LOCUS       sequence-227069-        9295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter,
            encodes FsCRISPR array 2, compatible with SENECA acquisition 
            readout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Schmidt F, Cherepkova MY, Platt RJ
  TITLE     Transcriptional recording by CRISPR spacer acquisition from RNA.
  JOURNAL   Nature. 2018 Oct;562(7727):380-385. doi: 10.1038/s41586-018-0569-1. 
            Epub 2018 Oct 3.
  PUBMED    30283135
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0569-1"; journalName: "Nature"; date: "2018-10";
            volume: "562"; issue: "7727"; pages: "380-385"
COMMENT     Sequence Label: 
            pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_s
            accharivorans_ARRAY2_FaqI
FEATURES             Location/Qualifiers
     source          1..9295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(210..1025)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(210..1025)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     933..952
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1017..1036
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1147..1735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1636..1655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1889..1906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1921..2060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1986..2005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2146..2168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2162..2353)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3162..4244)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4206..4225
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4245..4322)
                     /gene="lacI"
                     /label=lacI promoter
     RBS             4476..4487
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4494..5117
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4567..4586)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      5162..5233
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5249..5276
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    5291..5309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5316..5334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5373..6308
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      6330..6416
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6534..6553
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6641..6660
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6641..6659
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6660..6684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6699..6721
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(8974..8992)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8988..9035
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
      241 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
      301 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
      361 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
      421 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
      481 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
      541 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
      601 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
      661 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
      721 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
      781 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
      841 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
      901 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
      961 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     1021 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
     1081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1741 cagcaacgcg gcctttttac ggttcctggt cttttgctgg ccttttgctc acatgttctt
     1801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     1981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2041 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     2221 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     2281 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     2341 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     2401 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     2461 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     2521 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     2581 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     2641 gactttacga aacacggaaa ccgaagatca ttcatgttgt tgctcaggtc gcagacgttt
     2701 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     2761 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     2821 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     2881 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     2941 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     3001 gtcctacgag ttgcatgata aagaagatag tcataagtgc ggcgacgata gtcatgcccc
     3061 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     3121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3241 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     3301 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     3361 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct atcttcggta
     3421 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
     3481 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     3541 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     3601 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     3661 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     3721 accagatgct ccacgcccag tcgcgtacca tcttcatggg agaaaataat actgttgatg
     3781 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     3841 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     3901 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3961 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4021 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     4081 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     4141 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     4201 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     4261 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     4321 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     4381 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaccggtg gcacgtaaga
