttta agccataacg acgctcatag
    10501 ttcgagccca aggctcgatc caacgctctg aaaaaattca agcagatttc ttttagcgaa
    10561 ccattatgcg agcagtctat tttcaaatac accacctgct ctacattgag ttcacgatgg
    10621 taaatcacct gaggatacgt ggctagaata cgatgaagag aggtcgtctt cccactacca
    10681 gaacaaccaa ttaacaataa gctttgtgcc gtagatcgtg cctcctcaaa gcgaaatgtc
    10741 tccaactctc ccgtttgaac acgctcataa ccattttgta aatgcttttg taaatctcct
    10801 gttttaggat ttctgcctac gtagccacct cgaatcatga ccgaaatacg ctcacttagt
    10861 agcaaatgcg tacctaatgg ctgaaaatag tcatctggaa tacgacaaat ggtatgagct
    10921 ctgataacac gggacttttg caagtcagag gaagtaagct gtaaagagga tttcagtgat
    10981 gcagcactat tcactgactc ttgtaatggt ggtaaggcct cgataaaagg attatcacga
    11041 taagcctcta cccccgtatc acgataaact gcttgaatcc gggtagcact catgactcat
    11101 ccttttctgg tggatcctga aataattcag gcacgtatgt gggtaggctg taatcttctt
    11161 gatcatccgc ttccactgcg ttgaaaggaa taactttagc ctcatcacct gaagagcttg
    11221 gcttcaaatg ctccgcacgt tttttacgct ccgaggtcac ggcttctttt ttattagttt
    11281 taatctgctt aatgcgtgtt gatttgggct cagtagtact gggcgttaac ttattcgctt
    11341 tctgaatggt ttgctgaata aacgcctcaa gctccctgcg tttagttaac tcatcctgct
    11401 tcgcattggc tttattgtgt ttttcttgtg cttgtatatc ccaaacctcc caaaatgaga
    11461 gacctttaaa ctgccgacta cgttccgtca gattacagcg ccaaaataca cggctgccaa
    11521 cttgcggaaa caaataaatc gtatcaacca gcactgggtc ataagccgct tctaaatgtt
    11581 gaggtctagc tatatcagtg ctccgctgca accaaccctc acgcagaatc tctgaccccg
    11641 agtaatacaa accccacaaa ttaacgccaa atgaagaaat agagaccttt cggcgaggca
    11701 gtaacgctac tcgcaactgc tcttgctcca cagcccttaa actacctgta cgatgctgca
    11761 taccccattg ccatagctgg acaggaatag acggtaaatc tgtaggaaaa tcagcatctc
    11821 gatcgtattt atccatcacc agatggttat ttctgaataa gatcgtacgc aaaataattt
    11881 gtgtgaactc aaataccgac agagatgcat ctaacctata gtctgtttca ccatggcttt
    11941 tgatccgact gccctctaca atgccaggtg caaaggactt aaactcggct tgtagtgttc
    12001 taaaagtgct ttccactatg cctttagcat cgccacgtct aggtggagca ctttccactc
    12061 gcacattaaa actagaaact aaggcttcga cctgatgact cattaattcg ccacggtccg
    12121 ctagcaacac atctggcaaa cctacacacg gccagtctga gctactaatc tcaatatcat
    12181 gctgggcaca aatggccgtt ttgtcagagc aagcatttac aaaagcctgc atcgccacca
    12241 cataagacgg attttcaaag ccgatataaa agcccgtgat catccgacta aacacatcaa
    12301 tcacaatgta aagcgttggt cttcctatga ttttttggcg atcatgatga tccactaaat
    12361 aaatatcagc aatcgtggca tcaatctcat aacgactccc agggcctaac gcctgagaag
    12421 tggctgtact acttaagggt cgtacgtctt ttttatatac ccctgcttta actctagact
    12481 ttaagcgctg agctttaggg tattctcgat cataaaaata acgaaactga cgtagtgttg
    12541 ggtaatcctc ttggggaatg cgaggaaaat actgagcaaa caagtccaca aatcgtctat
    12601 aggcaacggt ggtctttgta cctttttgat ttaacaggtg cttttctatg gtcaacctaa
    12661 aaaggcgttc aatctcgggc gttaccttgg ttccttcacc cttaccatat tctctggctc
    12721 ggccaatctt tgctgttcct gtcgctgaac gtctttcccc tggtgcaccg ctgtttttgt
    12781 agtcaggaat taatgcatta ggcgtttgac cacgctgcca gtaacggcgt aacaacttat
    12841 aaactgtagc cttagtaacc ttatgttctt ggaccacatg ctcaacgagt tcgcttctga
    12901 ctttagggtc gaaacgatcc ttactattaa taataggaag aatttttcga taatcctcct
