LOCUS       sequence-226876-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gRNA vs Dmap1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, 
            Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
  TITLE     CRISPR-KO screen identifies Dmap1 as a regulator of 
            chemically-induced reprogramming and differentiation of cardiac 
            progenitors.
  JOURNAL   Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012.
  PUBMED    30932271
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2019 Apr 1. doi: 10.1002/stem.3012."
COMMENT     Sequence Label: pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        146..344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     299..319
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             362..542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    589..714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1213..1446
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1631..1675
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1973..2090
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2165..2413
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2165..2185
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2336..2355
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2541..3040
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2576..2599)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2973..2992
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3080..3676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3080..3099)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3576..3596
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3695..3748
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3749..4450
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4510..4530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4928..4939)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5391..5408)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5397..5621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5647..5667)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5647..5665)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5686..5702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5686..5702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5699..5721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5710..5726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5734..5740,5741..5758,5759..5764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5779..5800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5917..5934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6088..6676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6168..6187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6847..7638,7639..7707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7470..7489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7708..7812)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7838..8293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7975..7996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8187..8206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8420..8442
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8434..8451
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8435..8451
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8461..8480
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8461..8479
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
       61 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      121 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      181 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      241 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      301 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
      361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
      541 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
      601 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
      661 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
      721 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
      781 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
      841 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
      901 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
      961 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1021 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gcgctgatct tcagacctgg
     1081 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1141 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1201 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1261 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1321 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1381 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1441 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1501 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1561 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1621 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1681 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1741 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1801 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1861 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1921 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1981 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2041 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2101 acagggacag cagagatcca gtttggttag taccgggccc tacgcgtcca aggtcgggca
     2161 ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     2221 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     2281 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     2341 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     2401 aggacgaaac accgaagcgc agatcaaatc gggtttaaga gctatgctgg aaacagcata
     2461 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     2521 tttttggatc caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     2581 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     2641 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     2701 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2761 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2821 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2881 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
     3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3661 ccgcaagccc ggtgccggcg gcgggtccgg aggagagggc agaggaagtc tcctaacatg
     3721 cggtgacgtg gaggagaatc ctggcccaat gagcgagctg attaaggaga acatgcacat
     3781 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     3841 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     3901 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3961 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     4021 gagagtcacc acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca
     4081 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     4141 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     4201 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     4261 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     4321 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     4381 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     4441 gcttaattga gcggccgctc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4501 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4561 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4621 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4681 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4741 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4801 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4861 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4921 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4981 gcctcttccg cgtctacgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5041 cccgcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5101 aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagtcaag atctgctttt
     5161 tgcctgtact gggtctctct ggttagacca gagtctctct ggttagacca gatctgagcc
     5221 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5281 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5341 cccttttagt cagtgtggaa aatctctagc agtttaaacc cgctgatcag cctcgactgt
     5401 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5461 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5521 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5581 agtcaatagc aggcatgctg gggatgcggt gggctctatg ggcggccgtt aattaagctt
     5641 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     5701 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
     5761 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5821 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5881 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6601 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6661 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6721 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6781 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6841 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6901 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6961 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7021 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7081 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7141 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7201 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7261 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7321 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7381 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7441 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7501 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7561 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7621 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7681 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7741 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7801 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     7861 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7921 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     7981 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     8041 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     8101 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8161 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8221 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8281 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8341 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8401 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     8461 taatacgact cactataggg cgaattgact agtacataac ttacggtaaa tggcccgcct
     8521 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8581 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8641 ttggcagta
//
