LOCUS       sequence-227317-       18664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 
            cassette to the mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 18664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector
FEATURES             Location/Qualifiers
     source          1..18664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2975..3056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3000..3019
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    join(3070..3088,3089..3111,3112..3130,3131..3153,3154..3172,
                     3173..3195,3196..3214,3215..3237,3238..3256,3257..3279,
                     3280..3298,3299..3321,3322..3340)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3373..3411
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3408..3432
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3545..3578)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3655..3703
                     /note="synthetic polyadenylation signal"
     polyA_signal    3712..4188
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    4200..4661
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4688..4721)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4732..4741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4738..4746,4747..4764,4765..4767,4768..4800,4801..4803,
                     4804..4827,4828..4854,4855..4911,4912..4917,4918..5187,
                     5188..5205,5206..5640,5641..5646,5647..6090)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4834..4843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5124..5145
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5251..5272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5512..5531)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6115..6703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6586..6597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6734..6941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9465..9768
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9770..10047
                     /label=chicken beta-actin promoter
     intron          10048..11064
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10988..11010
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11072..11091
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    11152..11185
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    11223..11271
                     /note="synthetic polyadenylation signal"
     polyA_signal    11280..11756
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11791..11810)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11835..11883
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    12057..12090
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      12107..12116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12246..12264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12514..12533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     12708..12731
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             13550..13606
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(13607..14224,14225..14230,14231..14266,14267..14269,
                     14270..14305,14306..14308,14309..14344,14345..14353)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    14396..14984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(14867..14878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15015..15222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    15236..15269
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        15296..15795
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(15331..15354)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     15728..15747
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(16443..16459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16443..16459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16456..16478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16467..16483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16491..16497,16498..16515,16516..16521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16536..16557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16674..16691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16845..17433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16925..16944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17604..18395,18396..18464))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18227..18246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18465..18569)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     18637..18655
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttaat
      481 tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg agagataatg
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     1681 ttgggggggg gctgtccctc tagcgagctc ggtacccaga gccccatcct cactgactcc
     1741 gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga
     1801 tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg
     1861 tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca
     1921 tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc
     1981 agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc
     2041 cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat
     2101 ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt
     2161 gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc
     2221 cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat
     2281 gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca
     2341 ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat
     2401 aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg
     2461 cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg
     2521 ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct
     2581 ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca
     2641 acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc
     2701 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     2761 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     2821 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     2881 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagg ggtacccggg
     2941 gatccaccgg tatcgattcg gccggccgac gtcggcagtg aaaaaaatgc tttatttgtg
     3001 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagtttcga
     3061 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3121 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     3241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     3301 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     3361 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     3421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     3481 gaccgatcca gcctccgcgg ccccgaattc gagctcggta cccggggatc aaattcaatt
     3541 aaatataact tcgtatagca tacattatac gaagttattc gcgatgaata aatgaaagct
     3601 tgcagatccc ctcagcacca tggctagcgg cagcctcgga gtttgaatag atagaataaa
     3661 atatctttat tttcattcca tctgtgtgtt ggttttttgt gtgagatcta cgggtggcat
     3721 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     3781 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     3841 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     3901 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     3961 atctccgcct cctgggttca agcgattctc ctgcctcagg ctcccgagtt gttgggattc
     4021 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     4081 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4141 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gcctcagcgc
     4201 tgtaagtctg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
     4261 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
     4321 gattggagct acgggggtgg gggtggggtg ggattagata aatgcctgct ctttactgaa
     4381 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
     4441 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
     4501 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
     4561 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
     4621 cattctcagt attgttttgc caagttctaa ttccatcaga aagcttttaa ttaactgaat
     4681 agctaacata acttcgtata gcatacatta tacgaagtta tcacgcgtgc cgccaccatg
     4741 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
     4801 cgggatctgt acgacgatga cgataaggat ctcgccacca tggtcgactc atcacgtcgt
     4861 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac
     4921 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcca catccgccac
     4981 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     5041 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa
     5101 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5161 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     5221 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5281 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     5341 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5401 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5461 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     5521 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5581 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5641 ctgccggacc aactgactga agagcagatc gcagaattta aagaggcttt ctccctattt
     5701 gacaaggacg gggatgggac aataacaacc aaggagctgg ggacggtgat gcggtctctg
     5761 gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt
     5821 gacggcacaa tcgacttccc tgagttcctg acaatgatgg caagaaaaat gaaatacagg
     5881 gacacggaag aagaaattag agaagcgttc ggtgtgtttg ataaggatgg caatggctac
     5941 atcagtgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt aacagatgaa
     6001 gaggttgatg aaatgatcag ggaagcagac atcgatgggg atggtcaggt aaactacgaa
     6061 gagtttgtac aaatgatgac agcgaagtaa acgcgttacg taaagcttgt cgataatcaa
     6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6481 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
     6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgaca ccgagcgctg
     6721 ctcgaggcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6901 aagggggagg attgggaaga caatagcagg catgctgggg acacgtggga tcctctagag
     6961 ccccatcctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc
     7021 aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg
     7081 gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg
     7141 gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg
     7201 gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca
     7261 attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt
     7321 ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag
     7381 cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct
     7441 tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca
     7501 gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga
     7561 tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca
     7621 cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt
     7681 tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg
     7741 cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct
     7801 ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg
     7861 agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt
     7921 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     7981 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     8041 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     8101 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     8161 ccctctagag ccccatcctc actgactccg tcctggagtt ggatgagaga taatggcctt
     8221 acgttgtgcc aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg
