LOCUS       sequence-228667-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express CRX in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_CRX_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatggcgt atatgaaccc ggggccccac tattctgtca acgccttggc cctaagtggc
       61 cccagtgtgg atctgatgca ccaggctgtg ccctacccaa gcgcccccag gaagcagcgg
      121 cgggagcgca ccaccttcac ccggagccaa ctggaggagc tggaggcact gtttgccaag
      181 acccagtacc cagacgtcta tgcccgtgag gaggtggctc tgaagatcaa tctgcctgag
      241 tccagggttc aggtttggtt caagaaccgg agggctaaat gcaggcagca gcgacagcag
      301 cagaaacagc agcagcagcc cccagggggc caggccaagg cccggcctgc caagaggaag
      361 gcgggcacgt ccccaagacc ctccacagat gtgtgtccag accctctggg catctcagat
      421 tcctacagtc cccctctgcc cggcccctca ggctccccaa ccacggcagt ggccactgtg
      481 tccatctgga gcccagcctc agagtcccct ttgcctgagg cgcagcgggc tgggctggtg
      541 gcctcagggc cgtctctgac ctccgccccc tatgccatga cctacgcccc ggcctccgct
      601 ttctgctctt ccccctccgc ctatgggtct ccgagctcct atttcagcgg cctagacccc
      661 tacctttctc ccatggtgcc ccagctaggg ggcccggctc ttagccccct ctctggcccc
      721 tccgtgggac cttccctggc ccagtccccc acctccctat caggccagag ctatggcgcc
      781 tacagccccg tggatagctt ggaattcaag gaccccacgg gcacctggaa attcacctac
      841 aatcccatgg accctctgga ctacaaggat cagagtgcct ggaagtttca gatcttg
//
