LOCUS       sequence-226651-        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      535..544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1048..1100
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1108..1117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1114..1827
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     polyA_signal    1949..2070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1986..2005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2040..2059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2077..2532)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2214..2235)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2426..2445
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2559..2663
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2665..3022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2665..2856
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2688..2708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2873..3008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3057..3851
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3057..3851
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3111..3130)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3721..3740
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4039..4058)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4083..4130
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4459..5047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4948..4967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      541 atgggcccca agcggcggca gctgacgttc cgggagaagt cgcggatcat ccaggaggtg
      601 gaggagaacc cggacctacg caagggcgag atcgcgcggc gcttcaacat cccgccgtcc
      661 acgctgagca cgatcctgaa gaacaagcgc gccatcctgg cgtcggagcg caagtacggg
      721 gtggcctcca cctgccgcaa gaccaacaag ctgtctccct acgacaagct cgagggcttg
      781 ctcatcgcct ggttccagca gatccgcgcc gccggcctgc cggtcaaggg catcatcctc
      841 aaggagaagg cgctgcgcat agccgaggag ctgggcatgg acgacttcac cgcctccaac
      901 ggctggctgg accgcttccg ccggcgccac ggcgtggtgt cctgcagcgg cgtggcccgc
      961 gcccgcgcgc gaaacgctgc cccccgcacc ccggcggcgc ctgccagtcc ggccgcggtg
     1021 ccctcggagg gcagtggcgg gagcactctc gagctcaagc ttcgaattct gcagtcgacg
     1081 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggggcaatcg
     1141 aagcatgggc taaaggagga gatgacggtg aagtaccaca tggaaggttg cgttaatggt
     1201 cacaaatttg tcattactgg agagggcatt ggaaaccctt ttaagggtaa gcaaactgca
     1261 aatttgtgtg tgatagaagg aggcccgctg ccgttctcgg aggacatttt aagtccgggt
     1321 tttaaatatg gtgaccggat tttcacagag tacccacagg atattgtaga ttacttcaag
     1381 aactcatgtc cagcgggcta tacgtgggaa aggagcttcc tctttgagga cggagcggtc
     1441 tgtcgatgca acgtggacat aacagtctct gaaaaggaga actgcatcta tcacaaaagt
     1501 atcttcagag gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat gaccactaat
     1561 tgggaagcta gtaccgagaa aattgtgcct gttccaaagc aagggatatt aaaaggaaag
     1621 gtcaaaatgt acctattgct gaaggatggc ggtcgttatc attgccagtt tgatacggta
     1681 tataaagcta aatcagtgcc ctccaagatg ccagaatggc attttataca gcataagctc
     1741 cttagggagg accgctctga tgctaaaaac cagaaatggc aattaacaga acatgcaatc
     1801 gcaggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     1861 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1921 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2041 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     2101 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2161 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2221 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2281 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2341 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2401 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2461 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2521 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2581 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2641 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2701 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2761 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2821 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2941 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3001 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     3061 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3121 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3181 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3241 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3301 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3361 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3421 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3481 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3541 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3601 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3661 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3721 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3781 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3841 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3901 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3961 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4021 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4081 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     4141 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4201 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4261 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4321 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4381 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4561 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     5161 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5221 g
//
