LOCUS       sequence-22778-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Hedgepeth CM, Deardorff MA, Rankin K, Klein PS
  TITLE     Regulation of glycogen synthase kinase 3beta and downstream Wnt 
            signaling by axin.
  JOURNAL   Mol Cell Biol. 1999 Oct . 19(10):7147-57.
  PUBMED    10490650
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Oct . 19(10):7147-57."
COMMENT     Sequence Label: Axin GID 5-6/pCS2MT
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..64
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             74..103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             113..142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             152..181
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             191..220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    369..503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(393..412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     447..466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(616..636)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(616..634)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(668..690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    679..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(703..709,710..727,728..733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cctgtctttt gcggacccat cgatttaagc tatggagcaa aagctcattt ctgaagagga
       61 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
      121 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
      181 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg
      241 cgacctcacc atggagattc acgtcgatcc tgaaaaattt gctgctgagc ttataagtcg
      301 cttggagggg gtcctgagag accgtgaagc actcgagcct ctagaactat agtgagtcgt
      361 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      541 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
      601 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag
      721 cataaagtgt aaagcctggg gtgcctaatg agtgagctac ctcacattaa ttgcgttgcg
      781 ctcactgcc
//
