LOCUS       sequence-22689-e         392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Gu P, LeMenuet D, Chung AC, Mancini M, Wheeler DA, Cooney AJ
  TITLE     Orphan nuclear receptor GCNF is required for the repression of 
            pluripotency genes during retinoic acid-induced embryonic stem cell 
            differentiation.
  JOURNAL   Mol Cell Biol. 2005 Oct . 25(19):8507-19.
  PUBMED    16166633
REFERENCE   2  (bases 1 to 392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Oct . 25(19):8507-19."
COMMENT     Sequence Label: pCMV-HA GCNF
FEATURES             Location/Qualifiers
     source          1..392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          142..238
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             302..328
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact
      121 gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga
      181 tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc
      241 ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat
      301 gtacccatac gatgttccag attacgctct tatggccatg gaggcccgaa ttcgcatgga
      361 gcgggacgaa cggccaccta gcggaggggg ag
//
