LOCUS       sequence-228564-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast-targeted expression of CURT_fluoB controlled by the CaMV
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CTP-fluoB
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(931..933,934..1647)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(973..994)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1234..1253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1581..1602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1650..1674)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1994..2018
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3349..3978
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4407..5480
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5546..5741
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5977..5999)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6085..6225
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6140..6159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6240..6257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6411..6999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6491..6510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7245..8036)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8557..8581
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8674..8926)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8949..9521)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9563..9742)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggct tcctctatgc tctcttccgc tactatggtt
      481 gcctctccgg ctcaggccac tatggtcgct cctttcaacg gacttaagtc ctccgctgcc
      541 ttcccagcca cccgcaaggc taacaacgac attacttcca tcacaagcaa cggcggaaga
      601 gttaactgcg cttcttcaga agagacctcg tccattgata ccaacgaact catcacagat
      661 ttgaaggaaa agtgggatgg tcttgagaac aaatctactg tacttatata tggaggagga
      721 gccattgttg ctgtttggtt atcttccatt gttgttggtg ccatcaactc tgttcctctg
      781 cttccgaaag ttatggaact tgtcggacta gggtacactg gatggtttgt ctacagatac
      841 cttctcttca agtcaagcag aaaggaattg gctgaggata ttgaatcctt gaagaagaag
      901 atcgcaggaa gcgaaggagg tggtggatct gtgagcaagg gcgaggagct gttcaccggg
      961 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1021 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1081 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc
     1141 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1201 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1261 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1321 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1381 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1441 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1501 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
     1561 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1621 ctcggcatgg acgagctgta caagtaatcg ttcagctttc ttgtacaaag tggtgatatc
     1681 ccgcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     1741 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     1801 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     1861 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg
     1921 acgtcgggcc caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     1981 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2041 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     2101 catgccaacc acagggttcc cctcgggatc aaagtacttt aaagtacttt aaagtacttt
     2161 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     2221 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     2281 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     2341 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     2401 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     2461 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     2521 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     2581 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     2641 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     2701 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     2761 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     2821 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     2881 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     2941 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     3001 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     3061 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     3121 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     3181 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     3241 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     3301 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     3361 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     3421 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     3481 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     3541 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     3601 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     3661 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     3721 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     3781 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     3841 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     3901 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     3961 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     4021 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     4081 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     4141 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     4201 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     4261 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     4321 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     4381 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     4441 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     4501 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     4561 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     4621 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     4681 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     4741 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     4801 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     4861 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     4921 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     4981 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     5041 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     5101 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     5161 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     5221 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     5281 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     5341 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     5401 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     5461 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     5521 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     5581 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     5641 acatgtaagt gactgatata aaagagaaaa aaaggcgatt tttccgccta aaactcttta
     5701 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     5761 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     5821 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     5881 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     5941 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6001 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     6061 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     6121 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     6181 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     6241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6841 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatgat
     7081 atatctccca atttgtgtag ggcttattat gcacgcttaa aaataataaa agcagacttg
     7141 acctgatagt ttggctgtga gcaattatgt gcttagtgca tctaacgctt gagttaagcc
     7201 gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt gccgactacc
     7261 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     7321 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     7381 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
     7441 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     7501 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     7561 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     7621 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     7681 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     7741 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     7801 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     7861 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     7921 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     7981 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc ccccatgatg
     8041 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     8101 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
     8161 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     8221 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
     8281 accgaacagg cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct
     8341 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
     8401 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
     8461 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
     8521 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
     8581 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
     8641 cgccgaattg aattatcagc ttgcatgccg gtcgatctag taacatagat gacaccgcgc
     8701 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     8761 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     8821 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     8881 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct tgactctagg
     8941 ggtcatcatt aggggcaggg catgctcatg tagagcgcct gctcgccgtc cgaggcggtg
     9001 ccgtcgtaca gggcggtgtc caggccgcag agggtgaacc ccatccgccg gtacgcgtgg
     9061 atcgccggtg cgttgacgtt ggtgacctcc agccagaggt gcccggcacc ccgctcgcgg
     9121 gcgaactccg tcgcgagccc catcaacgcg cgcccgaccc cgtgcccccg gtgctccggg
     9181 gcgacctcga tgtcctcgac ggtcagccgg cggttccagc cggagtacga gacgaccacg
     9241 aagcccgcca ggtcgccgtc gtccccgtac gcgacgaacg tccgagagtc cgggtcgccg
     9301 tcctccccgt cgtccgattc gtcgtccgat tcgtcgtcgg ggaacacctt ggtcaggggc
     9361 gggtccaccg gcacctcccg cagggtgaag ccgtccccgg tggcggtgac gcggaagacg
     9421 gtgtcggtgg tgaaggaccc atccagtgcc tcgatggcct cggcgtcccc cgggacactg
     9481 gtgcggtacc ggtaagccgt gtcgtcaaga gtggtaccca tggtagatcc cctcgatcga
     9541 gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     9601 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     9661 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt
     9721 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     9781 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa ttcgagctct
     9841 cccatatggt cgactagagc caagctgatc tcctttgccc cggagatcac catggacgac
     9901 tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga taccatgttc
     9961 accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga cccacagatg
    10021 gttagagagg cctacgcggc aggtctcatc aagacgatct acccgagtaa taatctccag
    10081 gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc
    10141 atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg
    10201 attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc taagaaagta
    10261 gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct aacagaactc
    10321 gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga caagaagaaa
    10381 atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
    10441 gtctcagaag accaaagggc tat
//
