LOCUS       sequence-227320-       17699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target dgCre into the endogenous mouse Calb1 locus at the stop 
            codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 17699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Calb1-T2A-dgCre targeting vector
FEATURES             Location/Qualifiers
     source          1..17699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(176..193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(347..935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(427..446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(1046..1501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1183..1204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1395..1414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(1543..2334,2335..2403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2166..2185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2404..2495)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2514..2541)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    7022..7055
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             7374..7427
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7926..7928,7929..8639)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7926..7928,7929..8639)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8229..8248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8576..8597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8661..9689
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(9696..9713)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9702..9909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9962..9995
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        10022..10521
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10057..10080)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10454..10473
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        10538..10585
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10604..11407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10667..10686)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11277..11296
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11417..11878
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    12672..12792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12709..12728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12762..12781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtttaaactt aattaatcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
       61 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccct atgcggtgtg
      121 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
      181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1021 aaaggatctt cacctagatc cttttaaatt gtaaacgtta atattttgtt aaaattcgcg
     1081 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1141 tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1201 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1261 ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca
     1321 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1381 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1441 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1681 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     2161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2461 atacatattt gaatgtattt agaaaaataa acaaaagagt ttgtagaaac gcaaaaaggc
     2521 catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg
     2581 cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc
     2641 tactcaggag agcgatcgcg atctagttgt cttggtagac agtataacaa tattagaatt
     2701 tatggtcaat gaatttgtaa aatgccgtgt cagtacagaa cactgaaaaa aaaatgtact
     2761 tccaaaaaag aattcttaga ccatctcaaa cttttctttt tgctttagaa aggactctct
     2821 aaacctctat gcactatctt taacatggcc tttttctcaa aaatatttta agatgctaca
     2881 tttggcacat ttagtattag cattaccttc attatggcaa tatttactgc tttctaaaaa
     2941 tgtgaatgtt tcccaaatat gtctgagtgc atctcagtat gtgatattta agtacaatag
     3001 tgagttagga aatattgtta agcttctagt acccagtgga agctaaataa tttgagattg
     3061 ttcaaaataa cttagtcttg ttatccactt actgcttttt tttttttaat tttgtgcttt
     3121 aaaataaaaa aataaaataa aaaaacaggc tggaaacgta gttcagtatt aaagagctta
     3181 cattgcaggc actcaagatg tttgattttt gaacgtttgt ctccttcatt caataattta
