LOCUS       sequence-226771-        5760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa429 to C-terminus of PhiC31 integrase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1847..2071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2311..2330)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2376..2431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2430..2449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2792..2808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2792..2808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2805..2827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2816..2832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2840..2846,2847..2864,2865..2870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2885..2906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2991..3125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3145..3279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3182..3201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3236..3255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3347..3364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3518..4106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3598..3617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4277..5068,5069..5137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4900..4919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5138..5242)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5273..5652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtaccggcga cgaggagacc ctggccctgc tgtgggaggc
     1321 cgccagaaga ttcggcaagc tgaccgaggc ccccgagaag agcggcgaga gggccaacct
     1381 ggtggccgag agagccgacg ccctgaacgc cctggaggag ctgtacgagg acagagccgc
     1441 cggagcctat gacggccctg tgggcaggaa gcacttcaga aagcagcagg ccgccctgac
     1501 cctgagacag cagggcgccg aggaaagact ggccgagctg gaggccgccg aggcccctaa
     1561 gctgcccctg gatcagtggt tccccgagga tgccgacgcc gaccccaccg gccccaagtc
     1621 ctggtggggc agagccagcg tggacgacaa gagggtgttc gtgggcctgt tcgtggataa
     1681 gatcgtggtg accaagagca ccaccggcag gggccagggc acccccatcg agaagagagc
     1741 cagcatcacc tgggccaagc ctcccaccga cgacgacgag gatgacgccc aggacggcac
     1801 cgaggacgtg gccgccccca agaaaaagcg gaaggtgtga gaattcctgt gccttctagt
     1861 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1921 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     1981 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2041 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     2101 tctagggggt atccccacgc gccctgtagc gtgtaccgct gggagttcgt agacggaaac
     2161 aaacgcagaa tccaagcgcg gccgcccagg atacatagat taccacaact ccgagccctt
     2221 ccaccgctag cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2281 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2341 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     2401 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2461 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     2521 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     2581 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     2641 tgtgttattt ttttctttaa catccctaaa atttttctta catgttttac tagccagatt
     2701 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     2761 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2821 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2881 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2941 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     3001 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3061 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3121 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     3181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3241 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     3301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4321 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4381 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4441 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4501 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4561 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4621 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4681 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4741 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4801 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4861 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4921 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4981 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5041 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5101 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5161 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5221 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     5281 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5641 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     5701 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
//
