LOCUS       sequence-227940-       11334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Smpd4 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Smpd4
FEATURES             Location/Qualifiers
     source          1..11334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6081..6630
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6225..6242)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6452..6471
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6633..7232
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6633..6652)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7129..7149
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7256..7844
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7309..7329)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7727..7738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8052..8685
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8814..8830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8814..8830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8827..8849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8838..8854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8862..8868,8869..8886,8887..8892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8907..8928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9045..9062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9216..9801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9296..9315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9972..10763,10764..10832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10595..10614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10833..10937)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10985..11119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11022..11041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11076..11095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggcgttccct cacctacaac
     3481 agcctagttt ccttctggct agcctgaaag ctgactctat aaataagccc tttgcacaac
     3541 gttgccaaga tttggtgaaa gtcattgagg attttccagc caaggaattg catgccgtct
     3601 tcccatggct ggtggaaagc atctttggca gcctggatgg tgtccttgtt ggttggaatc
     3661 tccgatgcct acagggacgt gtgaaccctg tggagtacag cacagcgatg gaatttctag
     3721 acccaagtgg tccaatgatg aagttggttt ataaacttca agctgaagac tataactttg
     3781 attttcctgt ctcctgtctt cctggccctg tgaaggcatc cattcaagag aatgtcctcc
     3841 ctgacagtcc tctgtaccac aacaaagtcc agtttccccc gaccggaggc cttggcctga
     3901 acctggccct caatccattt gaatactata tgttctactt tgctttgagc ctcatctctc
     3961 aaaagccaat gtctatgacc ctccatgtcc gtacttcgga ctgtgcctat ttcaccctgg
     4021 tggataggta cctatcatgg ttcctaccca ctgaaggcag cgtacctcca ccactctgct
     4081 ccagtccagg aggctccagc ccctcaccag ctcccagaac accagccatg ccctttgcct
     4141 cctatggcct ccacactagc ctcttgaagc gacacatctc tcatcagaca tctgtgaatg
     4201 cagaccctgc atcccatgag atctggcggt cagaaactct cctccaggtt tttgtggaaa
     4261 tgtggcttca tcattactcc ctggagatgt accaaaaaat gcagtcccct catgccaagt
     4321 tggaggttct acactaccga ctcactgtct ccagcgccct ccacagccct gcccaaccca
     4381 gcctccaggc cctccacgcc taccaagagt ccttcacgcc tactgaagag cacgtgctgg
     4441 tggtacgcct gctgctgaag cacctgcatg cctttgccaa cagcctgaag ccagatcagg
     4501 cttcgccatc tgcacactcc catgccacca gtccgctgga ggaattcaaa cgggccgcag
     4561 tgcctcgctt tgtccagcag aagctgtacg tcttcctgca gcactgtttt ggccactggc
     4621 ctctggatgc aaccttcaga gctgtcttgg agatgtggct gagctacctg cagccatgga
     4681 ggtatgcccc tgagaaacag gctcagggca gcgatcccca gccacggtgt gtgtcagaga
     4741 agtgggcacc cttcatccag gaaaacctgc tgatgtacac caagctgttt gtgagctttc
     4801 tgaaccgtgc cctacgcaca gaccttgtca gccccaagaa tgcactcatg gtcttccgag
     4861 tggctaaggt ctttgcccag cccaacctgg ctgagatgat ccagaaaggt gagcagctgt
     4921 tcctggagcc ggagctcatc atcccccacc gccaacaccg gctcttcaca gtcaccacca
     4981 gcttcctgtc accatggccc cctgttgtga cagatgcctc cttcaaggtg aagagccatg
     5041 tctatagcct agagggccaa gactgcaaat ataccccaat gtttggtcct gaaatccgaa
     5101 ccctggtctt gcgccttgct cagctcatca cacaggccaa gcagactgcc aagtccatct
     5161 ctgatcagta tgtggaaagc ccaactggcc gctccttcct gtcatggctg acctttggcc
     5221 tcacagacac aaatagctgc tacccagcca atgacctgga tgaaataggt caggacagca
     5281 tccgcaagac agacgagtac ttagaaaagg ccctggagta cctgcgccag atattccggc
     5341 tcagcgaagc ccagctggcc cagctcacgc ttgccctggg gagtgctcgg gacgagaatg
     5401 ggaagcagca gctcccagat tgcattgtgg gggaagaagg actcatcctt acacccttgg
     5461 gccgctacca gatcattaat ggtctgcgaa ggttcgagat tgagtaccag ggagacttag
     5521 agctgcagcc catccggagc tacgaaatca ccagtctggt ccgtgcactc ttccggctgt
     5581 cctctgccat caaccgtaga tttgcaggcc agatggcagc tctgtgctcc cggaatgact
     5641 tccttggcag cttctgtcgc taccacctca ctgagcctgc cttgagcaac agacatctgc
     5701 tgagccctgt agggcggagg caggtcacca atcctgcacg gggccccagg ctcagcctcc
     5761 gtttcctggg cagttaccgg acactgctcc tgctcctgat ggccttcttt gtggcttctc
     5821 tgttctgcat tgggcccctg tcctgctcct tgctcctggt cctaggatac gtcctctatg
     5881 ccatagctat gacactgctc actgaacggg ggaagctgca ccagctctga taatgggccg
     5941 cgacgatgtt taaacttcga aacgcgtgaa ttcctcgagg gcggccgctc tagagtcgac
     6001 gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc tagcgggatc
     6061 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6121 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6181 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6241 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6301 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     6361 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6421 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     6481 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6541 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6601 cgtggttttc ctttgaaaaa cacgataata ccatgaccga gtacaagccc acggtgcgcc
     6661 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6721 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6781 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6841 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6901 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6961 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7021 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     7081 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7141 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7201 cctggtgcat gacccgcaag cccggtgcct gacccgggcg gggcgcgtct ggaacaatca
     7261 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7321 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7381 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7441 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7501 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7561 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7621 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7681 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7741 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7801 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     7861 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     7921 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag tcacacctca
     7981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8041 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8101 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8161 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8221 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8281 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8341 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8401 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8461 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8521 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8581 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8641 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8701 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     8761 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8821 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9721 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     9781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10261 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10561 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10621 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10801 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10861 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10981 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11041 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11101 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    11161 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    11221 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    11281 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
