LOCUS       sequence-228307-       14076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14076)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 14076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV/TO GFP-MDC1 (779-2)
FEATURES             Location/Qualifiers
     source          1..14076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..58
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    175..196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(211..216,217..234,235..241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     254..276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     273..289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     273..289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     308..328
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        310..328
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        356..582
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             583..763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    810..935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1428..1661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1846..1890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2157..2273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2296..2599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2600..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2754..2774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2805..2823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2826..2844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2885..2909
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2950..2959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2956..2958,2959..2961,2962..3672)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2956..2958,2959..2961,2962..3672)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3001..3022)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3262..3281)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3609..3630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10220..10244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10275..10863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10328..10348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10746..10757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10950..11183
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11255..11376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11292..11311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11346..11365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11416..11551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11571..11590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11572..11590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11600..11617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11600..11616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11609..11631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11758..12213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11845..11864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12055..12076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12239..12343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12344..12412,12413..13204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12562..12581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13375..13963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13864..13883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
       61 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      241 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      301 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      361 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      421 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      481 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      541 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      781 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      841 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      901 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      961 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1021 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1081 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1141 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1201 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1261 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1321 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1381 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1441 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1501 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1561 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1621 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1681 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1741 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1801 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1861 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1921 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1981 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2041 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2101 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2161 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2221 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     2281 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2341 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2401 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2461 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2521 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2581 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2641 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2701 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2761 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     2821 agatctccct atcagtgata gagatcgtcg actagtccag tgtggtggaa ttctgcagat
     2881 atcaacaagt ttgtacaaaa aagcaggctc caccatggga accaattcag tcgactagcg
