LOCUS       sequence-228240-        4493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses the full length human SUMO1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Hietakangas V, Ahlskog JK, Jakobsson AM, Hellesuo M, Sahlberg NM, 
            Holmberg CI, Mikhailov A, Palvimo JJ, Pirkkala L, Sistonen L
  TITLE     Phosphorylation of serine 303 is a prerequisite for the 
            stress-inducible SUMO modification of heat shock factor 1.
  JOURNAL   Mol Cell Biol. 2003 Apr . 23(8):2953-68.
  PUBMED    12665592
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Apr . 23(8):2953-68."
COMMENT     Sequence Label: hSUMO1-HIS
FEATURES             Location/Qualifiers
     source          1..4493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    419..540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(456..475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     510..529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(574..591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(745..1333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(825..844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1504..2295,2296..2364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2127..2146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2365..2469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2537..2555
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2593..2615)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2715..2734
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2801..3256)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2938..2959)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3150..3169
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3382..3404
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3396..3413
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3397..3413
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3450..3470)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3558..3768
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3580..3776
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3627..3762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3807..4379
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4387..4406
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4434..4453
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4434..4452
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4464..4475
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 catcaccatc accatcacgg atccccggga atttccggtg gtggtggtgg aattctagac
       61 tccatggctg accaggaggc aaaaccttca actgaggact tgggggataa gaaggaaggt
      121 gaatatatta aactcaaagt cattggacag gatagcagtg agattcactt caaagtgaaa
      181 atgacaacac atctcaagaa actcaaagaa tcatactgtc aaagacaggg tgttccaatg
      241 aattcactca ggtttctctt tgagggtcag agaattgctg ataatcatac tccaaaagaa
      301 ctgggaatgg aggaagaaga tgtgattgaa gtttatcagg aacaaacggg gggtcattca
      361 acagtttaga tattgatcca tgcaatcacg aattcggatc cagatcttat taaagcagaa
      421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      541 tcatgtctgg tcgactctag actcttccgc ttcctcgctc actgactcgc tgcgctcggt
      601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1801 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2101 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2461 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2521 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     2581 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2641 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2701 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     2761 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3241 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3301 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     3361 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     3421 cgacttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaggaa
     3541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3721 ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt tgcaaaaagc
     3781 tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
     3841 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
     3901 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
     3961 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
     4021 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
     4081 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
     4141 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
     4201 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca
     4261 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
     4321 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4381 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg
     4441 actcactata gggcgaattc attaaagagg agaaattaac tatgagagga tcg
//
