LOCUS       sequence-228485-        5126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ARF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Kahn, Richard
  TITLE     Kahn expression plasmids
  JOURNAL   unpublished
  PUBMED    2474826
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pJL16
FEATURES             Location/Qualifiers
     source          1..5126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(85..90,91..107,108..114)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    118..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(743..760)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      913..999
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1091..1118
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1137..1228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1229..1297,1298..2089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1447..1466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2260..2848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2749..2768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3002..3019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3034..3174
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3100..3119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3260..3282
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3276..3467)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4925..4944
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
       61 tggcaaatat tctgaaatga gctgttgaca attaatcatc cggctcgtat aatgtgtgga
      121 attgtgagcg gataacaatt ccacacagga aacagaccat gggcaatatc tttggaaacc
      181 ttctcaagag cctgattggg aagaaggaga tgcgcatcct gatggtgggc ctggatgccg
      241 caggaaagac caccatccta tacaagctga aactggggga gatcgtcacc accatcccta
      301 ccattgggtt caatgtggag acagtggagt ataagaacat cagctttaca gtgtgggatg
      361 tgggtggcca ggacaagatt cgacccctct ggagacacta cttccagaac acccaagggt
      421 tgatatttgt ggtcgacagc aatgatcggg agcgagtaaa tgaggcccgg gaagagctga
      481 tgagaatgct ggcggaggac gagctccggg atgctgtact ccttgtcttt gcaaacaaac
      541 aggatctgcc taatgctatg aacgctgctg agatcacaga caagctgggc ctgcattccc
      601 ttcgtcaccg taactggtac attcaggcca cctgtgccac cagcggggac gggctgtacg
      661 aaggcctgga ctggctggcc aatcagctca aaaacaagaa gtgaaagctt ggctgttttg
      721 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
      781 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      841 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      901 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      961 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     1021 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     1081 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     1141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1441 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     1501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1741 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2941 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3001 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3061 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     3121 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     3181 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3241 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3301 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     3361 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     3421 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     3481 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     3541 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     3601 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     3661 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     3721 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     3781 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     3841 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     3901 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
     3961 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     4021 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     4081 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     4141 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     4201 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta
     4261 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     4321 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     4381 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     4441 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     4501 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     4561 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     4621 ctacgagttg catgataaag aagacagtca taagtgcggt gacgatagtc atgccccgcg
     4681 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
     4741 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     4801 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     4861 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     4921 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     4981 ccacgatgcg tccggcgtag aggatccgga attctcatgt ttgacagctt atcatcgact
     5041 gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc
     5101 aggtcgtaaa tcactgcata attcgt
//
