LOCUS       sequence-226615-       11338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of late polyene biosynthetic genes in streptomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Stephens N, Rawlings B, Caffrey P
  TITLE     Versatility of enzymes catalyzing late steps in polyene 67-121C 
            biosynthesis.
  JOURNAL   Biosci Biotechnol Biochem. 2013;77(4):880-3. doi: 
            10.1271/bbb.120961. Epub 2013 Apr 7.
  PUBMED    23563553
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2013"; volume: "77(4):880-3. doi"; 
            pages: " 10.1271/bbb.120961. Epub 2013 Apr 7"
COMMENT     Sequence Label: pIAGO-AceF1
FEATURES             Location/Qualifiers
     source          1..11338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(124..146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     246..265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     459..481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     473..490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     474..490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4738..4754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4738..4754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4751..4773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4762..4778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4786..4792,4793..4810,4811..4816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4831..4852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9443..9460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9614..10202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9694..9713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10373..11164,11165..11233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10996..11015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11234..11338)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
      301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
      361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      481 gacggccagt gaattcgagc tcggtaccag cccgacccga gcacgcgccg gcacgcctgg
      541 tcgatgtcgg accggagttc gaggtacgcg gcttgcaggt ccaggaaggg gacgtccatg
      601 cgagtgtccg ttcgagtggc ggcttgcgcc cgatgctagt cgcggttgat cggcgatcgc
      661 aggtgcacgc ggtcgatctt gacggctggc gagaggtgcg gggaggatct gaccgacgcg
      721 gtccacacgt ggcaccgcga tgctgttgtg ggcacaatcg tgccggttgg taggatcctc
      781 tagagtcgac ctgcaggttc gtgggatcaa ggaagggtga acaccgaccc gcgcgccgtc
      841 atgttcgtca gcctgcccga aagcggactg ctgaacccga tgttggtgct cgccgccgag
      901 ctggcacgcc gcggcgtgcc aggcctgtcc ttcgccaccg acgagtcccg ccgtacggac
      961 atcgagacga tctccggcga ccacccggtg cgattcgcgt cactcggcga ggtcgtcccc
     1021 gagctgtccg ccgtcacctg ggacgacgcc acctaccgca gggtcaccca gcggtcgcgg
     1081 ttcaaggcgc atcgcgcggt catccggcac acgtaccggc cggaccttca ggcgaccaag
     1141 ttccgggctc tcgccgccgc ggtggacgag atccagccgg agctgatggt catcgagtcg
     1201 ctgtgcagtt tcgccgtcga cctcgccctg acccggggca tcccgttcgt gctcagcgtt
     1261 ccgttcctgc cgagcaatgt cctcaccgca cacaatccgt tcgccaggtc ctacacgccg
     1321 cgcggcttcc cggtgccgca ctccggcctg cccgcccgca tgacaccgtg gcagcggctg
     1381 agcaacgaga tgttcaagct gcgcaccctc gcgatgttct tccatccggc catgggcagg
     1441 gtcctttccg aggacgcccg gatccgcaag gagctcggcc tctccgtccc cagcccgatg
     1501 acgcgggtcg atcgcgccga gatggtcatc tgcaactcga tcccggagct ggactacccg
     1561 ttcgacatcc cggacaagct gaagttggtc ggcgcgctca tgccgccgct gcccgaggcc
     1621 tccacggaca acgaggtgat gcgctggctc gccgcccagc ggtcggtcgt ctacatgggt
     1681 ttcggcacga tcacccgcct cagcaccgcg caggccggtg cgctggtgga agtggctcga
