LOCUS       sequence-22800-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     Klein lab unpublished plasmids
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GSK-3beta-his (Xenopus)/pCS2+
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    99..233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(136..155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     190..209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(266..283)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggncgggcc natgcattgg cgccgcggcc gcgaattaaa aaacctccca cacctccccc
       61 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      121 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      181 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctacgtaa
      241 tacgactcac tatagttcta gatcagtggt ggtggtggtg gtgggaggag ttggaggcag
      301 atgcagaggc tgcgttgttg gtcgacccgc gctccccagt gtttgaatct gatgtcgatg
      361 ttgtatttga tgttgtggac acagcggcct ggttgcgagc gtgggccggg atgagaattg
      421 aggagagaga aggattgctt gacagctctt gagtggtgaa gttgaagaga gcggggaatt
      481 cgcgtccgtt gggaagtttc agattggggt cccgcagttc gtcgaagaag gagtgggcac
      541 aggcatccag gggggtgagg cgggaggtcg gggtgtattc cagcagtcgg ctgcacagag
      601 cgatgcgnan nntgnncnnn ncnnnnnnna acaccttann ccanggatgc gccttgatct
      661 gnggnaactt gacccgggat tgggtcattc cnncncnnnn ncnnnnccnn gngggncccc
      721 cnngactttt tntnccaccn ntnacccccc ccctcccccc cnnnaagagn gacnccaca
//
