LOCUS       sequence-227077-        7757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn5 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn5-166
FEATURES             Location/Qualifiers
     source          1..7757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1617..1711
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1755..2414
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1821..1840)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2479..2565
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4309..4327)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4395..4499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4500..4568,4569..5360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4718..4737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            5599..5708
                     /note="incP origin of transfer"
     primer_bind     6262..6278
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6262..6278
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(6341..7540)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
       61 ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
      121 ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
      181 gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
      241 gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
      301 gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
      361 gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
      421 tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
      481 tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gctaagaaac
      541 cattattatc atgaagctcg cacgctgccg caagcactca gggcgcaagg gctgctaaag
      601 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
      661 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
      721 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
      781 gccagctggg gcgccctctg gtaaggttgg gaatccctgc aaagtaaact ggatggcttt
      841 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatacta
      901 gtggaggaag aaaaaatggt gagcaagggc gaggagctga tcaaggagaa catgcacatg
      961 aagctgtaca tggaaggcac cgtgaacaac caccacttca agtgcaccac cgaaggggag
     1021 ggcaagccct acgagggcac ccagacccag aggattaagg tggtggaggg aggccccctg
     1081 ccgttcgcat tcgacatcct ggccacctgc tttatgtacg ggagcaagac cttcatcaac
     1141 cacacccagg gcatccccga tttctttaag cagtccttcc ctgagggctt cacatgggag
     1201 agagtcacca catacgaaga cgggggcgtg cttaccgtta cccaggacac cagcctccag
     1261 gacggctgct tgatctacaa cgtcaagctc agaggggtga acttcccatc caacggccct
     1321 gtgatgcaga agaaaacact cggctgggag gccaccaccg agaccctgta ccccgctgac
     1381 ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg tgggcggggg ccacctgcac
     1441 tgcaacctga agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     1501 gtctactttg tggaccgcag actggaaaga atcaaggagg ccgacaatga gacctacgtc
     1561 gagcagcacg aggtggctgt ggccagatac tgcgacctcc taattagctg agcttggact
     1621 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     1681 ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga ttttcaggag
     1741 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     1801 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1861 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1921 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     1981 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     2041 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     2101 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     2161 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     2221 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     2281 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     2341 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     2401 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
     2461 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2521 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctaggagct
     2581 tgcggccgcc aggatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
     2641 ggcttcccgg ccgacgcgtc aattcgagcg cgtcaattcg agggccgcgg cctaggcggc
     2701 cagatctgat caagagacag tctagctaga attaattcga gtcgaccgat cccgtacaca
     2761 agtagcgtcc tgaacggaac ctttcccgtt ttccaggatc tgatcttcca tgtgacctcc
     2821 taacatggta acgttcatga taacttctgc tcttcatcgt gcggccgact gggctaaatc
     2881 tgtgttctct tcggcggcgc tgggtgatcc tcgccgtact gcccgcttgg ttaacgtcgc
     2941 cgcccaattg gcaaaatatt ctggtaaatc aataaccatc tcatcagagg gtagtgaagc
     3001 catgcaggaa ggcgcttacc gatttatccg caatcccaac gtttctgccg aggcgatcag
     3061 aaaggctggc gccatgcaaa cagtcaagtt ggctcaggag tttcccgaac tgctggccat
     3121 tgaggacacc acctctttga gttatcgcca ccaggtcgcc gaagagcttg gcaagctggg
     3181 ctctattcag gataaatccc gcggatggtg ggttcactcc gttctcttgc tcgaggccac
     3241 cacattccgc accgtaggat tactgcatca ggagtggtgg atgcgcccgg atgaccctgc
     3301 cgatgcggat gaaaaggaga gtggcaaatg gctggcagcc gccgcaacta gccggttacg
     3361 catgggcagc atgatgagca acgtgattgc ggtctgtgac cgcgaagccg atattcatgc
     3421 ttatctgcag gacaaactgg cgcataacga gcgcttcgtg gtgcgctcca agcacccacg
     3481 caaggacgta gagtctgggt tgtatctgta cgaccatctg aagaaccaac cggagttggg
     3541 tggctatcag atcagcattc cgcaaaaggg cgtggtggat aaacgcggta aacgtaaaaa
     3601 tcgaccagcc cgcaaggcga gcttgagcct gcgcagtggg cgcatcacgc taaaacaggg
     3661 gaatatcacg ctcaacgcgg tgctggccga ggagattaac ccgcccaagg gtgagacccc
     3721 gttgaaatgg ttgttgctga ccagcgaacc ggtcgagtcg ctagcccaag ccttgcgcgt
     3781 catcgacatt tatacccatc gctggcggat cgaggagttc cataaggcat ggaaaaccgg
     3841 agcaggagcc gagaggcaac gcatggagga gccggataat ctggagcgga tggtctcgat
     3901 cctctcgttt gttgcggtca ggctgttaca gctcagagaa agcttcacgc tgccgcaagc
     3961 actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac
     4021 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg
     4081 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt
     4141 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctgggaag gttgggaagc
     4201 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
     4261 gatctgatcg gtcgatcgaa ttaattccca attcttgaag acgaaagggc ctcgtgatac
     4321 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4501 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4561 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4621 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4681 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4741 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4801 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4861 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4921 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4981 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5041 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5101 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5161 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5221 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5281 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5341 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5401 taaaacttca tttttaattt aaaaggatct aggtgaagat ccggggaatt ggggatcaat
     5461 tccgttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     5521 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     5581 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     5641 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     5701 ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc
     5761 aaccaggaag ggcagcccac ctatcacgga attgatccgg ccacgatgcg tccggcgtag
     5821 aggatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct catgtttcac
     5881 gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg
     5941 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
     6001 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
     6061 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatgta
     6121 acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag gaacacttaa
     6181 cggctgacat gggaattaat tctgactctt atacacaagt gcggccctcg aattgacgcg
     6241 ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat
     6301 atccataaac tgccaggaat tggggatcgg attcgagctc tcagcgatcg gctcgttgcc
     6361 ctgcgccgct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag agggcagcgc
     6421 ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc gccgtgaaga
     6481 ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct tgcagctgcc
     6541 cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc attccgatgc
     6601 cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc gcgaaggcaa
     6661 gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc ctttcgccga
     6721 gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga atgccaaatg
     6781 cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg ccgaaaatga
     6841 cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc gccatcaggg
     6901 cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg agagcctccc
     6961 ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag cccgtcagga
     7021 aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag aaaccgccca
     7081 tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg ctcatgaagc
     7141 cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg ccggctaccg
     7201 ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc caaaggaaag
     7261 gcgctgtcgc catgatggcg tagtcgacag tggcgccggc cagcgagacg agcaagattg
     7321 gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca
     7381 acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat
     7441 cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga
     7501 cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc
     7561 tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat
     7621 gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc
     7681 cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga
     7741 acggttgggg gttcagc
//