     1741 cggctcgacg gcacgcattc ggtgctgtgg agactgccgt cggagcaaca gcacctcctg
     1801 gcggacgccg gtcccctgcc ggcgaacctg cgcatcgaga actggctgcc gtcgcaactg
     1861 gacgtgctcg cccatccggc ggtcaaggtg ttcttcaccc acggcggcgg caacggtttc
     1921 cacgagggcg tctacttcgg caagcccctg gtggtgcggc cgctctgggt ggactgcttc
     1981 gaccaggcga tccggggccg tgacgccggc gtgagcctga ccctggacca cccggagacc
     2041 gtcgatcccg acgacgtgct cgacaagctc aaccgggtcc tggacgaccc ctccttcact
     2101 gcccgcgccg ctcagctcgg cgctcgccag cgcgaggccg gcggtgtccg ggccgcggcc
     2161 gatctgatca accaggtggt gacgcgatga ccttcaagta ccccgtctcc atgccgacgc
     2221 tggacggacg cgagctcgag tacgtgacca acaccctcac cgacggctgg atctcctcac
     2281 aggggccggc cgtgggccgc ttcgagagcg ccttcgcgga gttcaacggc gtcgcgcacg
     2341 gcgtggcctg ctcctcgggc accgccgcgc tgaccctcgc gctgcgagcc ctgaacgtcg
     2401 ggccgggaga cgaggtcatc gtccccgagt tcaccatgat cgcctcggcg tgggcggtca
     2461 cctacaccgg cgccacgccg gtcttcgtgg actgcggcga cgacctgaac ctcgacgtca
     2521 ccctcatcga ggcggcgatc accccgcgga ccaaggtcat catgcccgta cacatctacg
     2581 gccggcgctg tgacatggac gcgatcatgg atctcgcgtt ccagtacaac ctgcgggtgg
     2641 tggaggactc cgccgaggcc catggagtcc gcccggtcgg cgacatcgcc tgcttctcgc
     2701 tgttcgccaa caagatcatc acttcgggtg agggcggcat ctgcgtgacc gagaacgccg
     2761 ccctggccgg tcagatggcc cacctccgcg ccatggcgtt cacccgggac cacagcttcc
     2821 tgcacaagaa gctggcctac aacttccgga tgaccgccat gcaggccgcc gtcgccctgg
     2881 cccagacgga acggttggac gacatcctgg cggcccgccg ggagatcgaa cggcgctacg
     2941 acgagggtct gcgcggcatc cccggcatca ccctcatgcc gcctcgcgat gtgctgtgga
     3001 tgtacgacgt ccgcgccgaa cgccgcgacg agctgcgggc gcacctgagc gccaacggca
     3061 tcgagacgcg cctgttcttc aagccgatga gccgccagcc cggctactac gacgagcgct
     3121 ggcaccacct gaacgcgacc cgcttcagcg aggacggctt ctacctgccc acgtacacgt
     3181 cattgagtgc caccgacatc gacaccatcg tggccgccgt ccgcgggttc tgcgagcggg
     3241 catgaccgag cgcaccgagt cgccaggaag gacgccggac gtgaccgact ccgaaccgct
     3301 ggtggtcgac tttccgctcc gcaaggaggg cgagtcgttt ccacccccgc ggtacgtgga
     3361 ataccgcgaa cgcgacgggc tggtgctgtc gtacctgccg gacggcaagc ccgtctggct
     3421 cgtcacccgg catgccgccg tccgggagat cctcaccagc aggaagatca gctccaatcc
     3481 ggaccgtgag ggcttccccc acatcggcga aacgatgggg gtgccgcgcc aggaccagat
     3541 ccccggctgg ttcgtcggcc tggatccgcc cgagcacgac cggttccgca aggcgctgat
     3601 cccggagttc accgtgcgcc ggatccgggc gatgcgcccg gcgatccagg gcgtcgtgga
     3661 caaggtcgtc gacgacctgc tcgcgggcgg ctcggaggcg gacctgatcg cggacttcgc
     3721 gctgccgatc ccgtcgctgg tcatctccgc gctgctcggt gtgccggtcg cggaccgcga
     3781 gttcttcgag tcacggaccc gcaccctgat cgccatccgc gcctcgaccg accagcagcg
     3841 cgacctggcc agccgggagc tgctgcgcta catcaaccgg ctgatcgaga tcaagtcgaa
     3901 gtggccgggc gacgatctgc ccagccggct gctggccggc ggcgcgctgg agccgcggga
     3961 gctgcccggt gtgctgctgc tcctgctgat cgccgggcac gagaccacgg ccaacaacat
     4021 cggcctgggc atcgtgacgc tgctggagaa cccccggtgg atcggcgacg accgcatcgt
     4081 cgaggaactg ctgcggctgc actcggtcgc cgacctggtg tcgctgcggg tggccgtcga
     4141 ggacgtcgag atctgcggtc agatgatcag ggcgggcgag ggcgtcgtgc cgctggtggc
     4201 cgcggccaac cacgacgaga cggtgttcgg ctgcccgcac ctgttcgatc ccggacgcag
     4261 cgcccggcag cacgtcgcgt tcggctacgg cgtgcaccag tgcctcgggc agaacctggt
     4321 ccgggtggag atggagctgg cgtaccggac gctgttcgag cggatccccg gactgcggct
     4381 cgcggtcggc gagcaaggcc tgccctacaa gtacgacgga gtcctgcacg gactgcacgc
     4441 actgccggtc cgctggtagg cctaccagcc acatccctgg aggacgagtt gcgtatcacg
     4501 gtgtcgaccc agacctgcgt cggcgcgggc cagtgcgtgc tgtccgcacc cgatgtcttc