     4441 ggttccaact ttcaccataa tgaaacatac tagagaaaga ggagaaatac tagatgtcca
     4501 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     4561 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     4621 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
     4681 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     4741 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     4801 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     4861 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     4921 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     4981 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     5041 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     5101 gtgaaagtgg gtcctaataa cactgatagt gctagtgtag atcactacta gagccaggca
     5161 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     5221 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     5281 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
     5341 gcactactag agaagcttgc ggaggagcat gcatggcttc caaggtgtac gaccccgagc
     5401 aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc
     5461 tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc
     5521 tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg
     5581 tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg
     5641 gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc
     5701 ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact
     5761 cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga
     5821 tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag
     5881 agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga
     5941 tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg
     6001 gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca
     6061 agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc
     6121 tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag
     6181 ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg
     6241 acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg
     6301 agcagtaatg agatcggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     6361 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgg
     6421 atcccatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     6481 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     6541 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     6601 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg
     6661 gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag
     6721 atatacatat gttcactata gacgagatgc tatcaaagaa caaccaaaga ttggctttcg
     6781 agcacttcgc tactaagaac gacggatgtg gccctgatgg tatgcacgta tctgagctgg
     6841 agaaatattg gcgtatgaat cacgatcaga tcatctccga cttgaagaac caagagtacc
     6901 aacccggcat tattttgatt agagaacaca tgaacaaaac aggtaagaga aggaacattg
     6961 catctctcaa cgtgatcgac aggttcataa ctcgactgct gtcgcaaaag ttgaatagat
     7021 acttagcccc cattttctgc gagaactcat acgcttacca agactcaaaa ggggtgatgc
     7081 ccgccgtact aaaagccaag gagtacgtcg agttgggtat gagacacgtt atcgagatag
     7141 acctcaagaa ttacttcgac acgatcccgt tagagaactt aatcccagag atcgagcgtt
     7201 acataacaga tgaggcagtg ctgcacttga ttaaacagta cttattctgt gacatctcgt
     7261 tcgagggtaa gatatcacgt aaaactcaag ggatcgttca gggtaacgca atttccccaa
     7321 tactatccaa cctctacctg aacgacttcg acaaggagct ggacgagtcg aagctgtgct
     7381 ggatcagata cgccgacaac atatacatct atatggacag ctacgagaag gccctactgg
     7441 tttattccga gcttaccgag agacttgaga gaaggaaatt aaccgttaac aaggagaaat
     7501 caggggtgtt tgatgttagt acccgttcta tactaggtta cgacatcctg atcagaaaca
     7561 agaaggttga cgtgcgtaaa cacatctaca agtctgttaa ccagtactcc aactggcacg
     7621 actctcgatt ggagttcatc aacggtcgtt accacatcac ttccgacggt atcctcaata
     7681 gacaagactt cggtctactg ttcgagaatg agcagaagaa gcactacata cctgttgagg
     7741 tttctgacca gttgaatatt tacggaaacg ttacgctcgc cagcaacgtt cttcaatcat
     7801 tctccaaccg agagatcaaa gtgtccttct tcgacaagta cgggagactt attggatctt
     7861 tcctgcccga gaaaactaag aaaagcgccg agataatact ggttcagtct aagaactacc
     7921 ttaacgagga cgttaggatg gacacggccc gacgtatgga gatcgccggt ttgcataaca
     7981 tcagagccaa cctcagatac tacgacaaga agcacaaagg tgactttaag gaaaaggtag
     8041 acgccatttc tggctatatt gacgccctca atagagcacc atccgtgaac gacatgatgc
     8101 tcttagaggc taaagctcgt cagctgtact acacatgctt caaccagatc ttagagacat
     8161 ccgactttca gttcgagaag cgtaccaagc gaccaccaaa agacgccatc aacgcctgta
     8221 tttcattcgg caacacatta ctgtacaact tgtttgtgaa tatcatttgg aagaagggac
     8281 ttgatccaag gttcggggtt gttcacgcca gcaacaagcg taatcagtcc cttaacctgg
     8341 attttgctga catcttcaag cctatagtca tagataggat catcttcaca atgatcaaca
     8401 agaagatgct gaccttgttg acggacttcg agacttctaa ccagggagtt tacctttccc
     8461 gagaggggaa gaacatcttc ttgcagatgt acgaagaaaa gctcaaatcc agaatcacta
     8521 tcaagggaaa ggagatgagt tactatcagt tgctagaatc agaggtgcag aactacaaga
     8581 acttcatcct gaccggcgag acatacaagc cgtacaagta ctactagcgt acgttaactt
     8641 taagaaggag aacttaagat gtatgtcatt ctagtttacg acattcacca gaagcgagta
     8701 ggtaaggctc taaagatttg taggaagtac ctgatccaca tccagaagtc agttttcgaa
     8761 ggcaacataa cggagagtaa gctaaaggcc ttaaaagagg agctggggca cctgatcgac
     8821 actcagatgg actcggtaat tatctaccac ctggactccg tgaagtacac taagaaggag
     8881 cagatcggca tcgtccaatc gacatccaat gtcatttgac tcgagatccg gctgctaaca
     8941 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     9001 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     9061 accaggtgcg gggaagctta tgttccatag caaaaagtcg gtcagtctcg tggctgaaat
     9121 catgagttcc acaaaatggc tgaaattcaa ggaaaatcag gaatctcaga aaaacgatcg
     9181 accgactttt tcgataaaat ggttgcaaaa atgagaaaaa tctgatttaa tagaatctga
     9241 aaacagcgga aatgctgttg tcgtacttta cctaaaagga attgaaacgt cccgc
//