     3241 taactcagtg ttccaaccaa taacaatgcc tttcagctga aactatttga ttcaaataat
     3301 gacggaaagc tggaactgac agagatggcc aggtaagttt cagaattgca caaatgttat
     3361 tagaataaac agaatttcat ctaaaattcc aacctattaa catatttttt tctctctctc
     3421 tcaaaaggtt actaccagtg caggaaaatt tccttcttaa attccaggta gttggctttt
     3481 ctctacagta tataataata tctttcccta aaaactcaaa tggtaggata tttgtcaaga
     3541 gaaacaagct gaaatacatt atcactattt gactgaactt ctcttctgac tcctagggaa
     3601 tcaaaatgtg tgggaaagag ttcaataagg cttttgagtt atatgatcag gtaaaaatgt
     3661 tttcttccct tttttcttta aaaagaactt atgagaacaa tacttaacat gaaatctatc
     3721 ctcttgtgat tttaagtgtg acatgtatca tatatatata tatatatata tatatatata
     3781 tatatatata tatatatata tgcaaggctg tatggcagtt ctttagagct cactcatctt
     3841 acatatttga gactggacac tcattgctgg cttccccttt tttctgcttc cccttccctg
     3901 acatccacta ttctgctgct tccaagagtt tctttagttt agaaatattg tataaccacc
     3961 ctcctctgat aagcttattt caccttgtat gttctcaagg ttcacctatc ttataataca
     4021 ctacagagtg tcctttttaa acacaaaata atagagtgtg gtatatggat ttatgatctc
     4081 tttatatgta taatttcatt aggctgtttc cacatcttag ctattgtgaa aatgctgcat
     4141 gaaaatggaa tgttaatacc actaagagat cctgaccttt tggataaaac agcagaactg
     4201 tgatatttta taacatatgt tagttgtatt tttaatttgt tggtgtgtca acactgttgt
     4261 accattttga ttttctgtta acagtgtgca ggtgttccca gtttcttcac accctcacca
     4321 acactttcct cctttgcttg tatggtaatg gtcattcttg cagttgtgaa atgatacctc
     4381 gtttgttttc atttgcattt ctatgatgaa tactaaccct gatcaccttt tcataaactg
     4441 attagccatt tatatctttt cttggagaag tatctattca cattttagcc cacttttaaa
     4501 tcaaattagt tttatgctga tgaaagacag aagatatgac ttacatcttt cgatattaca
     4561 ttcctttagg taaggaatca gtgccttacc tggatggtct gccaatgcct tctcctgtct
     4621 gcaggctgcc tttaccctag atggttcttt gtaatatcta tgcttagctt cactgtcttc
     4681 ctgtagtttg tgctctgtgc tcataactca caagctcact gctaagacca atatcatgaa
     4741 ctttctacct attttatatg ggtatgttgt ctggtttggg ctttggtttt gacacaaggt
     4801 cccatgatgg ctggcctgac actcactata ttaaccaggc tggccttgaa ctcacagacc
     4861 catttcttat gcctcccaag agctaagatt aaagatgtgg atcaccacac ttggctctag
     4921 gagttttata tcttcataat ttaaatattt gcattatatt aattttgccc agataaaaaa
     4981 cagaggtcca ggttttttcc actttggcat gtggatacag ttttcctcat actttttgtt
     5041 aaagagaata tcttgtccct atactcagcc tcctagagaa agatcagttg attcgatgcc
     5101 tgtggacaaa ttccaaaaga aatattctat tctattctat tctattctat tctattctat
     5161 tctattcatc tctattcatc tctattgtct ctattcgtct ctattcaatc agtctctaag
     5221 taaataccat accattttag ctactgtaac cgagtacatt ttgaaatcag aaacttctgt
     5281 tgatttttca catttgtttg tttgtttgtt gcttttgcag ggaagcatgc cacaacacgt
     5341 cttcgttcca gagctatctt atcagtctga cacttccatt ttcatccttt attctttttc
     5401 tctaaactat ttcagttttg ggtctttgtg attatgtatg cttttagttg tatcttttat
     5461 atttcttctt aaaatactat tggagtttag aaacggatta tgctgatctt tagaattaaa
     5521 tggtatgaac actttaacaa gattaagtct tccagtccat aagcttaata atccgttggc
     5581 ttatgtccga tatctttcac cagagcttta caactgtcag tatacatgtg ttttactttc
     5641 atcgctaagt ttactcttaa gaagtttatt atatttttag tactctccca agaatgactt
     5701 taatatctgt cttgaaacag aactgatttt tagattttga tttttatatt ttatgacgtt
     5761 cctaaattta cttaatgcca acaggtttta tcttcagaat gttctatatt taaaaatcac
     5821 attattactt ccttctccat tttttaatct acaattttac cctctggaaa acatggaaac
     5881 cactcaccaa agaaaacaag agccacaaac taaatgttca caatttttaa aaagcatggg
     5941 tagctgaaat tacacaacca agtcattcgt tcaaccaaaa acttgtacag tatatctcac
     6001 atcacatgtt acactagcat gagccatgta cagtatatct cacatcacat gttacactag
     6061 catgagccat gtgcagtata tctcacatca catgttacac tagcatgagc catgtgcagt
     6121 atatctcaca tcacatgtta cactagcatg agccatgtac agtgtagctc acatcacatg
     6181 ttacactagc atgagccatg tcactgtgag gatctgaatg aggaacctaa accccctgtg
     6241 ccacaagatt agttgtaaat agaaaacaat aatgatattc cctgacaaag ttcaatgaaa
     6301 attaaggtag aggagacatt tcacagaagt gtctcacatt aaaaaaaaaa atagaacttt
     6361 atgtatgaga atagattggg tgcaagtttg ttagtccatg gttttggcct ctaatttctc
     6421 tattcctgga actagactct atataactgt tgcccactgg tgtgacatta agccaaccac
     6481 agcttcccca agctaaaatg ttctcctttg tcataggaat atatttagtc ttacttattt
     6541 acttattcca acggaagata cttcaaacat aaaatgaaat attaaatgtg agcatgcttt
     6601 gaaattttca atccatttca caaatgtaaa cttagctatt ttggaactgt taagggttct
     6661 accccaacac tgagttaggg tacccaccaa tggggatttt tttttctgag ggctctagtt