    12961 cgcgcttctg gaagctgaca gaatctacag acggctcttc taaatcaaga tgtacataag
    13021 ggtcatcagt tctaactaag cggccttcat ctaaatactg cattagcaac aactcagccc
    13081 tagcttgtgg aactccttta tcagcgctta tttgcatcca gacaacttgg ccatcctcta
    13141 tagccaaaat gcgatacgga tcattatcaa atagcacaac ctcattaatt tgccacatag
    13201 cgcaactcct ccatattcac tacttggcta atacagagat ctgcacactt atttgccctg
    13261 aaagacttat aaatattgaa cttaataaaa ccatttgcgg ttaaggctcg tatctcactc
    13321 aatgttttgc ctaactccaa atcataagca atatcaacct gcttacagac attgataatg
    13381 ttttcatctc ctttttcttg caggatatgg gccaatgggg atagttgtgc taaaagctcc
    13441 gcagaaactt cttctgtttt cactgaataa agccattcaa tattttcttt tactacggga
    13501 tttatttctt tatcagtaaa aatgaaccaa ggaatttgct tttgctgcca atagcgacgc
    13561 tctagttcta gtttttctaa ggtacgctcg tcttgtaagg ctgctgcagg tttgacttga
    13621 atagcaaact gctcaaaagg accatctttg cagtccacta aaaaatcagt agacataacc
    13681 tgatctacac cacgaataac aggatgctta ataccactat ctattgcaat ctgcctggta
    13741 tcactaggta ataaggggaa ctgctcgcgt atatctagca cgctgctctc ccactcaaga
    13801 ctgagaaaaa cagcaagctc taagtcagat agcaaatgat ggactcgtcc cgtcttatga
    13861 gaataaatac ggtgggaacg acctgaagaa ggaacttctt gtactgttag ccatggaata
    13921 tagtctttac catgcccttg gccacgcccc tctttaatac gacgggcaat ttgcacttca
    13981 gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc acagcataac
    14041 tggactgatt tcagtttaca actattctgt ctagtttaag acttgggtac cgagctcgaa
    14101 ttcgctagcc caaaaaaacg ggtatggaga aacagtagag agttgcgata aaaagcgtca
    14161 ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg tgacgccgtg
    14221 caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac gcgtttttgt
    14281 catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta ccggtttggt
    14341 tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat atggacaatt
    14401 ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa atgatcccct
    14461 gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga ttgaggccaa
    14521 cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata ttctcaatct
    14581 caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt gccgaccggg
    14641 tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc cggaggctcg
    14701 cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg aatggcttaa
    14761 ctggccgtca atatttgcca atacggggtt ctttcgcccg gatgaagcgc accagccgca
    14821 tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc gctattcgga
    14881 gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag cgattaacga
    14941 gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca tcagcgatca
    15001 cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct tgtcgccgtc
    15061 gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga
    15121 ccaacgtatc agccaggcga agctgctttt gagcaccacc cggatgccta tcgccaccgt
    15181 cggtcgcaat gttggttttg acgatcaact ctatttctcg cgggtattta aaaaatgcac
    15241 cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt
    15301 caagttgtca taattggtaa cgaatcagac aattgacggc ttgacggagt agcatagggt
    15361 ttgcagaatc cctgcttcgt ccatttgaca ggcacattat gcatcgatga taagctgtca
    15421 aacatgagca gatcctctac gccggacgca tcgtggcc
//
