LOCUS       sequence-228104-        5943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a 
            c-fos minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5943)
  TITLE     Prywes Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3XMEF2-luc
FEATURES             Location/Qualifiers
     source          1..5943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    188..322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    346..480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(905..925)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1055..1120
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1371..3023)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     2939..2957
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3248..3382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3476..3610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3954..3971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4125..4713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4205..4224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4884..5675,5676..5744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5507..5526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5745..5849)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5917..5935
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      241 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      301 tataagctgc aataaacaag ttccgggtac cgggcccccc ctcgagatcc agacatgata
      361 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      421 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      481 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      541 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
      601 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
      661 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
      721 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
      781 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
      841 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
      901 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
      961 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
     1021 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
     1081 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
     1141 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcctct
     1201 agagcggccg ccaccgcggt ggagctcgaa ttccttttta gttacctata cctgatttta
     1261 ttattcgtta aaatagtaca gtgacccaca attacaaaat taccattcat caatttgcat
     1321 ataatattac aatagctaag aatttcgtca tcgctgaata cagttacatt ttacaatttg
     1381 gactttccgc ccttcttggc ctttatgagg atctctctga tttttcttgc gtcgagtttt
     1441 ccggtaagac ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg
     1501 gttgttactt gactggcgac gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc
     1561 tccaaaacaa caacggcggc gggaagttca ccggcgtcat cgtcgggaag acctgccacg
     1621 cccgcgtcga agatgttggg gtgttgtaac aatatcgatt ccaattcagc gggggccacc
     1681 tgatatcctt tgtatttaat taaagacttc aagcggtcaa ctatgaagaa gtgttcgtct
     1741 tcgtcccagt aagctatgtc tccagaatgt agccatccat ccttgtcaat caaggcgttg
     1801 gtcgcttccg gattgtttac ataaccggac ataatcatag gtcctctgac acataattcg
     1861 cctctctgat taacgcccag cgttttcccg gtatccagat ccacaacctt cgcttcaaaa
     1921 aatggaacaa ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt aatcagaata
     1981 gctgatgtag tctcagtgag cccatatcct tgtcgtatcc ctggaagatg gaagcgtttt
     2041 gcaaccgctt ccccgacttc tttcgaaaga ggtgcgcccc cagaagcaat ttcgtgtaaa
     2101 ttagataaat cgtatttgtc aatcagagtg cttttggcga agaatgaaaa tagggttggt
     2161 actagcaacg cactttgaat tttgtaatcc tgaagggatc gtaaaaacag ctcttcttca
     2221 aatctataca ttaagacgac tcgaaatcca catatcaaat atccgagtgt agtaaacatt
     2281 ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag tatccggaat gatttgattg
     2341 ccaaaaatag gatctctggc atgcgagaat ctgacgcagg cagttctatg cggaagggcc
     2401 acacccttag gtaacccagt agatccagag gaattcatta tcagtgcaat tgttttgtca
     2461 cgatcaaagg actctggtac aaaatcgtat tcattaaaac cgggaggtag atgagatgtg
     2521 acgaacgtgt acatcgactg aaatccctgg taatccgttt tagaatccat gataataatt
     2581 ttctggatta ttggtaattt tttttgcacg ttcaaaattt tttgcaaccc ctttttggaa
     2641 acaaacacta cggtaggctg cgaaatgttc atactgttga gcaattcacg ttcattataa
     2701 atgtcgttcg cgggcgcaac tgcaactccg ataaataacg cgcccaacac cggcataaag
     2761 aattgaagag agttttcact gcatacgacg attctgtgat ttgtattcag cccatatcgt
     2821 ttcatagctt ctgccaaccg aacggacatt tcgaagtatt ccgcgtacgt gatgttcacc
     2881 tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct cttcatagcc
     2941 ttatgcagtt gctctccagc ggttccatcc tctagaggat agaatggcgc cgggcctttc
     3001 tttatgtttt tggcgtcttc catggagatc ctctagagtc gactctagag gccgccggct
     3061 cagtcttggc ttctcagttg ctcgctgcag atgcggttgg agtacgaggc gccgcagcca
     3121 ctgcttttat aacaagcgtt ttatgaatga gtgcagatct cgagggctat ttttagggcc
     3181 tcgagggcta tttttagggc ctcgagggct atttttagca agcttgggct gcaggtcgac
     3241 tctagaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3301 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3361 tataagctgc aataaacaag ttaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtgata tggctgatcc
     3481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3601 taaacaagtt aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3661 ggttttttaa agcaagtaaa acctctacaa atgtgatatg gctgatcctg cctcgcgcgt
     3721 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3781 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3841 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatct gcattaatga
     3901 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3961 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5881 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5941 cgt
//