     6721 acttctaata tatactgttt tatttcttat accccaggat ggcaacggat acatagatga
     6781 aaatgagctg gatgctttgc tgaaagatct gtgtgagaag aacaaacagg taatttagta
     6841 gtcatgtcag ttacttccta agcagacttt agggaactgc ccagaaacaa ccacacaact
     6901 gttgtggaaa tatactgaaa gaatttgcag aagacaggag tggattttaa ttcttaattc
     6961 aaattgagtc tgtcgacctc tagaagtaag ctttgagctc aggaattctt ggatcctgtg
     7021 cgaagttcct attcttcaaa tagtatagga acttctttcg gccggccaac acaaatcagt
     7081 aaactgtgac aatcagattt gaattagaaa caacctggtg agtattcata tttataaaag
     7141 atctcttctt aatggactgg tgtagcaagc agtttctcaa gttttatcac tggccagtgg
     7201 ttaattaaca tccgtcttta attggattac aatgctatct atgtcctata caggaattgg
     7261 atattaacaa tattactaca tacaagaaga acataatggc cttgtcggat ggagggaagc
     7321 tgtaccgaac agaccttgct cttattcttt ctgctggaga caacggaagc ggcgagggca
     7381 gaggaagtct tctgacatgc ggagacgtgg aagagaatcc cggccctgcc ccaggctcaa
     7441 tgatctctct gattgccgct ctggccgtgg actacgtgat cgggatggaa aacgctatgc
     7501 catggaatct gcccgccgat ctggcttggt tcaagaggaa caccctgaac aagccagtga
     7561 tcatgggcag acacacttgg gagtccattg gacggcccct gcctggacgc aagaacatca
     7621 ttctgagctc ccagccctct accgacgaca gggtgacatg ggtgaaaagt gtggacgaag
     7681 ccattgccgc ttgcggagat gtgcccgaga tcatggtcat cggcggaggg agagtgatcg
     7741 agcagttcct gcctaaggcc cagaaactgt acctgactca cattgacgct gaggtggaag
     7801 gggacaccca ttttcctgat tatgagccag acgattggga aagcgtgttc tccgagtttc
     7861 acgacgccga tgctcagaat tctcatagtt attgctttga gatcctggaa aggagaggcg
     7921 cgccagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     7981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     8041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     8101 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     8161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     8221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     8281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     8341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     8401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     8461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     8521 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     8581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggatgag ctgtacaagg
     8641 gcaagaagaa gaggaaggtg tccaatttac tgaccgtaca ccaaaatttg cctgcattac
     8701 cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc
     8761 aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat
     8821 ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc
     8881 ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc
     8941 taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac
     9001 tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc
     9061 tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct
     9121 gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag
     9181 ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa
     9241 tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc
     9301 tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga
     9361 ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc
     9421 agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg
     9481 ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag
     9541 ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct
     9601 ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa
     9661 tggtgcgcct gctggaagat ggcgattagc tagcgcctcg actgtgcctt ctagttgcca
     9721 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     9781 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     9841 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     9901 tgctggggaa cgcgccgtaa aagcttggca tagatcttat tgcagaagcc atatgtttta
     9961 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtttgg atgcatttat cgaattctac
    10021 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
    10081 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
    10141 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
    10201 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
    10261 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
    10321 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
    10381 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
    10441 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