     2941 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3001 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3061 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3121 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     3181 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3241 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3301 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3361 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3421 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3481 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3541 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3661 gagctgtaca agtccggccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     3721 cggtaccgcg ggcccatcat ggaggacacc caggctattg actgggatgt tgaagaagag
     3781 gaggagacag agcaatccag tgaatccttg aggtgtaacg tggagccagt agggcggcta
     3841 catatcttta gtggtgccca tggaccagaa aaagatttcc cactacacct cgggaagaat
     3901 gtggtaggcc gaatgcctga ctgctctgtg gccctgccct ttccatctat ctccaaacaa
     3961 catgcagaga ttgaaatctt agcctgggac aaggcaccta tcctccgaga ctgtgggagc
     4021 cttaatggta ctcaaatcct gagacctcct aaggttttga gccctggggt gagtcaccgt
     4081 ctgagggacc aggaattgat tctctttgct gacttgctct gccagtacca tcgcctggat
     4141 gtctctctgc cctttgtctc ccggggccct ctgacagtag aagagacacc cagagtacag
     4201 ggagaaactc aaccccagag gcttctgttg gctgaggact cggaggagga agtagatttt
     4261 ctttctgaaa ggcgtatggt aaaaaaatca aggaccacat cttcctctgt gatagttcca
     4321 gagagtgatg aagaggggca ttccccggtc ctgggcggcc ttgggccgcc ttttgccttc
     4381 aatttgaaca gtgacacaga tgtggaagaa ggtcagcaac cagccacaga ggaggcctcc
     4441 tcagctgcca gaagaggtgc cactgtagag gcaaagcagt ctgaagctga agttgtaact
     4501 gaaatccagc ttgaaaagga tcagccttta gtgaaggaga gggacaatga tacaaaagtc
     4561 aagaggggtg cagggaatgg ggtggttcca gctggggtga ttctggagag gagccaacct
     4621 cctggagagg acagtgacac agatgtggat gatgacagca ggcctcctgg aaggccagct
     4681 gaggtccatt tggaaagggc tcagcctttt ggcttcatcg acagcgacac tgatgcggaa
     4741 gaagagagga tcccagcaac cccagttgtc attcctatga agaagaggaa gatcttccat
     4801 ggagtaggta caaggggtcc tggagcacca ggcctggccc atctgcagga gagccaggct
     4861 ggtagtgata cagatgtgga agaaggcaag gccccacagg ctgtccctct ggagaaaagc
     4921 caagcttcca tggttatcaa cagcgataca gatgacgagg aagaagtctc agcagcgctg
     4981 actttggcac atctgaaaga gagccagcct gctatatgga acagagatgc agaagaggac
     5041 atgccccaac gtgtggtcct tctgcagcga agccaaacca ccactgagag agacagtgac
     5101 acagacgtgg aggaggaaga gctcccagtg gaaaatagag aagctgtcct caaggatcac
     5161 acaaagatta gagcccttgt tagagcacat tcagaaaagg accaacctcc ttttggggac
     5221 agtgatgaca gtgtggaagc agataagagc tcacctggga tccacctgga gagaagccaa
     5281 gcctccacca cagtggacat caacacacaa gtggagaagg aagtcccgcc agggtcagcc
     5341 attatgcata taaagaagca tcaggtgtct gtggagggga caaatcaaac agatgtgaaa
     5401 gcagttgggg gaccagcaaa gctgcttgtg gtatctctag aggaagcctg gcctctgcat
     5461 ggggactgtg aaacagatgc agaggagggc acctccctaa cagcctcagt agttgcagat
     5521 gtaagaaaga gccagcttcc agcagaaggg gatgctgggg cagagtgggc tgcagctgtt
     5581 cttaagcagg agagagctca tgaggtgggg gcccagggtg ggccacctgt ggcacaagtg
     5641 gagcaggacc tccctatctc aagagagaac ctcacagatc tggtggtgga cacagacact
     5701 ctaggggaat ccacccagcc acagagagag ggagcccagg tccccacagg aagggagaga
     5761 gaacaacatg tgggtgggac caaggactct gaagacaact atggtgattc tgaagatctg
     5821 gacctacaag ctacccagtg ctttctggag aatcagggcc tggaagcagt ccagagcatg
     5881 gaggatgaac ctacccaggc cttcatgttg actccacccc aagagcttgg cccttcccat
     5941 tgcagcttcc agacaacagg taccctagat gaaccatggg aggtcctggc tacacagcca
     6001 ttctgtctga gagagtctga ggactctgag acccagcctt ttgacacgca ccttgaggcc
     6061 tatggacctt gcctgtctcc acctagggca ataccaggag accaacatcc agagagccca
     6121 gttcacacag agccaatggg gattcaaggc agagggaggc agactgtgga taaagtcatg
     6181 ggtataccaa aagaaacagc agagagggtg ggccctgaga gagggccatt ggagagagaa
     6241 actgagaaac tgctaccaga aagacagaca gatgtgacag gagaggaaga attaaccaag
     6301 gggaaacagg acagagaaca aaaacagttg ttagctagag acacccagag acaagaatct
     6361 gacaaaaatg gggaaagtgc aagtcctgaa agagataggg agagtttgaa ggtagaaatt
     6421 gagacatctg aggaaataca agagaaacaa gtacagaagc agacccttcc aagcaaagca
     6481 tttgagagag aagtagagag accagtagca aacagagagt gcgatccagc cgagttagaa
     6541 gagaaggtgc ccaaagtgat cctggagaga gatacacaga gaggggagcc agagggaggg
     6601 agccaggacc agaaagggca ggcctccagc ccaacaccag agcctggggt gggggcgggg
     6661 gaccttccgg gacctacctc agcccccgta ccttctggga gccagtcagg tggaagggga
     6721 tccccagtga gccccaggag gcatcagaaa ggcctcctga attgcaagat gccacctgct
     6781 gagaaggctt ccaggatcag agctgctgag aaggtttcca ggggcgatca ggaatctcca
     6841 gatgcttgtc tgcctcctgc agtacctgaa gccccagccc caccccaaaa gccccttaac
     6901 tctcagagcc agaaacatct tgcacctccg ccccttcttt ctcccctttt accttctatc
     6961 aagccaaccg ttcgtaagac caggcaagat gggagtcagg aagctccaga ggctcccttg
     7021 tcctcagagc tggagccttt ccacccaaag cctaaaatta gaactcggaa gtcctccaga
     7081 atgacaccct ttccagctac ctctgctgcc cctgagcccc acccttccac ctccacagcc
     7141 cagccagtca ctcccaagcc cacatctcag gccactagga gcaggacaaa taggtcctct
     7201 gtcaagaccc ctgaaccagt tgtccccaca gcccctgagc tccagccttc cacctccaca
     7261 gaccagcctg tcacctctga gcccacatct caggttacta ggggaagaaa aagtagatcc
     7321 tctgtcaaga cccctgaaac agttgtgccc acagcccttg agctccagcc ttccacctcc
     7381 accgaccgac ctgtcacctc tgaacccacc tctcaggcta ctaggggaag aaaaaataga
     7441 tcctctgtca agacccctga accagttgtc cccacagccc ctgagctcca gccttccacc
     7501 tccacagacc agcctgtcac ttctgagccc acatatcagg ctactagggg aagaaaaaat
     7561 agatcctctg tcaagacccc tgaaccagtt gtgcccacag cccctgagct ccggccttcc
     7621 acctccacag accgacctgt cacccccaag cccacatctc ggaccactag gagcaggaca
     7681 aatatgtcct ctgtcaagac ccctgaaaca gttgtcccca cagcccctga gctccagatt
     7741 tccacctcca cagaccaacc tgtcacccct aagcccacat ctcggaccac taggagcagg
     7801 acaaatatgt cctctgtgaa gaaccctgaa tcaactgtcc ctatagcccc tgagctccca
     7861 ccttccacct ccacagagca gcctgtcacc cctgagccca catctcgggc tactagggga
     7921 agaaaaaata gatcctctgg caagacccct gaaacacttg tccccacagc ccctaagctc
     7981 gagccttcca cttccacaga ccaacctgtc actcctgagc ccacatctca ggccaccagg
     8041 ggcaggacaa ataggtcctc tgtgaagacc cctgaaacag ttgtccccac agcccctgag
     8101 ctccagcctt ccacctccac agaccagcct gttacccctg agcctacgtc tcaggctact
     8161 aggggaagaa cagatagatc ctctgtcaag actcctgaaa cagttgtccc cacagcccct
     8221 gagctacagg cttccgcctc cacagaccag cctgtcacct ctgagcccac atctcggacc
     8281 actaggggaa gaaaaaatcg gtcctctgtc aagacccctg aaacagttgt gcccgcagcc
