LOCUS       sequence-228412-        5060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Int5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 546 to 580)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-3
FEATURES             Location/Qualifiers
     source          1..5060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        546..580
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             2303..2314
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2756..2773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3063..3082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3678..3772)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(3870..3938,3939..4730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4734..4836)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(5013..5056)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtacctata atgtgtggat cacacaggaa agtactagat tgaacctcta
      421 ctacgagtga gttgagatta cagccttact cggtaccaaa ttccagaaaa gagacgcttt
      481 cgagcgtctt ttttcgtttt ggtcccttac gctattattg aagccagtct gttaccgaag
      541 tgaaattgac ggctagctca gtcctaggta cagtgctagc cagaggaagg aggctcgatg
      601 cctggtatga ccaccgaaac cggtccggat ccggcaggtc tgattgacct gttttgtcgt
      661 aaaagcaaag cagttaaaag ccgtgcaaat ggtgcaggtc agcgtcgtaa acaagaaatt
      721 agcattgcag cacaagaaac cctgggtcgt aaagttgcag cactgctggg tatgcaggtt
      781 cgtcatgttt ggaaagaagt tggtagcgca agccgttttc gtaaaggtaa agcacgtgat
      841 gatcagagca aagcactgaa agccctggaa agcggtgaag ttggtgcact gtggtgttat
      901 cgtctggatc gttgggatcg tggtggtgcc ggtgcaattc tgaaaattat cgaaccggaa
      961 gatggtatgc ctcgtcgtct gctgtttggt tgggatgaag ataccggtag tccggttctg
     1021 gatagcacca ataaacgtga tcgcggtgaa ctgattcgtc gtgcagaaga agcacgcgaa
     1081 gaagcagaaa aactgagcga acgtgttcgt gataccaaag cacatcagcg tgaaaatggt
     1141 gaatgggtta atgcccgtgc accgtatggt ctgcgtgttg ttctggttac cgttagtgat
     1201 gaagagggtg atgaatatga tgaacgtaaa ctggcagcag atgatgaaga tgcgggtggt
     1261 cctgatggtc tgaccaaagc agaagcagcc cgtctggttt ttaccctgcc ggttaccgat
     1321 cgtctgagct atgcaggcac cgcacatgca atgaataccc gtgaaattcc gagcccgacc
     1381 ggtggtccgt ggattgcagt taccgtgcgt gatatgattc agaatccggc atatgcgggt
     1441 tggcagacca caggtcgtca ggatggtaaa cagcgtcgtc tgacctttta taacggtgaa
     1501 ggtaaacgtg ttagcgttat gcatggtcct ccgctggtga ccgatgaaga acaagaagcc
     1561 gcaaaagcag ccgttaaagg tgaagatggt gttggtgttc cgctggatgg tagcgatcat
     1621 gatacccgtc gcaaacatct gctgagcggt cgtatgcgtt gtccgggttg tggtggtagc
     1681 tgtagctata gcggtaatgg ttatcgttgt tggcgtagca gtgtgaaagg tggttgtccg
     1741 gcaccgacct atgttgcacg taaaagcgtt gaagaatatg ttgcatttcg ttgggcagca
     1801 aaattagcag caagcgaacc ggatgatccg tttgttattg cagttgcaga tcgctgggca
     1861 gcactgaccc atccgcaggc aagcgaagat gaaaagtatg caaaagccgc agttcgtgaa
     1921 gccgaaaaaa atctgggtcg cctgctgcgt gatcgtcaga atggtgttta tgatggtccg
     1981 gcagaacagt tttttgcccc tgcatatcaa gaagcactga gcaccttgca ggcagccaaa
     2041 gatgcagtta gcgaaagcag cgcaagcgca gcagttgatg ttagctggat tgttgatagc
     2101 agcgattatg aagaactgtg gctgcgtgca accccgacca tgcgtaatgc aattattgat
     2161 acctgcatcg atgaaatttg ggttgcaaaa ggccagcgtg gtcgtccgtt tgatggtgat
     2221 gaacgcgtta aaatcaaatg ggcagcccgt acctaaaacg attacggatt gctctactgt
     2281 gactgaagtt tacaacccaa ttattgaagg ccgctaacgc ggcctttttt tgtttctggt
     2341 ctccctgagg cacagagatt tactttattc acgacttcag atacgcacag ctaacaccac
     2401 gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc
     2461 ataaggctcg tatgatatat tcagggagac cacaacggtt tccctctaca aataattttg
     2521 tttaacttta gtaatttcaa caaaataact aggattcgaa atacggtcta ctacagaagg
     2581 gtggtttatc ttactcaacg acgaacaata aggcctccct aacggggggc cttttttatt
     2641 gataacaaaa agatttccct cgtcacgcag taagtattta tcgtagaaga tcacttttat
     2701 cggtttccag aacaggtaag agccaatagt gattacttcg cgttatgcag gcttcctcgc
     2761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2821 cggtaatcct agctagggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3301 cccggtaaga cacgacttat cgccactggc agcagcttat cgccactggc agcagccact
     3361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3421 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     3481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3541 ggttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3661 tcatgacttg tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     3721 aacaaatcca gatggagttc tgaggtcatt actggatcta tcagcaggag tccaagcgag
     3781 ctcgatatca ctaaatacat tcaaatatct atccgctcat gagacaataa ccctgataaa
     3841 tgcttcaata atattgaaaa aggaagaata tgagtattca acatttccgt gtcgccctta
     3901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3961 taaaagatgc cgaagatcag ttgggtgcac gtgtgggtta catcgaactg gacctcaaca
     4021 gcggtaagat tcttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta
     4081 aagttctgct ctgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4201 ttacggacgg catgacagta cgcgaattat gcagcgctgc cataaccatg agtgataaca
     4261 cggcggccaa cttacttctg acaacgatcg gaggaccgaa ggagcttacc gcttttttgc
     4321 acaacatggg tgatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4381 taccaaacga cgagcgtgac accacgatgc ctgtagctat ggcaacaacg ttgcgcaaac
     4441 tcttaactgg cgaacttctt actctcgctt cccggcaaca attaatagac tggatggagg
     4501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4681 gaaatagaca gatcgctgag ataggtgcct ccctgattaa gcattggtaa taatttagct
     4741 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     4801 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg
     4861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4921 cctttcgtct tcacctcgag tgccacctga cgtctaagaa accattatta tcatgacatt
     4981 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     5041 cgctaaggat gatttctgga
//