    10501 tcctcatctc cgggcctttc gacctgcagc agcacgtgtt gacaattaat catcggcata
    10561 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgggat cggccattga
    10621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    10681 ctgggcacaa cagacgatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    10741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
    10801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    10861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    10921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    10981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    11041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
    11101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
    11161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    11221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    11281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    11341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    11401 cttctgaggg gatccgctgt aagtctgcag aaattgatga tctattaaac aataaagatg
    11461 tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc
    11521 tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat
    11581 gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca tagttggata
    11641 tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca ctcacgatct
    11701 atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc
    11761 taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct
    11821 ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaaag
    11881 cttacctagg cctgaatcga agttcctatt cttcaaaagg tataggaact tcggttacaa
    11941 gacaggttta aggagaccaa tagaaactgg gcatgtggag acagagaaga ctcttgggtt
    12001 tctgataggc actgactctc tctgcctatt ggtctatttt cccaccctta ggcgtccgtc
    12061 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
    12121 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
    12181 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
    12241 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
    12301 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
    12361 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
    12421 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
    12481 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
    12541 actcgtccga gggcaaagga atagtgatca taatcagcca taccacattt gtagaggttt
    12601 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    12661 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    12721 caaatttcac aaataaagca ttttttcact gcattctagt cgtggtttgt ccaaactcat
    12781 caatgtatct taccccaact ggggtaacct ttgagttctc tcagttgggg gaaaggcgcg
    12841 gccgcacgcg tgcatgcagt tggtgaccac aaccacttgc tagtgataca ttgtatctaa
    12901 aaccataact gtgcgctata aagaagtagg ctgtattttc ttttatatct gtaaattcta
    12961 ctgcatatag agaactattc aggatgtgtg gcacattctt ttctgcttgt ttctacactg
    13021 tttgtaatgt acagtttttg taagcatata aatgaaaaga agaaagacca tgcttaggcc
    13081 agtcagtaca atcaattttg aaaaatgaat ctaccatggt tttgcgttta taaatacaga
    13141 tgaacacttg gatttccccg aaaatctacc atctcaaaac ttctagtgtc agattgtaaa
    13201 tgaatatctg tcattgagta aaagaataac tcatggcaag ccaactgttt ttattttagg
    13261 caaccatagg actgtcaccc aaagcacctc tgtgctgctt ctatctggcg gaagggatgg
    13321 gcttctgcct ctgaagcacc aaatatcaac agttaattat ggctttattc tgaaacgatc
    13381 tccctagaga tttagtgtac tgatcagtga catgtctact gcttaaatat aaaccacact
    13441 attgactcca gctggcttgg taagggaagg tagccagatg acacataact atgtctgatt
    13501 ctatgcctat atttccaaga agtctactgc cagagagtat gaccatagcc cattttctaa
    13561 attattttca tgtgttccag atgacaatta ttctagtaaa ctgctgtttt atgtcatatt
    13621 ctctgtgtgc tctctgatta aactcaatgt actgagtatt ttggtgtcta gtttgcctac
    13681 ttcctggatt ttctatgtag aactgttcat ttccaccaag ggtatctgct gcctctgaaa
    13741 atattttttt ctagctataa ctactctatt tttaatatgt aattgaattt taatgtaaga
    13801 ttcacagcaa cctgattatt gaatgttatt tcatcaaaac ttgtgtattc tgtggattct
    13861 atggttcata ttgagatcag cattcaaaat agctctattt ctatctggag aattccagat
    13921 gagttgaaga cagtaacaga agagaaatac tagtgtaatc atttatctta ttagcaaatg
    13981 atattctaaa accttcactg acccacatgt aactctgttt cgtgtatcct ttagctagtg
    14041 tgtcctctgc tggttattgg tgacaaaaat ctaattaatt gcctggattc tttattgtga
    14101 agcaagatac aatgacaaag tctatgaaat ttatagttat cacctaatgc aggtttgggt
    14161 caggttggat ttaagcactt tttttcaatt gttgttcata aaaataacaa caaaaccaag
