LOCUS       sequence-227255-         103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optimized aTc inducible pTet promoter for Rhodococcus opacus 
            expressing mCherry on pAL5000/pMB1 backbone with kanamycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 103)
  AUTHORS   DeLorenzo DM, Henson WR, Moon TS
  TITLE     Development of Chemical and Metabolite Sensors for Rhodococcus 
            opacus PD630.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1973-1978. doi: 
            10.1021/acssynbio.7b00192. Epub 2017 Aug 10.
  PUBMED    28745867
REFERENCE   2  (bases 1 to 103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00192"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1973-1978"
COMMENT     Sequence Label: pDD131
FEATURES             Location/Qualifiers
     source          1..103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tctgaccagg gaaaatagcc ctctgacctg gggatttgac ttccctatca gtgatagaga
       61 taatctggga gtccctatca gtgatagaga aggcggtatc gat
//
