LOCUS       sequence-228087-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: LbCas12a expression cassette for 
            dicotyledonous plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR1CB0013
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..783
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             874..4557
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4558..4605
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4612..4692
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      4708..4960
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5008..5032
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5188..6336)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6672..7260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6752..6771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7431..8222,8223..8291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8054..8073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8292..8396)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8483..9114
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9206..9230
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac
      841 aacattacaa ttacatttac aattatcgat acaatgagca agctggagaa gtttacaaac
      901 tgctactccc tgtctaagac cctgaggttc aaggccatcc ctgtgggcaa gacccaggag
      961 aacatcgaca ataagcggct gctggtggag gacgagaaga gagccgagga ttataagggc
     1021 gtgaagaagc tgctggatcg ctactatctg tcttttatca acgacgtgct gcacagcatc
     1081 aagctgaaga atctgaacaa ttacatcagc ctgttccgga agaaaaccag aaccgagaag
     1141 gagaataagg agctggagaa cctggagatc aatctgcgga aggagatcgc caaggccttc
     1201 aagggcaacg agggctacaa gtccctgttt aagaaggata tcatcgagac aatcctgcca
     1261 gagttcctgg acgataagga cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca
     1321 gccttcaccg gcttctttga taacagagag aatatgtttt ccgaggaggc caagagcaca
     1381 tccatcgcct tcaggtgtat caacgagaat ctgacccgct acatctctaa tatggacatc
     1441 ttcgagaagg tggacgccat ctttgataag cacgaggtgc aggagatcaa ggagaagatc
     1501 ctgaacagcg actatgatgt ggaggatttc tttgagggcg agttctttaa ctttgtgctg
     1561 acacaggagg gcatcgacgt gtataacgcc atcatcggcg gcttcgtgac cgagagcggc
     1621 gagaagatca agggcctgaa cgagtacatc aacctgtata atcagaaaac caagcagaag
     1681 ctgcctaagt ttaagccact gtataagcag gtgctgagcg atcgggagtc tctgagcttc
     1741 tacggcgagg gctatacatc cgatgaggag gtgctggagg tgtttagaaa caccctgaac
     1801 aagaacagcg agatcttcag ctccatcaag aagctggaga agctgttcaa gaattttgac
     1861 gagtactcta gcgccggcat ctttgtgaag aacggccccg ccatcagcac aatctccaag
     1921 gatatcttcg gcgagtggaa cgtgatccgg gacaagtgga atgccgagta tgacgatatc
     1981 cacctgaaga agaaggccgt ggtgaccgag aagtacgagg acgatcggag aaagtccttc
     2041 aagaagatcg gctccttttc tctggagcag ctgcaggagt acgccgacgc cgatctgtct
     2101 gtggtggaga agctgaagga gatcatcatc cagaaggtgg atgagatcta caaggtgtat
     2161 ggctcctctg agaagctgtt cgacgccgat tttgtgctgg agaagagcct gaagaagaac
     2221 gacgccgtgg tggccatcat gaaggacctg ctggattctg tgaagagctt cgagaattac
     2281 atcaaggcct tctttggcga gggcaaggag acaaacaggg acgagtcctt ctatggcgat
     2341 tttgtgctgg cctacgacat cctgctgaag gtggaccaca tctacgatgc catccgcaat
     2401 tatgtgaccc agaagcccta ctctaaggat aagttcaagc tgtattttca gaaccctcag
     2461 ttcatgggcg gctgggacaa ggataaggag acagactatc gggccaccat cctgagatac
     2521 ggctccaagt actatctggc catcatggat aagaagtacg ccaagtgcct gcagaagatc
     2581 gacaaggacg atgtgaacgg caattacgag aagatcaact ataagctgct gcccggccct
     2641 aataagatgc tgccaaaggt gttcttttct aagaagtgga tggcctacta taaccccagc
     2701 gaggacatcc agaagatcta caagaatggc acattcaaga agggcgatat gtttaacctg
     2761 aatgactgtc acaagctgat cgacttcttt aaggatagca tctcccggta tccaaagtgg
     2821 tccaatgcct acgatttcaa cttttctgag acagagaagt ataaggacat cgccggcttt
     2881 tacagagagg tggaggagca gggctataag gtgagcttcg agtctgccag caagaaggag
     2941 gtggataagc tggtggagga gggcaagctg tatatgttcc agatctataa caaggacttt
     3001 tccgataagt ctcacggcac acccaatctg cacaccatgt acttcaagct gctgtttgac
     3061 gagaacaatc acggacagat caggctgagc ggaggagcag agctgttcat gaggcgcgcc
     3121 tccctgaaga aggaggagct ggtggtgcac ccagccaact cccctatcgc caacaagaat
     3181 ccagataatc ccaagaaaac cacaaccctg tcctacgacg tgtataagga taagaggttt
     3241 tctgaggacc agtacgagct gcacatccca atcgccatca ataagtgccc caagaacatc
     3301 ttcaagatca atacagaggt gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc
     3361 ggcatcgata ggggcgagcg caatctgctg tatatcgtgg tggtggacgg caagggcaac
     3421 atcgtggagc agtattccct gaacgagatc atcaacaact tcaacggcat caggatcaag
     3481 acagattacc actctctgct ggacaagaag gagaaggaga ggttcgaggc ccgccagaac
     3541 tggacctcca tcgagaatat caaggagctg aaggccggct atatctctca ggtggtgcac
