LOCUS       sequence-228315-       11111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 
            mismatch(pEPOR1CB0018).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 11111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0028
FEATURES             Location/Qualifiers
     source          1..11111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5033..5035,5036..5038,5039..5752)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5765..6017
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        6269..6344
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6356..6380)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6449..6473
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6782..7411
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7840..8913
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8979..9173
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9235..9339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9340..10134
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10305..10893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10794..10813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11029..11053
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa
      901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
      961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa
     2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat
     3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     3421 gtcttttctc gcagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc
     3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccgcgct
     3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaaggcac cacttatcga
     4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca
     5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcatcttc attcttaaga
     6061 tatgaagata atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta
     6121 tatgggaaag aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa
     6181 aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag
     6241 tagtgattgc tgttaatttg agagtccagt tttagagcta gaaatagcaa gttaaaataa
     6301 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctagaccca
     6361 gctttcttgt acaaagttgg cattacgctt tacgaggatg cacatgtgac cgagggacac
     6421 gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6481 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6541 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtcagcc gtgcggctgc
     6601 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     6661 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     6721 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     6781 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     6841 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     6901 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     6961 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     7021 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     7081 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     7141 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     7201 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     7261 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     7321 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     7381 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     7441 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     7501 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     7561 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     7621 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     7681 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     7741 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     7801 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     7861 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     7921 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     7981 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gtcgtcgatt
     8041 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     8101 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     8161 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     8221 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     8281 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     8341 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     8401 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     8461 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     8521 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     8581 tggatgtacc gcgagattac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     8641 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     8701 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     8761 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     8821 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     8881 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     8941 gccctagcag gggaaaaagg tcgaaaaagc ttctttcctg tggatagcac gtacattggg
     9001 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     9061 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     9121 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     9181 ggccagcgca cagccgaaca gctgcaaaaa gcgcccacat caaggctccg agtgcgcgga
     9241 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9301 ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca
     9361 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     9421 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     9481 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     9541 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     9601 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     9661 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     9721 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
     9781 aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat
     9841 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     9901 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     9961 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
    10021 ctcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
    10081 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca
    10141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10441 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10921 ttcctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc ctgtatcgag
    10981 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    11041 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    11101 gtactggggt t
//
