LOCUS       sequence-227789-        8720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human Braf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Li Y, Takahashi M, Stork PJ
  TITLE     Ras-mutant cancer cells display B-Raf binding to Ras that activates 
            extracellular signal-regulated kinase and is inhibited by protein 
            kinase A phosphorylation.
  JOURNAL   J Biol Chem. 2013 Sep 20;288(38):27646-57. doi: 
            10.1074/jbc.M113.463067. Epub 2013 Jul 26.
  PUBMED    23893412
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.463067"; journalName: "J Biol Chem"; date: 
            "2013-09-20- 20"; volume: "288"; issue: "38"; pages: "27646-57"
COMMENT     Sequence Label: GFP-Braf R188L
FEATURES             Location/Qualifiers
     source          1..8720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        168..547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        548..751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     702..722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     796..815
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        796..814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(839..841,842..844,845..1555)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(839..841,842..844,845..1555)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(884..905)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1145..1164)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1492..1513
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4206..4224)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4207..4224)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4244..4261)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4250..4474
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4520..4948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4607..4626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4817..4838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4957..4977)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4962..5291
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5142..5277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5204..5223
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5358..6152
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5412..5431)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6022..6041
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6326..6447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6363..6382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6417..6436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6496..6512)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6496..6512)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6509..6531)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6520..6536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6544..6550,6551..6568,6569..6574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6589..6610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6727..6744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6898..7486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6978..6997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7657..8448,8449..8517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8280..8299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8518..8622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8697..8716)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
       61 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      121 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      421 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      481 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      541 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      661 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      721 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      781 ctggcttatc gaaattaata cgactcacta tagggagacc caagctttct agaccaccat
      841 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      901 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      961 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1021 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1081 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1141 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1201 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1261 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1321 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1381 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1441 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1501 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggcggc
     1561 cgctcgggcc ccggctctcg gttataagat ggcggcgctg agcggtggcg gtggtggcgg
     1621 cgcggagccg ggccaggctc tgttcaacgg ggacatggag cccgaggccg gcgccggcgc
     1681 cggcgccgcg gcctcttcgg ctgcggaccc tgccattccg gaggaggtgt ggaatatcaa
     1741 acaaatgatt aagttgacac aggaacatat agaggcccta ttggacaaat ttggtgggga
     1801 gcataatcca ccatcaatat atctggaggc ctatgaagaa tacaccagca agctagatgc
     1861 actccaacaa agagaacaac agttattgga atctctgggg aacggaactg atttttctgt
     1921 ttctagctct gcatcaatgg ataccgttac atcttcttcc tcttctagcc tttcagtgct
     1981 accttcatct ctttcagttt ttcaaaatcc cacagatgtg gcacggagca accccaagtc
     2041 accacaaaaa cctatcgtta gagtcttcct gcccaacaaa cagaggacag tggtacctgc
     2101 aaggtgtgga gttacagtcc gagacagtct aaagaaagca ctgatgatgc tgggtctaat
     2161 cccagagtgc tgtgctgttt acagaattca ggatggagag aagaaaccaa ttggttggga
     2221 cactgatatt tcctggctta ctggagaaga attgcatgtg gaagtgttgg agaatgttcc
     2281 acttacaaca cacaactttg tacgaaaaac gtttttcacc ttagcatttt gtgacttttg
     2341 tcgaaagctg cttttccagg gtttccgctg tcaaacatgt ggttataaat ttcaccagcg
     2401 ttgtagtaca gaagttccac tgatgtgtgt taattatgac caacttgatt tgctgtttgt
     2461 ctccaagttc tttgaacacc acccaatacc acaggaagag gcgtccttag cagagactgc
     2521 cctaacatct ggatcatccc cttccgcacc cgcctcggac tctattgggc cccaaattct
     2581 caccagtccg tctccttcaa aatccattcc aattccacag cccttccgac cagcagatga
     2641 agatcatcga aatcaatttg ggcaacgaga ccgatcctca tcagctccca atgtgcatat
     2701 aaacacaata gaacctgtca atattgatga cttgattaga gaccaaggat ttcgtggtga
     2761 tggaggatca accacaggtt tgtctgctac cccccctgcc tcattacctg gctcactaac
     2821 taacgtgaaa gccttacaga aatctccagg acctcagcga gaaaggaagt catcttcatc
     2881 ctcagaagac aggaatcgaa tgaaaacact tggtagacgg gactcgagtg atgattggga
     2941 gattcctgat gggcagatta cagtgggaca aagaattgga tctggatcat ttggaacagt
     3001 ctacaaggga aagtggcatg gtgatgtggc agtgaaaatg ttgaatgtga cagcacctac
     3061 acctcagcag ttacaagcct tcaaaaatga agtaggagta ctcaggaaaa cacgacatgt
     3121 gaatatccta ctcttcatgg gctattccac aaagccacaa ctggctattg ttacccagtg
     3181 gtgtgagggc tccagcttgt atcaccatct ccatatcatt gagaccaaat ttgagatgat
     3241 caaacttata gatattgcac gacagactgc acagggcatg gattacttac acgccaagtc
     3301 aatcatccac agagacctca agagtaataa tatatttctt catgaagacc tcacagtaaa
     3361 aataggtgat tttggtctag ctacagtgaa atctcgatgg agtgggtccc atcagtttga
     3421 acagttgtct ggatccattt tgtggatggc accagaagtc atcagaatgc aagataaaaa
     3481 tccatacagc tttcagtcag atgtatatgc atttgggatt gttctgtatg aattgatgac
     3541 tggacagtta ccttattcaa acatcaacaa cagggaccag ataattttta tggtgggacg
     3601 aggatacctg tctccagatc tcagtaaggt acggagtaac tgtccaaaag ccatgaagag
     3661 attaatggca gagtgcctca aaaagaaaag agatgagaga ccactctttc cccaaattct
     3721 cgcctctatt gagctgctgg cccgctcatt gccaaaaatt caccgcagtg catcagaacc
     3781 ctccttgaat cgggctggtt tccaaacaga ggattttagt ctatatgctt gtgcttctcc
     3841 aaaaacaccc atccaggcag ggggatatgg tgcgtttcct gtccactgaa acaaatgagt
     3901 gagagagttc aggagagtag caacaaaagg aaaataaatg aacatatgtt tgcttatatg
     3961 ttaaattgaa taaaatactc tctttttttt taaggtgaaa aaaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4081 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4141 aaaaaaaaaa aaaaaaaccc gaattcgtcg acctcgaggg atccgggccc tctagagggc
     4201 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     4261 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4321 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4381 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4441 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4501 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4861 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4921 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4981 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5041 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5101 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5221 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5281 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     5341 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5401 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5461 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5521 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5581 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5641 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5701 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5761 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5821 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5881 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5941 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6001 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6061 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6121 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6181 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6241 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6301 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6361 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6421 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6481 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6541 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6601 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8641 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8701 atctgctctg atgccgcata
//
