LOCUS       sequence-226739-        5763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa169 to C-terminus of Flp recombinase fused to FKBP CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2277_pCAG-PV1-FKBP-L1-FlpO-169C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             686..1006
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1814..1834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1844..2068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2308..2327)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2373..2428
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2427..2446)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2789..2805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2789..2805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2802..2824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2813..2829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2837..2843,2844..2861,2862..2867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2882..2903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2988..3122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3142..3276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3179..3198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3233..3252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3344..3361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3515..4103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3595..3614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4274..5065,5066..5134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4897..4916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5135..5239)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5270..5649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ctagaggagt gcaggtggaa accatctccc caggagacgg
      721 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
      781 tggaaagaaa tttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
      841 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
      901 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
      961 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatccg gagggtctgg
     1021 ctccggatca agtggtggca gcggtaccag caggttcacc aagaccaaga ccctgtacca
     1081 gttcctgttc ctggccacat tcatcaactg cggcaggttc agcgacatca agaacgtgga
     1141 ccccaagagc ttcaagctgg tgcagaacaa gtacctgggc gtgatcattc agtgcctggt
     1201 gaccgagacc aagacaagcg tgtccaggca catctacttt ttcagcgcca gaggcaggat
     1261 cgaccccctg gtgtacctgg acgagttcct gaggaacagc gagcccgtgc tgaagagagt
     1321 gaacaggacc ggcaacagca gcagcaacaa gcaggagtac cagctgctga aggacaacct
     1381 ggtgcgcagc tacaacaagg ccctgaagaa gaacgccccc taccccatct tcgctatcaa
     1441 gaacggccct aagagccaca tcggcaggca cctgatgacc agctttctga gcatgaaggg
     1501 cctgaccgag ctgacaaacg tggtgggcaa ctggagcgac aagagggcct ccgccgtggc
     1561 caggaccacc tacacccacc agatcaccgc catccccgac cactacttcg ccctggtgtc
     1621 caggtactac gcctacgacc ccatcagcaa ggagatgatc gccctgaagg acgagaccaa
     1681 ccccatcgag gagtggcagc acatcgagca gctgaagggc agcgccgagg gcagcatcag
     1741 ataccccgcc tggaacggca tcatcagcca ggaggtgctg gactacctga gcagctacat
     1801 caacaggcgg atccccaaga aaaagcggaa ggtgtgagaa ttcctgtgcc ttctagttgc
     1861 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1921 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1981 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2041 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     2101 agggggtatc cccacgcgcc ctgtagcgtg taccgctggg agttcgtaga cggaaacaaa
     2161 cgcagaatcc aagcgcggcc gcccaggata catagattac cacaactccg agcccttcca
     2221 ccgctagcgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     2281 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     2341 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     2401 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2461 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     2521 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     2581 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     2641 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     2701 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     2761 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2941 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3121 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3241 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     3301 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     5281 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5341 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5401 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5461 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5521 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5581 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5641 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     5701 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     5761 ggg
//