    14221 tatggactct ttccctcaag aatgaatata aaaggaatct gtcacacaca aaaacaaaca
    14281 aataaataac aacagaaacc cactatatcc caggatgggg agaagtgata cactggcttt
    14341 acttcggcac gtatttggaa gcagtgactt tagactgaat ttaactacca agaataaagg
    14401 aaattacatt actctttcat atttgtttct accccaaagt gttaattgtc atgtaatctg
    14461 ttatcaatta gggtagaaaa gtagatatcg tgaatcatta tagccgaagg actctaataa
    14521 aaattttgct cagtgaatga cttagaggta tgagcagttt taaagattag ccaacaataa
    14581 atcctagaat atgcaaatat ttagagattt ccttttcctc aagtgatttg gcaacaattt
    14641 ttatgcaagg tctgaaaaga aataagacaa agatgtgtta aactttattt atagaaaaaa
    14701 tgtctattca acaaaataga cattaaaaat gtctattcag tcaaaatagt accaagtttg
    14761 gcattcttac taatagtagt agaacaaatc ctataatagt cacagttttt ctgatgttat
    14821 ttttatggat ttgggggata ttcatctatt catttttgaa acagggtcta accacatagc
    14881 atatactggc cttgaactct tgatgcttct gccttagcta ccttagtgac aggattacag
    14941 atgtgtgcca ccgtacactg gtggtagcat ttctcaaaag gcctagagta ctgacagcaa
    15001 cagcagtcat atttctagta tcagaattat catgatggtg tcaggttcct gaacactatc
    15061 aaaattctta tactgtagta aggcaacata tccttttctt gggaaaagaa cagaaaaaaa
    15121 aagaataaaa agaatataaa tctagaaatg tgaatgtaaa accactggta caatgtctgc
    15181 ctcaaaacct tgaacaaaat acattttaag tatagtttca atatgaatat aaattgtatg
    15241 ttgttaaaac taaaggtata tctattgctc tgggcatcaa aatagttttt agccatttag
    15301 aagaaacatt gaaaaaccat actttgtaaa acacacatac acacacacat cactaagagt
    15361 gaaaaaaaat tgtaatacaa gtaactgaca aagacagata gccaaaatac agattattcc
    15421 taaatatcaa ctttaaaata tagcacaatt aataaatttg tgagaaaatt gaaaacaatt
    15481 aaatgttaca ataacattcc cctatccaat aaacgtaaaa aataaccaat tttattatta
    15541 atcataagaa gcaaaaactt taaaaatggg atcctaccat ctgctcctaa gaatgttgat
    15601 gaggccgtgg tatgggtaga aacacctcta ctatgtaata acattttaag tataaacact
    15661 ttggcagacc atttggcaat atctactaga tttgaacata cctcactaaa tattcattcc
    15721 atacagctgg acagtttcac accaacatac tatcaaccta ctagagaacg tgtataattt
    15781 cacctgacaa acaagttcag aacaacattg tattgaggag ggactcaaac tagagctgcc
    15841 aaactggggt acatacacac tcaatacatt aaaatgaatg ctatagacca attcagatgc
    15901 agaaacagct tctacacagg tagaaacggc cagaaacaga atagcaccaa ctgagttaca
    15961 tgtttcaaaa acaggtataa tgactgcact aaatgtcgat cccaaagagg atagtcccaa
    16021 ggaggaagta gcagttcctg atgaggaata agggtggggt ttctggattc ttggcaacat
    16081 tctatttcaa aatattatgt gtgtacttat tttgtgatga ttcattgttt atattcttct
    16141 taaattaaaa ggtttattaa aatgggcttt ttccatttta agaattttct aagttagctt
    16201 agaccctgtc attatcttta agatatgaac tcacgagatc gctaactgca tcacagatgt
    16261 gagcttgact tcatgacctg gtacaagcca tataacctcc tggaccatta gctggaaaag
    16321 cagagagcct gagagtctcg tcaaagactt ctgctgctgc acatttctct tactcaaagt
    16381 caattgtgct cttactgcat caggttacac aagaaacact ggaaacaatg actgtcgctc
    16441 tatcttgaga cactaaaatt tttacatggc aattgtgagt gacacttacc aataaccctg
    16501 cttcagttga cagtgttaaa gctttcatca gtgaaatgaa aggatgtgga ccaaaagtac
    16561 caagagagtg agtggccatg atcattctta gagcaacacc ttgatgtagt gcacagccca
    16621 agggtggcca ctctgttgat tcatgaatag ttttctttca taaaaagctt agtaggggaa
    16681 attctcatga tctttctttg gatccccctc tgccatgctt gcaacctttg gccctttgtt
    16741 catctctcat aataacatgt aataattaga acattcagtg atttaaaaaa agtacacaag
    16801 ctcatacaag tcaaaaaatt taaatacctc actatcaaca gataaaacac aaaattctta
    16861 agtatagaaa tataatcata ctcatatgct agccaagcac tctaacataa agccaaaccg
    16921 ctcaatacat cttatgtgaa ctatgatatg tttcttatta agtaggtaaa tattttttgt
    16981 tgatagcaga catttcagta ctcaggggtt ataaatacat gtacatgttt ggaacaaatg
    17041 tgtgaaaatg gtttatcatg ctaaagctgc ctaacctttg atcaatatgg ggaaactggg
    17101 tagatggtac aaatggttaa aatgattgcc accgaatcat agggggcaaa gtaaggtgtt
    17161 ctgctttgta atccagtatg gaaaacagag gcaggcagat ccctgcactc cctggccaga
    17221 cagcctagat taaaagacaa acaccaggtc caatgcaggg accctacttc aaaaacaagg
    17281 tggatcattc ttgtggaata aaacttaaag ttgttctctg gcccccacaa acacatgtgc
    17341 tttcataagg tatacctata cctctgcata taatcacaca cactcacaca catgcattca
    17401 cacatgcaca cacacataca tatacacata atttctaata atctatcatg aagaacactt
    17461 caaattataa atataattta tatattaaaa aggatctgtt atatttgcaa cactgggaaa
    17521 atgttgtatg atcacagtta tatgtgcata tcacaaaggt gaacatttaa tgattttttt
    17581 ttaaaaaaag tactcaagct gatacaagtc aaaaaatgta aatacctcac tatcaacaga
    17641 tgaaacacaa aattcttgag tacagaaata taatcatact catatgctag ccaagtata
//
