LOCUS       sequence-22732-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS
  TITLE     Small ubiquitin-related modifier (SUMO)-specific proteases: 
            profiling the specificities and activities of human SENPs.
  JOURNAL   J Biol Chem. 2007 Sep 7. 282(36):26217-24.
  PUBMED    17591783
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Sep 7. 282(36):26217-24."
COMMENT     Sequence Label: pET28a-SENP6 (catalytic domain)
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttccctctag aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcaaacaa
      121 gaatttcagt tttttgatga agaagaagaa actggagaaa accacaccat cttcattggc
      181 ccagtagaaa agttgatagt atatccacca cctccagcta agggaggcat ctctgttacc
      241 aatgaggacc tgcactgtct aaatgaagga gaatttttaa atgatgttat tatagacttt
      301 tatttgaaat acttggtgct tgaaaaactg aagaaggaag acgctgaccg aattcatata
      361 ttcagttctt ttttctataa acgccttaat cagagagaga ggagaaatca tgaaacaact
      421 aatctgtcaa tacagcaaaa acggcatggg agagtaaaaa catggacccg gcacgtagat
      481 atttttgaga aggattttat ttttgtaccc cttaatgaag ctgcacactg gtttttggct
      541 gttgtttgtt tccccggttt ggaaaaacca aagtatgaac ctaatcctca ttaccatgaa
      601 aatgctgtca tacagaaatg ttcaactgta gaggacagtt gtatttcttc ttcagccagt
      661 gaaatggaga gttgttcaca aaactcttct gccaagccct gtaattaaga agatgctaaa
      721 ccaa
//