     8281 tgctgcagtg gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa
     8341 gaactgtggg gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt
     8401 tggtcttggg gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat
     8461 ctacatggca attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag
     8521 gcaagctctt ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca
     8581 cagggaccag cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc
     8641 tctttgtcct tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga
     8701 gagtgtccca gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt
     8761 atccccaaga tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc
     8821 aacaatgtca cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac
     8881 aggggaggtt tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact
     8941 gaacaggttg cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct
     9001 ggatgtggct ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg
     9061 gatctggatg agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta
     9121 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa
     9181 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     9241 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     9301 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     9361 ggggggctgt ccctctagag tcgacggcgc gccctgcagg aatctagtta ttaatagtaa
     9421 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9481 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9541 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9601 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9661 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9721 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     9781 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     9841 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgccr
     9901 ggsggggsgg ggsggggsgr ggggsggggc ggggcgaggc ggagaggtgc ggcggcagcc
     9961 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
    10021 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
    10081 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
    10141 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
    10201 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
    10261 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
    10321 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
    10381 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
    10441 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
    10501 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
    10561 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
    10621 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
    10681 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
    10741 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
    10801 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
    10861 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
    10921 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
    10981 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
    11041 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
    11101 cattttggca aagaattgat ttgataccgc gggccccgcg tggacggtac cataacttcg
    11161 tataaagtat cctatacgaa gttatccatg gctagcggca gcctcggagt ttgaatagat
    11221 agaataaaat atctttattt tcattccatc tgtgtgttgg ttttttgtgt gagatctacg
    11281 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
    11341 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
    11401 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
    11461 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
    11521 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcaggct cccgagttgt
    11581 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
    11641 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
    11701 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctgc
    11761 ctcagctaat tgagtagggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    11821 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
    11881 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
    11941 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
    12001 aaggcccagg gctcgcagcc aacgtcgggg cggcaggcct cgcgagggtt tctagaataa
    12061 cttcgtataa agtatcctat acgaagttat acgcgcgcgt actagcgcca ccatggtgag
    12121 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
    12181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
    12241 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
    12301 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
    12361 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
    12421 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
    12481 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
    12541 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
    12601 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
    12661 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
    12721 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
    12781 cctgttcctg gggcatggta ccggcagcac cggcagcggc agctccggca ccgcctcctc
    12841 cgaggacaac aacatggccg tgattaagga gtttatgagg tttaaagtca ggatggaggg
    12901 cagcatgaac gggcacgagt ttgagattga aggagagggg gaagggaggc cctatgaggg
    12961 cacccagaca gcaaagctga aagtgacaaa gggcggccct ctgccattcg catgggacat
    13021 cctgtctcct cagtttatgt atggaagtaa agcctacgtg aaacaccctg ctgacattcc
    13081 agattacaag aaactgagct tcccagaggg gtttaagtgg gaaagagtga tgaacttcga
    13141 agacgggggc ctggtgactg tcacccagga cagctctctg caggacggca ctctgatcta
    13201 taaggtgaaa atgcgcggca ccaattttcc ccctgatggc ccagtcatgc agaagaagac
    13261 aatgggctgg gaggcttcaa cagaaaggct gtaccccaga gacggcgtgc tgaagggaga
    13321 gatccaccag gcactgaagc tgaaagatgg agggcattat ctggtcgaat tcaaaaccat
    13381 ctacatggcc aagaaacccg tgcagctgcc tggctactat tacgtcgaca caaagctgga
    13441 tattactagc cataacgaag actacaccat tgtggagcag tatgagcgga gcgaggggag
    13501 gcaccatctg tttctgtacg gcatggacga gctgtacaag ggaagcggag ctactaactt
    13561 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgt ctagactgga
    13621 caagagcaaa gtcataaact ctgctctgga attactcaat gaagtcggta tcgaaggcct
    13681 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
    13741 gaagaacaag cgggccctgc tcgatgccct ggcaatcgag atgctggaca ggcatcatac
    13801 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
    13861 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
    13921 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
    13981 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
    14041 attggaggat caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
    14101 tatgccccca cttctgagac aagcaattga gctgttcgac catcagggag ccgaacctgc
    14161 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
    14221 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
    14281 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
    14341 gctccccggg taactaagta aggatcaaat ggcgcgttac gtaaagcttg tcgataatca
    14401 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    14461 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    14521 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    14581 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    14641 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    14701 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    14761 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
    14821 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    14881 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    14941 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgac accgagcgct
    15001 gctcgaggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    15061 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    15121 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    15181 caagggggag gattgggaag acaatagcag gcatgctggg gacacgtggg atccggtgcc
    15241 agggcgtgcc cttgggctcc ccgggcgcgt ttgggcggcc gcatcgaatt ctaccgggta
    15301 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
    15361 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
    15421 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
    15481 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
    15541 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
    15601 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
    15661 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
    15721 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
    15781 tctccgggcc tttcgacctg caatcgccgc tagccgccac catgaaaaag cctgaactca
    15841 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    15901 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    15961 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    16021 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    16081 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    16141 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    16201 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    16261 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    16321 acgacaccgt cagtgtaagt gctagcccct aggcctgaat cgaagttcct attcttcaaa
    16381 aggtatagga acttcgagct cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca
    16441 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    16501 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    16561 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    16621 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    16681 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    16741 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    16801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    16861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    16921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    16981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    17041 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    17101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    17161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    17221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    17281 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    17341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    17401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    17461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    17521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    17581 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    17641 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    17701 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    17761 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    17821 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    17881 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    17941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    18001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    18061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    18121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    18181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    18241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    18301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    18361 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    18421 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    18481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    18541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    18601 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    18661 cgtc
//