     4561 gaccaggacg acgacggcgt ggtcgaggtg ctggacgccg agcccggcgc cggccacgcc
     4621 gaggcggtgt acctggcccg ggacctgtgc ccggccgggg cggtcactgt cgaggactag
     4681 caatccccgt gccggcgctc ggtttgtatg agcgacatca agcttggcgt aatcatggtc
     4741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4801 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagggaccgg cgccacaccg
     4921 gcttgcgccg gctcacggtc tcggacggcg cgaagccttc gcaagtacct tcgtgactac
     4981 ccggtggacg cgttctccat gctcaagggg atgcgcaagg cgatggaact catcggctac
     5041 ccgccgacgt ccacgagcgt tttcacagtc gaggcgatcg cggaaggcgt cagcgccatc
     5101 gagtcgttca agggcgtcgg ccgcgccttc gaggagtgcc cgcccggacc acgaccgttg
     5161 acctcgatat gtcagtggtg atcgctgccg tgtgaggtga tcatcactga cgaatcgagg
     5221 tcgaggaacc gagcgtccga ggaacagagg cgcttatcgg ttggccgcga gattcctgtc
     5281 gatcctctcg tgcagcgcga ttccgaggga aacggaaacg ttgagagact cggtctggct
     5341 catcatgggg atggaaaccg aggcggaaga cgcctcctcg aacaggtcgg aaggcccacc
     5401 cttttcgctg ccgaacagca aggccagccg atccggattg tccccgagtt ccttcacgga
     5461 aatgtcgcca tccgccttga gcgtcatcag ctgcataccg ctgtcccgaa tgaaggcgat
     5521 ggcctcctcg cgaccggaga gaacgacggg aagggagaag acgtaacctc ggctggccct
     5581 ttggagacgc cggtccgcga tgctggtgat gtcactgtcg accaggatga tccccgacgc
     5641 tccgagcgcg agcgacgtgc gtactatcgc gccgatgttc ccgacgatct tcaccccgtc
     5701 gagaacgacg acgtccccac gccggctcgc gatatcgccg aacctggccg ggcgagggac
     5761 gcgggcgatg ccgaatgtct tggccttccg ctcccccttg aacaactggt tgacgatcga
     5821 ggagtcgatg aggcggaccg gtatgttctg ccgcccgcac agatccagca actcagatgg
     5881 aaaaggactg ctgtcgctgc cgtagacctc gatgaactcc accccggccg cgatgctgtg
     5941 catgaggggc tcgacgtcct cgatcaacgt tgtctttatg ttggatcgcg acggcttggt
     6001 gacatcgatg atccgctgca ccgcgggatc ggacggattt gcgatggtgt ccaactcagt
     6061 catggtcgtc ctaccggctg ctgtgttcag tgacgcgatt cctggggtgt gacaccctac
     6121 gcgacgatgg cggatggctg ccctgaccgg caatcaccaa cgcaagggga agtcgtcgct
     6181 ctctggcaaa gctccccgct cttccccgtc cgggacccgc gcggtcgatc cccgcatatg
     6241 aagtattcgc cttgatcagt cccggtggac gcgccagcgg cccgccggag cgacggactc
     6301 cccgacctcg atcgtgtcgc cctgagcgtc cacgtagacg ttgcgtgaga gcaggactgg
     6361 gccgccgccg accgcaccgc cctcaccacc gaccgcgacc gcgccatggc cgccgccgac
     6421 ggcctggtcg ccgccgccgc ccgccggttc ggcgcctgac ccgaccaacc cccgcggcgc
     6481 ccccacccac caggagaccg accatgaccg acttcgacgg acgcctgacc gaggggaccg
     6541 tgaacctggt ccaggacccc aacggcggtg gctggtccgc ccactgcgct gagcccggtt
     6601 gcgactgggc cgacttcgcc ggaccgctcg gcttccaggg cctcgtggcc atcgctcgcc
     6661 gacacacgca ctgaccgcac gtcaaagccc cgccggatca ccggcggggc tctcttcggc
     6721 cctccaagtc acaccagccc caaggggcgt cgggagtggc ggagggaacc tctggcccga
     6781 ttggtgccag gattcccacc agaccaaaga gcaacgggcc ggacttcgca cctccgaccc
     6841 gtccgctccc agactcgcgc cccttagccg ggcgagacag gaacgttgct cgtgcccaga
     6901 gtacggagcg atgccgaggc attgccagat cggcccgccg ggccccgctg ccactgcggg
     6961 accgcaattg cccacacacc gggcaaacgg ccgcgtatct actgctcaga ccgctgccgg
     7021 atggcagcga agcgggcgat cgcgcgtgtg acgcgagatg ccgcccgagg caaaagcgaa
     7081 caccttggga aagaaacaac agagtttccc gcacccctcc gacctgcggt ttctccggac
     7141 ggggtggatg gggagagccc gagaggcgac agcctctcgg aagtaggaag cacgtcgcgg
     7201 agcgacgctg cccgactgcg gaaagccgcc cggtacagcc gccgccggac gctgtggcgg
     7261 atcagcgggg acgccgcgtg caagggctgc ggccgcgccc tgatggaccc tgcctccggc
     7321 gtgatcgtcg cccagacggc ggccggaacg tccgtggtcc tgggcctgat gcggtgcggg
     7381 cggatctggc tctgcccggt ctgcgccgcc acgatccggc acaagcgggc cgaggagatc
     7441 accgccgccg tggtcgagtg gatcaagcgc ggggggaccg cctacctggt caccttcacg