     3601 aagatctgcg agctggtgga gaagtacgat gccgtgatcg ccctggagga cctgaactct
     3661 ggctttaaga atagccgcgt gaaggtggag aagcaggtgt atcagaagtt cgagaagatg
     3721 ctgatcgata agctgaacta catggtggac aagaagtcta atccttgtgc aacaggcggc
     3781 gccctgaagg gctatcagat caccaataag ttcgagagct ttaagtccat gtctacccag
     3841 aacggcttca tcttttacat ccctgcctgg ctgacatcca agatcgatcc atctaccggc
     3901 tttgtgaacc tgctgaaaac caagtatacc agcatcgccg attccaagaa gttcatcagc
     3961 tcctttgaca ggatcatgta cgtgcccgag gaggatctgt tcgagtttgc cctggactat
     4021 aagaacttct ctcgcacaga cgccgattac atcaagaagt ggaagctgta ctcctacggc
     4081 aaccggatca gaatcttccg gaatcctaag aagaacaacg tgttcgactg ggaggaggtg
     4141 tgcctgacca gcgcctataa ggagctgttc aacaagtacg gcatcaatta tcagcagggc
     4201 gatatcagag ccctgctgtg cgagcagtcc gacaaggcct tctactctag ctttatggcc
     4261 ctgatgagcc tgatgctgca gatgcggaac agcatcacag gccgcaccga cgtggatttt
     4321 ctgatcagcc ctgtgaagaa ctccgacggc atcttctacg atagccggaa ctatgaggcc
     4381 caggagaatg ccatcctgcc aaagaacgcc gacgccaatg gcgcctataa catcgccaga
     4441 aaggtgctgt gggccatcgg ccagttcaag aaggccgagg acgagaagct ggataaggtg
     4501 aagatcgcca tctctaacaa ggagtggctg gagtacgccc agaccagcgt gaagcacaaa
     4561 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac
     4621 gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc
     4681 cccgactatg cctaagcttg tcaagcagat cgttcaaaca tttggcaata aagtttctta
     4741 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     4801 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     4861 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     4921 gataaattat cgcgcgcggt gtcatctatg ttactagatc gacgctacta ttgtcttctg
     4981 cacgaagtgg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     5041 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     5101 tccatttgta tgtgcatgcc aaccacaggg ttccccagat caggcgctgg ctgctgaacc
     5161 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
     5221 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
     5281 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
     5341 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
     5401 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
     5461 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
     5521 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
     5581 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
     5641 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
     5701 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
     5761 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
     5821 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
     5881 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
     5941 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
     6001 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
     6061 gatggtcatc gacttcgcca aacctgccgc ctcctgttca agacgacgcg aacgctccac
     6121 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
     6181 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
     6241 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
     6301 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
     6361 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
     6421 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
     6481 gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt
     6541 gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg
     6601 cttgtcgccg gcatcgttgc gccacatcta ggatctgcca ggaaccgtaa aaaggccgcg
     6661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7561 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaagg ttattgtctc
     8341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcacg
     8401 aattggccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
     8461 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
     8521 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     8581 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     8641 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     8701 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     8761 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     8821 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     8881 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     8941 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     9001 caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
     9061 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcgcaaag
     9121 gagatcctga tctgactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     9181 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     9241 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtgga tgcagtgggc
     9301 cccac
//
