LOCUS       sequence-22776-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Hedgepeth CM, Deardorff MA, Rankin K, Klein PS
  TITLE     Regulation of glycogen synthase kinase 3beta and downstream Wnt 
            signaling by axin.
  JOURNAL   Mol Cell Biol. 1999 Oct . 19(10):7147-57.
  PUBMED    10490650
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Oct . 19(10):7147-57."
COMMENT     Sequence Label: Axin GID 1-2/pCS2MT
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(396..425)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(435..464)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(474..503)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(513..542)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(552..581)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(631..649)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(632..649)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acgactcact atagttctag aggctcgagt ccagaggaga catccccatc atccccttct
       61 tcttcagctc ggacgcgttt aagtcgctct tcaagcttct gttctgcttc acggtctctc
      121 aggaccccct ccaagcgact tataagctca gcagcaaatt tttcaggatc gacgtgaata
      181 tcctttggca tgtgatatgt acgaggaatg tgaggtagcg gtccacgacc atttgcgttg
      241 gcactctctt gcatttctct gcgatagtgt tttcggagtc tgtacggtgg gatcccatct
      301 acgctgctgt cagttaatga catggtatcg gcatcgctgg acatactctg ttgctcgctc
      361 tccatggtga ggtcgcccaa gctctccatt tcattcaagt cctcttcaga aatgagcttt
      421 tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatttc attcaagtcc
      481 tcttcagaaa tgagcttttg ctccatttca ttcaagtcct cttcagaaat gagcttttgc
      541 tccatttcat tcaagtcctc ttcagaaatg agcttttgct ccatagcttt aaatcgatgg
      601 gatcctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatc aagcttgctc
      661 cgacgtcccc aggcagaatg gcggttccct aaacgagcat tgcttatata gacctcccat
      721 tangcacgcc taccgcccat ttac
//
