LOCUS       sequence-228103-        5925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Zhao B, Ye X, Yu J, Li L, Li W, Li S, Yu J, Lin JD, Wang CY, 
            Chinnaiyan AM, Lai ZC, Guan KL
  TITLE     TEAD mediates YAP-dependent gene induction and growth control.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1962-71.
  PUBMED    18579750
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1962-71."
COMMENT     Sequence Label: pRK5-Myc-TEAD1
FEATURES             Location/Qualifiers
     source          1..5925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        258..637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        638..841
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     792..812
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     838..862
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1072..1090
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1072..1089
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1103..1122
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1168..1197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2469..2478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2482..2616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2519..2538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2573..2592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2685..3042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2685..2876
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2708..2728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2893..3028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3062..3079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3062..3078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3071..3093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3291..3746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3378..3397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3588..3609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3763..3782)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3882..3904
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3942..3960)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4028..4132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4133..4201,4202..4993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4351..4370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5164..5752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5653..5672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5906..5923
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      241 ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat caattacggg
      301 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      361 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      421 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      481 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      541 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      601 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      661 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      721 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      781 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      841 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      901 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
      961 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
     1021 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     1081 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     1141 ctgcacctcg gttctaagct gagaatggag caaaagctca tctcagagga agatctgaag
     1201 cttctgcagg tcgactctag agaaaggatg agtgactctg cagataagcc aattgacaat
     1261 gatgcagaag gggtctggag ccccgacatc gagcaaagct ttcaggaggc cctggctatc
     1321 tatccaccat gtgggaggag gaaaatcatc ttatcagacg aaggcaaaat gtatggtagg
     1381 aatgaattga tagccagata catcaaactc aggacaggca agacgaggac cagaaaacag
     1441 gtgtctagtc acattcaggt tcttgccaga aggaaatctc gtgattttca ttccaagcta
     1501 aaggatcaga ctgcaaagga taaggccctg cagcacatgg cggccatgtc ctcagcccag
     1561 atcgtctcgg ccactgccat tcataacaag ctggggctgc ctgggattcc acgcccgacc
     1621 ttcccagggg cgccggggtt ctggccggga atgattcaaa cagggcagcc aggatcctca
     1681 caagacgtca agccttttgt gcagcaggcc taccccatcc agccagcggt cacagccccc
     1741 attccagggt ttgagcctgc atcggcccca gctccctcag tccctgcctg gcaaggtcgc
     1801 tccattggca caaccaagct tcgcctggtg gaattttcag cttttctcga gcagcagcga
     1861 gacccagact cgtacaacaa acacctcttc gtgcacattg ggcatgccaa ccattcttac
     1921 agtgacccat tgcttgaatc agtggacatt cgtcagattt atgacaaatt tcctgaaaag
     1981 aaaggtggct taaaggaact gtttggaaag ggccctcaaa atgccttctt cctcgtaaaa
     2041 ttctgggctg atttaaactg caatattcaa gatgatgctg gggcttttta tggtgtaacc
     2101 agtcagtacg agagttctga aaatatgaca gtcacctgtt ccaccaaagt ttgctccttt
     2161 gggaagcaag tagtagaaaa agtagagacg gagtatgcaa ggtttgagaa tggccgattt
     2221 gtataccgaa taaaccgctc cccaatgtgt gaatatatga tcaacttcat ccacaagctc
     2281 aaacacttac cagagaaata tatgatgaac agtgttttgg aaaacttcac aattttattg
     2341 gtggtaacaa acagggatac acaagaaact ctactctgca tggcctgtgt gtttgaagtt
     2401 tcaaatagtg aacacggagc acaacatcat atttacaggc ttgtaaagga ctgagaattc
     2461 aatcgatggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
     2521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2581 tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg cgcagcacca
     2641 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     2701 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2761 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2821 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2881 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2941 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3001 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt aacagcttgg
     3061 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3121 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3181 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     3241 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3361 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3421 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3481 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3541 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3601 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3661 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3721 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3781 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3841 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3901 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3961 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4021 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4081 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4141 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4201 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4261 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4321 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4381 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4441 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4501 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4561 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4621 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4681 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4741 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4801 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4861 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4921 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4981 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5041 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5101 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5281 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5401 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5461 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5521 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5581 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5641 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5701 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5761 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5821 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5881 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gagga
//
