LOCUS       sequence-227750-        6162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human cMYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     Sequence Label: pMXs-hc-MYC
FEATURES             Location/Qualifiers
     source          1..6162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..471
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     401..423
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     439..455
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    481..855
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     793..809
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    906..930
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             951..2270
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(2287..2311)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             2628..3097
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3227..3244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3398..3986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3478..3497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4150..4941,4942..5010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4773..4792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5011..5115)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5145..5738
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5801..6158
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag
       61 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
      121 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
      181 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
      241 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
      301 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
      361 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
      421 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg
      481 ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg
      541 acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag
      601 cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta
      661 cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta
      721 gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta
      781 gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga
      841 ccatcctcta gactgccgga tctagctagt taattaagga tcccagtgtg gtggtacggg
      901 aaatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgcccctca
      961 acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag ccgtatttct
     1021 actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg cagcccccgg
     1081 cgcccagcga ggatatctgg aagaaattcg agctgctgcc caccccgccc ctgtccccta
     1141 gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc tcccttcggg
     1201 gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag atggtgaccg
     1261 agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac gacgagacct
     1321 tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc gccgccaagc
     1381 tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc
     1441 ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat ctgagcgccg
     1501 ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac gacagcagct
     1561 cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg gattctctgc
     1621 tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc catgaggaga
     1681 caccgcccac caccagcagc gactctgagg aggaacaaga agatgaggaa gaaatcgatg
     1741 ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc agagtctgga tcaccttctg
     1801 ctggaggcca cagcaaacct cctcacagcc cactggtcct caagaggtgc cacgtctcca
     1861 cacatcagca caactacgca gcgcctccct ccactcggaa ggactatcct gctgccaaga
     1921 gggtcaagtt ggacagtgtc agagtcctga gacagatcag caacaaccga aaatgcacca
     1981 gccccaggtc ctcggacacc gaggagaatg tcaagaggcg aacacacaac gtcttggagc
     2041 gccagaggag gaacgagcta aaacggagct tttttgccct gcgtgaccag atcccggagt
     2101 tggaaaacaa tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca gcatacatcc
     2161 tgtccgtcca agcagaggag caaaagctca tttctgaaga ggacttgttg cggaaacgac
     2221 gagaacagtt gaaacacaaa cttgaacagc tacggaactc ttgtgcgtga aagggtgggc
     2281 gcgccgaccc agctttcttg tacaaagtgg tgatattcca gctgagcgcc ggtcgctacc
     2341 attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat
     2401 ttcgcgtaag gaaatccatt atgtactatt taaactcgag cggccgccag cacagtggtc
     2461 gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     2521 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     2581 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     2641 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     2701 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     2761 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     2821 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     2881 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     2941 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     3001 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     3061 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     3121 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     3181 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3241 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3301 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4081 tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg
     4141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4261 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcataatgaa agaccccacc
     5161 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     5221 ctgagaatag aaaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     5281 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     5341 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5401 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     5461 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     5521 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     5581 cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     5641 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     5701 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     5761 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc
     5821 tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt
     5881 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg
     5941 gccgcaaccc tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag
     6001 tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc cctaatagga gggatatgtg
     6061 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     6121 gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gc
//