     8341 cctgagctcc agcctcccac ctccacagac cgacctgtca cccctgagcc cacatctcgg
     8401 gccactaggg gcaggacaaa taggtcctct gtcaagaccc ctgaatcaat tgtccctata
     8461 gcccctgagc ttcagccttc cacctccaga aaccagcttg tcacccctga gcccacatct
     8521 cgggccacta ggtgcaggac aaataggtcc tctgtcaaga cccctgagcc agttgtcccc
     8581 acagcccctg agccccatcc taccacctcc acagaccagc ctgtcacccc caagctcaca
     8641 tctagggcca ctaggagaaa gacaaatagg tcctctgtca agactcccaa accagttgaa
     8701 ccagcagcct ctgatcttga gccttttacc cccacagacc agtccgtcac ccctgaggcc
     8761 atagctcagg gtggtcagag caaaacactg aggtcttcca cagtaagagc tatgccggtt
     8821 cctaccaccc ctgaattcca atctcctgtc accacagacc agcctatttc ccctgagcct
     8881 attactcaac ccagttgcat caagaggcag agagccgctg ggaaccctgg ctccctcgca
     8941 gctcccattg accataagcc ttgctctgca cccttggaac ctaaatccca ggcctcaagg
     9001 aaccaaagat ggggagcagt gagagcagct gaatccctta cagccattcc tgagcctgcc
     9061 tctccccagc ttcttgagac accaattcat gcctcccaga tccaaaaggt ggaaccagca
     9121 ggtagatcta ggttcacccc ggagctccag cctaaggcct ctcaaagccg caagaggtct
     9181 ttagctacca tggattcacc accacatcaa aaacagcccc aaagagggga agtctcccag
     9241 aagacagtga ttatcaagga agaggaagaa gatactgcag agaagccagg gaaggaagag
     9301 gatgtcgtga ctccaaaacc aggcaagaga aagagagacc aggcagagga ggagcccaac
     9361 agaataccaa gccgcagcct ccgacggacc aaacttaacc aagaatcaac agcccccaaa
     9421 gtgctcttca caggagtggt ggatgctcgg ggagagcggg ctgtgctggc actgggggga
     9481 agtctggctg gttcagcggc agaggcttcc cacctggtca ctgatcgcat ccgccggaca
     9541 gtcaagttcc tgtgtgccct ggggcgggga atccccattc tgtccctgga ctggctgcat
     9601 cagtcccgca aggctggttt cttcttaccc ccggatgaat atgtggtgac cgaccctgag
     9661 caagagaaga actttggctt tagccttcaa gacgcactga gcagggctcg ggagcgaagg
     9721 ctgctagagg gctatgagat ctatgtgacc cctggagtcc agccaccacc acctcagatg
     9781 ggagagatta ttagctgctg tggaggcaca tacctaccca gcatgcctcg gtcctataag
     9841 cctcagagag ttgtgatcac atgccctcag gacttccctc attgctccat tccactacgg
     9901 gttgggctgc ccctcctctc gcctgagttc ctgctgactg gagtgctgaa gcaggaagcc
     9961 aagccagagg cctttgtcct ctcccctttg gagatgtcat ccacctgaga actccactac
    10021 ccttttccct cccagaccac gaattagaag atatgtggaa gaaagaactc agggcgttag
    10081 aaaggattgg ggtatattga tacaacttgt cctggaacat gggtgggacc agaaatcttt
    10141 atgaataaat gaaaagataa gggatttgga agccacaggt tgttttttgt ttgtttgttt
    10201 gtttttttaa tggatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt
    10261 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
    10321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
    10381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    10441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    10501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    10561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    10621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
    10681 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    10741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    10801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    10861 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
    10921 tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca
    10981 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    11041 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    11101 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    11161 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
    11221 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
    11281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
    11401 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    11461 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    11521 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
    11581 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    11641 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    11701 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
    11761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    11821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    11881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    11941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    12001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    12061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    12121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    12181 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
    12241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    12301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    12361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    12421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    12481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    12541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    12601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    12661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    12721 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    12781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    12841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    12901 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    12961 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    13021 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    13081 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    13141 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    13201 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    13261 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    13321 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    13381 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    13441 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    13501 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    13561 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    13621 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    13681 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    13741 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    13801 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    13861 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    13921 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    13981 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    14041 ccctgattct gtggataacc gtattaccgc ctttga
//