     7501 gcccgccatg ggcacacgga ccggctcgcg gacctcatgg acgccctcca gggcacccgg
     7561 aagacgccgg acagcccccg gcggccgggc gcctaccagc gactgatcac gggcggcacg
     7621 tgggccggac gccgggccaa ggacgggcac cgggccgccg accgcgaggg catccgagac
     7681 cggatcgggt acgtcggcat gatccgcgcg accgaagtca ccgtggggca gatcaacggc
     7741 tggcacccgc acatccacgc gatcgtcctg gtcggcggcc ggaccgaggg ggagcggtcc
     7801 gcgaagcaga tcgtcgccac cttcgagccg accggcgccg cgctcgacga gtggcagggg
     7861 cactggcggt ccgtgtggac cgccgccctg cgcaaggtca accccgcctt cacgcccgac
     7921 gaccggcacg gcgtcgactt caagcggctg gagaccgagc gcgacgccaa cgacctcgcc
     7981 gagtacatcg ccaagaccca ggacgggaag gcgcccgccc tcgaactcgc ccgcgccgac
     8041 ctcaagacgg cgaccggcgg gaacgtcgcc ccgttcgaac tcctcggacg gatcggggac
     8101 ctgaccggcg gcatgaccga ggacgacgcc gccggggtcg gctcgctgga gtggaacctc
     8161 tcgcgctggc acgagtacga gcgggcaacc cggggacgcc gggccatcga atggacccgc
     8221 tacctgcggc agatgctcgg gctcgacggc ggcgacaccg aggccgacga cctcgatctg
     8281 ctcctggcgg ccgacgccga cggcggggag ctgcgggccg gggtcgccgt gaccgaggac
     8341 ggatggcacg cggtcacccg ccgcgccctc gacctcgagg cgacccgggc cgccgaaggc
     8401 aaggacggca acgaggattc ggcggccgtg ggcgaacggg tgcgggaggt cctggcgctg
     8461 gccgacgcgg ccgacacagt ggtggtgctc acggcggggg aggtggccga ggcgtacgcc
     8521 gacatgctcg ccgccctcgc ccagcgccgc gaggaagcaa ctgcacgccg acggcgagag
     8581 caggacgacg accaggacga cgacgccgac gaccgccagg agcgggccgc ccggcacatc
     8641 gcccggctcg caagtgggcc cacttcgcac taactcgctc ccccccgccg tacgtcatcc
     8701 cggtgacgta cggcgggggt cggtgacgta cgcggcgacg gcggccgggg tcgaagccgc
     8761 gggagtaatc ctgggattac tcgcccgggg tcggccccgc cggcacttcg tgcaggcggt
     8821 acctcgcgcc cgactcgcct cgctacgaga cgtgccgcgt acggtcgtcg gccatgagca
     8881 ccaccacccc cagggacgcc gacggcgcga agctctgcgc ctggtgcggc tcggagatca
     8941 agcaatccgg cgtcggccgg agccgggact actgccgccg ctcctgccgc cagcgggcgt
     9001 acgaggcccg gcgccagcgc gaggcgatcg tgtccgccgt ggcgtcggca gtcgctcgcc
     9061 gagatacgtc acgtgacgaa atgcagcagc cttccattcc gtcacgtgac gaaactcggg
     9121 ccgcaggtca gagcacggtt ccgcccgctc cgtcgtggcc gtagtacgag acgtcggtga
     9181 ccgttgccct gaccgcgccg acggagtcct cgccggtgag gcggagttgt tccggacgga
     9241 gcaggaccgt gccggcgcgc aggccgccgg gcggagcggc gaggggcacc gtgcccaagg
     9301 cggtccgggc cgtgtcgccg gagtcgacgt cgacggtgcc ggggagcagg acggcgtcgc
     9361 cgacgaagcc ggcgaccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9541 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9841 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10321 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10381 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10441 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10501 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10561 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10621 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10681 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10741 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10801 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10861 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10921 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10981 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11041 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11101 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11161 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11221 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11281 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcg
//
