LOCUS       sequence-228688-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express FOXA3 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_FOXA3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(856..867)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgctgggct cagtgaagat ggaggcccat gacctggccg agtggagcta ctacccggag
       61 gcgggcgagg tctactcgcc ggtgacccca gtgcccacca tggcccccct caactcctac
      121 atgaccctga atcctctaag ctctccctat ccccctgggg ggctccctgc ctccccactg
      181 ccctcaggac ccctggcacc cccagcacct gcagcccccc tggggcccac tttcccaggc
      241 ctgggtgtca gcggtggcag cagcagctcc gggtacgggg ccccgggtcc tgggctggtg
      301 cacgggaagg agatgccgaa ggggtatcgg cggcccctgg cacacgccaa gccaccgtat
      361 tcctatatct cactcatcac catggccatc cagcaggcgc cgggcaagat gctgaccttg
      421 agtgaaatct accagtggat catggacctc ttcccttact accgggagaa tcagcagcgc
      481 tggcagaact ccattcgcca ctcgctgtct ttcaacgact gcttcgtcaa ggtggcgcgt
      541 tccccagaca agcctggcaa gggctcctac tgggccctac accccagctc agggaacatg
      601 tttgagaatg gctgctacct gcgccgccag aaacgcttca agctggagga gaaggtgaaa
      661 aaagggggca gcggggctgc caccaccacc aggaacggga cagggtctgc tgcctcgacc
      721 accacccccg cggccacagt cacctccccg ccccagcccc cgcctccagc ccctgagcct
      781 gaggcccagg gcggggaaga tgtgggggct ctggactgtg gctcacccgc ttcctccaca
      841 ccctatttca ctggcctgga gctcccaggg gagctgaagc tggacgcgcc ctacaacttc
      901 aaccaccctt tctccatcaa caacctaatg tcagaacaga caccagcacc tcccaaactg
      961 gacgtggggt ttgggggcta cggggctgaa ggtggggagc ctggagtcta ctaccagggc
     1021 ctctattccc gctctttgct taatgcatcc
//
