LOCUS       sequence-22869-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     Zebrafish Expression Plasmids
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZE11 tiggywinkle hedgehog CMV
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        210..228
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     210..227
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     241..260
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
       61 ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt
      121 accgcctata gagtctatag gcccaccccc ttggcttcgt tagaacgcgg ctacaattaa
      181 tacataacct tatgtatcat acacatacga tttaggtgac actatagaat aacatccact
      241 ttgcctttct ctccacaggt gtccactccc aggtccaact gcacctcggt tctatcgatt
      301 gaattccccg gggatctgat gatcccccgg gctgcaggaa ttcggcacga ggcggtcgtt
      361 attgcacctg cttcggtagt ggtcgttggg cagcatccat ccggttcgcg cgagaagacc
      421 ctgagagtga ctaaacttta aagaccagaa gagataattc aaacgtcatg gacgtaaggc
      481 tgcatctgaa gcaatttgct ttactgtgtt ttatcagctt gcttctgacg ccttgtggat
      541 tagcctgtgg tcctggtaga ggttatggaa aacgaagaca cccaaagaaa ttaaccccgt
      601 tggcttacaa gcaattcatc cccaacgttg ctgagaaaac gcttggagcc agcggcaaat
      661 acgaaggcaa aatcacaagg aattcagaga gatttaaaga gctgattccg aattataatc
      721 ccgatatcat ctt
//
