LOCUS       sequence-228373-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0062
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             896..916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4097..4144
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4151..4231
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             join(4238..4240,4241..4243,4244..4957)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4283..4304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4544..4563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4970..5222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        5476..5551
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_feature    5630..5654
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5963..6592
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7021..8094
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8160..8354
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8416..8520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8521..9315
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9486..10074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9975..9994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10210..10234
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggccccaaa
      901 gaagaagcgg aaggtcggta tccacggagt cccagcagcc aagcggaact acatcctggg
      961 cctggacatc ggcatcacca gcgtgggcta cggcatcatc gactacgaga cacgggacgt
     1021 gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg
     1081 gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg catagaatcc agagagtgaa
     1141 gaagctgctg ttcgactaca acctgctgac cgaccacagc gagctgagcg gcatcaaccc
     1201 ctacgaggcc agagtgaagg gcctgagcca gaagctgagc gaggaagagt tctctgccgc
     1261 cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac
     1321 cggcaacgag ctgtccacca aagagcagat cagccggaac agcaaggccc tggaagagaa
     1381 atacgtggcc gaactgcagc tggaacggct gaagaaagac ggcgaagtgc ggggcagcat
     1441 caacagattc aagaccagcg actacgtgaa agaagccaaa cagctgctga aggtgcagaa
     1501 ggcctaccac cagctggacc agagcttcat cgacacctac atcgacctgc tggaaacccg
     1561 gcggacctac tatgagggac ctggcgaggg cagccccttc ggctggaagg acatcaaaga
     1621 atggtacgag atgctgatgg gccactgcac ctacttcccc gaggaactgc ggagcgtgaa
     1681 gtacgcctac aacgccgacc tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac
     1741 cagggacgag aacgagaagc tggaatatta cgagaagttc cagatcatcg agaacgtgtt
     1801 caagcagaag aagaagccca ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga
     1861 ggatattaag ggctacagag tgaccagcac cggcaagccc gagttcacca acctgaaggt
     1921 gtaccacgac atcaaggaca ttaccgcccg gaaagagatt attgagaacg ccgagctgct
     1981 ggatcagatt gccaagatcc tgaccatcta ccagagcagc gaggacatcc aggaagaact
     2041 gaccaatctg aactccgagc tgacccagga agagatcgag cagatctcta atctgaaggg
     2101 ctataccggc acccacaacc tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg
     2161 gcacaccaac gacaaccaga tcgctatctt caaccggctg aagctggtgc ccaagaaggt
     2221 ggacctgtcc cagcagaaag agatccccac caccctggtg gacgacttca tcctgagccc
     2281 cgtcgtgaag agaagcttca tccagagcat caaagtgatc aacgccatca tcaagaagta
     2341 cggcctgccc aacgacatca ttatcgagct ggcccgcgag aagaactcca aggacgccca
     2401 gaaaatgatc aacgagatgc agaagcggaa cgcgaaaacc aacgagcgga tcgaggaaat
     2461 catccggacc accggcaaag agaacgccaa gtacctgatc gagaagatca agctgcacga
     2521 catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc cctctggaag atctgctgaa
     2581 caaccccttc aactatgagg tggaccacat catccccaga agcgtgtcct tcgacaacag
     2641 cttcaacaac aaggtgctcg tgaagcagga agaaaacagc aagaagggca accggacccc
     2701 attccagtac ctgagcagca gcgacagcaa gatcagctac gaaaccttca agaagcacat
     2761 cctgaatctg gccaagggca agggcagaat cagcaagacc aagaaagagt atctgctgga
     2821 agaacgggac atcaacaggt tctccgtgca gaaagacttc atcaaccgga acctggtgga
     2881 taccagatac gccaccgcac gtctgatgaa cctgctgcgg agctacttca gagtgaacaa
     2941 cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc agctttctgc ggcggaagtg
     3001 gaagtttaag aaagagcgga acaaggggta caagcaccac gccgaggacg ccctgatcat
     3061 tgccaacgcc gatttcatct tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat
     3121 ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg cccgagatcg aaaccgagca
     3181 ggagtacaaa gagatcttca tcacccccca ccagatcaag cacattaagg acttcaagga
     3241 ctacaagtac agccaccggg tggacaagaa gcctaataga gagctgatta acgacaccct
     3301 gtactccacc cggaaggacg acaagggcaa caccctgatc gtgaacaatc tgaacggcct
     3361 gtacgacaag gacaatgaca agctgaaaaa gctgatcaac aagagccccg aaaagctgct
     3421 gatgtaccac cacgaccccc agacctacca gaaactgaag ctgattatgg aacagtacgg
     3481 cgacgagaag aatcccctgt acaagtacta cgaggaaacc gggaactacc tgaccaagta
     3541 ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag tattacggca acaaactgaa
     3601 cgcccatctg gacatcaccg acgactaccc caacagcaga aacaaggtcg tgaagctgtc
     3661 cctgaagccc tacagattcg acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt
     3721 gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa gtgaatagca agtgctatga
     3781 ggaagctaag aagctgaaga agatcagcaa ccaggccgag tttatcgcct ccttctacaa
     3841 caacgatctg atcaagatca acggcgagct gtatagagtg atcggcgtga acaacgacct
     3901 gctgaaccgg atcgaagtga acatgatcga catcacctac cgcgagtacc tggaaaacat
     3961 gaacgacaag aggcccccca ggatcattaa gacaatcgcc tccaagaccc agagcattaa
     4021 gaagtacagc acagacattc tgggcaacct gtatgaagtg aaatctaaga agcaccctca
     4081 gatcatcaaa aagggcaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     4141 aaagggatcc tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta
     4201 cgcttaccca tacgatgttc cagattacgc tggttcgatg gtgagcaagg gcgaggagct
     4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     4441 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta caagtgagct tgtcaagcag atcgttcaaa
     4981 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5041 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5101 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5161 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5221 tcgacgctac tagaattcga gctcggagca tcttcattct taagatatga agataatctt
     5281 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa
     5341 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct
     5401 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attgtatgac
     5461 ctttaagaga gaagcgtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg
     5521 ccgtgtttat ctcgtcaact tgttggcgag atttttttac ttgtctcgct ttacgaggat
     5581 gcacatgtga ccgagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat
     5641 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
     5701 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     5761 gggagtcagc cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     5821 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     5881 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     5941 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     6001 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     6061 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     6121 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     6181 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     6241 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     6301 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     6361 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     6421 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     6481 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     6541 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     6601 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     6661 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     6721 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     6781 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     6841 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     6901 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     6961 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     7021 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     7081 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     7141 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     7201 gcagccggtg cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     7261 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     7321 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     7381 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     7441 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     7501 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     7561 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     7621 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     7681 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     7741 atcgagatcg agctagctga ttggatgtac cgcgagatta cagaaggcaa gaacccggac
     7801 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     7861 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     7921 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     7981 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     8041 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     8101 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct
     8161 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     8221 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     8281 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     8341 ctggcctgtg cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca
     8401 tcaaggctcc gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8521 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     8581 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     8641 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     8701 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     8761 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     8821 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     8881 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     8941 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     9001 gaggacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     9061 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     9121 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     9181 attgccttct gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta
     9241 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     9301 gatgaattgt tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10081 agcaacgcgg cctttttacg gttcctgctc ggatctgttg gaccggacag tagtcatggt
    10141 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    10201 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
    10261 cacattgcgg acgtttttaa tgtactgggg tt
//
